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MedKoo Cat#: 465302 | Name: CNP-G3
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

CNP-G3 is a colorimetric substrate for α-amylases. Upon hydrolysis by α-amylases, 2-chloro-4-nitrophenol is released which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity. CNP-G3 has been used to characterize the activity of human pancreatic or salivary amylase.

Chemical Structure

CNP-G3
CNP-G3
CAS#118291-90-0

Theoretical Analysis

MedKoo Cat#: 465302

Name: CNP-G3

CAS#: 118291-90-0

Chemical Formula: C24H34ClNO18

Exact Mass: 659.1464

Molecular Weight: 659.98

Elemental Analysis: C, 43.68; H, 5.19; Cl, 5.37; N, 2.12; O, 43.64

Price and Availability

Size Price Availability Quantity
100mg USD 350.00 2 Weeks
250mg USD 650.00 2 Weeks
1g USD 1,250.00 2 Weeks
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Related CAS #
No Data
Synonym
CNP-G3; CNPG3; CNP G3; 2-Chloro-4-nitrophenyl α-D-maltotrioside; G3-CNP
IUPAC/Chemical Name
(2R,3R,4S,5S,6R)-2-(((2R,3S,4R,5R,6R)-6-(((2R,3S,4R,5R,6R)-6-(2-chloro-4-nitrophenoxy)-4,5-dihydroxy-2-(hydroxymethyl)tetrahydro-2H-pyran-3-yl)oxy)-4,5-dihydroxy-2-(hydroxymethyl)tetrahydro-2H-pyran-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
InChi Key
KMYYNUOXSFGLNX-XFNLHOCBSA-N
InChi Code
InChI=1S/C24H34ClNO18/c25-8-3-7(26(37)38)1-2-9(8)39-22-18(35)15(32)20(11(5-28)41-22)44-24-19(36)16(33)21(12(6-29)42-24)43-23-17(34)14(31)13(30)10(4-27)40-23/h1-3,10-24,27-36H,4-6H2/t10-,11-,12-,13-,14+,15-,16-,17-,18-,19-,20-,21-,22+,23-,24-/m1/s1
SMILES Code
OC[C@H]([C@H]1O[C@H]2O[C@H](CO)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)O[C@H](OC4=C(Cl)C=C([N+]([O-])=O)C=C4)[C@H](O)[C@H]1O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
To be determined
Shelf Life
>2 years if stored properly
Drug Formulation
To be determined
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Solvent mg/mL mM comments
Solubility
DMF 30.0 45.46
DMSO 30.0 45.46
PBS (pH 7.2) 10.0 15.15
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 659.98 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Tsyrulneva I, Alagappan P, Liedberg B. Colorimetric Detection of Salivary α-Amylase Using Maltose as a Noncompetitive Inhibitor for Polysaccharide Cleavage. ACS Sens. 2019 Apr 26;4(4):865-873. doi: 10.1021/acssensors.8b01343. Epub 2019 Apr 3. PMID: 30895774. 2: Lorentz K. An ideal substrate for the measurement of pancreatic amylase? Clin Chem Lab Med. 2002 Aug;40(8):781-5. doi: 10.1515/CCLM.2002.134. PMID: 12392304. 3: Deutch CE. Characterization of a salt-tolerant extracellular a-amylase from Bacillus dipsosauri. Lett Appl Microbiol. 2002;35(1):78-84. doi: 10.1046/j.1472-765x.2002.01142.x. PMID: 12081555. 4: Gottschalk TE, Tull D, Aghajari N, Haser R, Svensson B. Specificity modulation of barley alpha-amylase through biased random mutagenesis involving a conserved tripeptide in beta --> alpha loop 7 of the catalytic (beta/alpha)(8)-barrel domain. Biochemistry. 2001 Oct 30;40(43):12844-54. doi: 10.1021/bi0108608. PMID: 11669621. 5: Gella FJ, Gubern G, Vidal R, Canalias F. Determination of total and pancreatic alpha-amylase in human serum with 2-chloro-4-nitrophenyl-alpha-D- maltotrioside as substrate. Clin Chim Acta. 1997 Mar 18;259(1-2):147-60. doi: 10.1016/s0009-8981(96)06481-9. PMID: 9086302.