IUPAC/Chemical Name
poly(ethyleneamine) with C15 lipid.
SMILES Code
NCCN(CCNCC(O)CCCCCCCCCCCCC)CCNCCN(CCNCCN(CCN(CCNCCNCC(O)CCCCCCCCCCCCC)CCNCC(O)CCCCCCCCCCCCC)CCNCC(O)CCCCCCCCCCCCC)CCNCCNCC(O)CCCCCCCCCCCCC
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
To be determined
Shelf Life
>2 years if stored properly
Drug Formulation
To be determined
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Biological target:
7C1 is a low-molecular weight, ionizable polymer that forms nanoparticles.
In vivo activity:
To test the ability of 7C1 nanoparticles to achieve effective gene silencing in the endothelium, Tie2 was selected as the target gene because of its putative endothelial cell specificity. After confirming sequence homology of the Tie2 siRNA target site in mouse and nonhuman primate, the efficacy of the Tie2 siRNA formulated in 7C1 nanoparticles was first analyzed in a dose-response study in mice. he particle sizes measured by dynamic light scattering of the Tie2 and Luc control 7C1 siRNA nanoparticles were 86.8 ± 2.6 nm and 109.6 ± 2.6 nm, respectively.
Reference: Sci Adv. 2018 Jun 27;4(6):eaar8409. https://pubmed.ncbi.nlm.nih.gov/29963629/
|
Solvent |
mg/mL |
mM |
| Solubility |
| To be determined |
0.0 |
0.00 |
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.
Preparing Stock Solutions
The following data is based on the
product
molecular weight
0.00
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
Formulation protocol:
1. Khan OF, Kowalski PS, Doloff JC, Tsosie JK, Bakthavatchalu V, Winn CB, Haupt J, Jamiel M, Langer R, Anderson DG. Endothelial siRNA delivery in nonhuman primates using ionizable low-molecular weight polymeric nanoparticles. Sci Adv. 2018 Jun 27;4(6):eaar8409. doi: 10.1126/sciadv.aar8409. PMID: 29963629; PMCID: PMC6021147.
In vivo protocol:
1. Khan OF, Kowalski PS, Doloff JC, Tsosie JK, Bakthavatchalu V, Winn CB, Haupt J, Jamiel M, Langer R, Anderson DG. Endothelial siRNA delivery in nonhuman primates using ionizable low-molecular weight polymeric nanoparticles. Sci Adv. 2018 Jun 27;4(6):eaar8409. doi: 10.1126/sciadv.aar8409. PMID: 29963629; PMCID: PMC6021147.
Dahlman JE, Barnes C, Khan O, Thiriot A, Jhunjunwala S, Shaw TE, Xing Y, Sager HB, Sahay G, Speciner L, Bader A, Bogorad RL, Yin H, Racie T, Dong Y, Jiang S, Seedorf D, Dave A, Sandu KS, Webber MJ, Novobrantseva T, Ruda VM, Lytton-Jean AKR, Levins CG, Kalish B, Mudge DK, Perez M, Abezgauz L, Dutta P, Smith L, Charisse K, Kieran MW, Fitzgerald K, Nahrendorf M, Danino D, Tuder RM, von Andrian UH, Akinc A, Schroeder A, Panigrahy D, Kotelianski V, Langer R, Anderson DG. In vivo endothelial siRNA delivery using polymeric nanoparticles with low molecular weight. Nat Nanotechnol. 2014 Aug;9(8):648-655. doi: 10.1038/nnano.2014.84. Epub 2014 May 11. PMID: 24813696; PMCID: PMC4207430.
