Fisetin-d5 is intended for use as an internal standard for the quantification of fisetin by GC- or LC-MS. Fisetin is a flavonoid that has been found in various fruits and vegetables and has diverse biological activities. It scavenges radicals in a Trolox equivalent antioxidant capacity (TEAC) assay and inhibits oxidative burst induced by N-Formyl-Met-Leu-Phe (fMLP) in isolated human polymorphonuclear neutrophils (PMNs) when used at a concentration of 10 µM. Fisetin (10-60 µM) induces cell cycle arrest at the G1 phase and apoptosis in, as well as inhibits the proliferation of, LNCaP prostate cancer cells. It inhibits LPS-induced production of nitrite, prostaglandin E2 (PGE2), and NF-κB activation in RAW 264.7 cells. Fisetin (1 mg/animal) reduces tumor growth in a CWR22Rυ1 prostate cancer mouse xenograft model.
MedKoo Cat#: 465278
Name: Fisetin-d5
CAS#: 2909407-29-8
Chemical Formula: C15H5D5O6
Exact Mass: 291.0791
Molecular Weight: 291.27
Elemental Analysis: C, 61.86; H, 5.19; O, 32.96
| Solvent | mg/mL | mM | |
|---|---|---|---|
| Solubility | |||
| DMF | 30.0 | 103.00 | |
| DMSO | 30.0 | 103.00 | |
| DMSO:PBS (pH 7.2) (1:1) | 0.5 | 1.72 | |
| Ethanol | 5.0 | 17.17 |
The following data is based on the product molecular weight 291.27 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
