FNIR-Tag-NHS is a near-infrared fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. FNIR-Tag-NHS allows a higher degree of labeling (DOL) with less aggregation than IRDye® 800CW. When conjugated to antibodies, FNIR-Tag-NHS exhibits reduced liver uptake in vivo compared with IRDye® 800CW conjugates. In addition, FNIR-Tag-NHS conjugates are sigificantly brighter than IRDye® 800CW conjugates at similar labeling densities in vivo and in vitro. Its antibody conjugation efficiency is greatest at pH 7.4. Excitation/emission maxima = 766/788 nm; Extinction coefficient = 200,000 M-1cm-1; QY = 0.099.
MedKoo Cat#: 465595
Name: FNIR-Tag-NHS
CAS#: 2365033-54-9
Chemical Formula: C57H80N4O17S2
Exact Mass: 1156.4960
Molecular Weight: 1157.40
Elemental Analysis: C, 59.15; H, 6.97; N, 4.84; O, 23.50; S, 5.54
| Solvent | mg/mL | mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 11.6 | 10.00 | |
| DMF | 11.6 | 10.00 |
The following data is based on the product molecular weight 1,157.40 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
