NEM | CAS#128-53-0 | Sulfhydryl group modifier

MedKoo Cat#: 464946 | Name: NEM

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

NEM is a modifier of sulfhydryl groups in proteins. It has commonly been used to inactivate cysteine-containing enzymes in vitro. NEM inhibits prolyl endopeptidase with an IC50 value of 6.3 µM.3 It alkylates cysteine 519 (Cys519) in voltage-gated potassium channel 7.4 (Kv7.4) and activates Kv7.2 , Kv7.4, and Kv7.5, but not Kv7.3, in CHO cells expressing the human channels when used at a concentration of 50 µM.

Chemical Structure

NEM

CAS#128-53-0

Theoretical Analysis

MedKoo Cat#: 464946

Name: NEM

CAS#: 128-53-0

Chemical Formula: C6H7NO2

Exact Mass: 125.0477

Molecular Weight: 125.13

Elemental Analysis: C, 57.59; H, 5.64; N, 11.19; O, 25.57

Price and Availability

Size	Price	Availability
5g	USD 220.00	2 Weeks
10g	USD 360.00	2 Weeks
25g	USD 590.00	2 Weeks
50g	USD 900.00	2 Weeks

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Related CAS #

No Data

Synonym

NEM; NSC 7638; NSC7638; NSC-7638; N-Ethylmaleimide; N Ethylmaleimide;

IUPAC/Chemical Name

1-ethyl-1H-pyrrole-2,5-dione

InChi Key

HDFGOPSGAURCEO-UHFFFAOYSA-N

InChi Code

InChI=1S/C6H7NO2/c1-2-7-5(8)3-4-6(7)9/h3-4H,2H2,1H3

SMILES Code

O=C1C=CC(N1CC)=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

To be determined

Shelf Life

>2 years if stored properly

Drug Formulation

To be determined

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Instruction

View handling instruction

Preparing Stock Solutions

The following data is based on the product molecular weight 125.13 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

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Functional evidence of distinct electrophile-induced activation states of the ion channel TRPA1. Biochem Biophys Rep. 2021 Jun 15;27:101044. doi: 10.1016/j.bbrep.2021.101044. PMID: 34189279; PMCID: PMC8219651. 5: Sun X, Wang X, Zhou HC, Zheng J, Su YX, Luo F. β3-adrenoceptor activation exhibits a dual effect on behaviors and glutamate receptor function in the prefrontal cortex. Behav Brain Res. 2021 Aug 27;412:113417. doi: 10.1016/j.bbr.2021.113417. Epub 2021 Jun 19. PMID: 34157371. 6: Giustarini D, Milzani A, Dalle-Donne I, Rossi R. Measurement of S-glutathionylated proteins by HPLC. Amino Acids. 2021 Jun 15. doi: 10.1007/s00726-021-03015-6. Epub ahead of print. PMID: 34129091. 7: Gorshtein G, Grafinger O, Coppolino MG. Targeting SNARE-Mediated Vesicle Transport to Block Invadopodium-Based Cancer Cell Invasion. Front Oncol. 2021 May 21;11:679955. doi: 10.3389/fonc.2021.679955. PMID: 34094984; PMCID: PMC8177742. 8: Tomin T, Bordag N, Zügner E, Al-Baghdadi A, Schinagl M, Birner-Gruenberger R, Schittmayer M. Blood Plasma Quality Control by Plasma Glutathione Status. Antioxidants (Basel). 2021 May 27;10(6):864. doi: 10.3390/antiox10060864. PMID: 34072235; PMCID: PMC8226592. 9: Martens MC, Edelkamp J, Seebode C, Schäfer M, Stählke S, Krohn S, Jung O, Murua Escobar H, Emmert S, Boeckmann L. Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts. Int J Mol Sci. 2021 May 18;22(10):5293. doi: 10.3390/ijms22105293. PMID: 34069872; PMCID: PMC8157373. 10: Wang P, Wei M, Zhu X, Liu Y, Yoshimura K, Zheng M, Liu G, Kume S, Morishima M, Kurokawa T, Ono K. Nitric oxide down-regulates voltage-gated Na+ channel in cardiomyocytes possibly through S-nitrosylation-mediated signaling. Sci Rep. 2021 May 28;11(1):11273. doi: 10.1038/s41598-021-90840-0. PMID: 34050231; PMCID: PMC8163867. 11: Kim C, Shon MJ, Kim SH, Eun GS, Ryu JK, Hyeon C, Jahn R, Yoon TY. Extreme parsimony in ATP consumption by 20S complexes in the global disassembly of single SNARE complexes. Nat Commun. 2021 May 28;12(1):3206. doi: 10.1038/s41467-021-23530-0. PMID: 34050166; PMCID: PMC8163800. 12: Zhang L, Qin Z, Sharmin F, Lin W, Ricke KM, Zasloff MA, Stewart AFR, Chen HH. Tyrosine phosphatase PTP1B impairs presynaptic NMDA receptor-mediated plasticity in a mouse model of Alzheimer's disease. Neurobiol Dis. 2021 Aug;156:105402. doi: 10.1016/j.nbd.2021.105402. Epub 2021 May 24. PMID: 34044147. 13: Davis LJ, Bright NA, Edgar JR, Parkinson MDJ, Wartosch L, Mantell J, Peden AA, Luzio JP. Organelle tethering, pore formation and SNARE compensation in the late endocytic pathway. J Cell Sci. 2021 May 15;134(10):jcs255463. doi: 10.1242/jcs.255463. Epub 2021 May 27. PMID: 34042162; PMCID: PMC8186482. 14: Moro A, van Nifterick A, Toonen RF, Verhage M. Dynamin controls neuropeptide secretion by organizing dense-core vesicle fusion sites. Sci Adv. 2021 May 21;7(21):eabf0659. doi: 10.1126/sciadv.abf0659. PMID: 34020952; PMCID: PMC8139595. 15: Qian L, Yang X, Li S, Zhao H, Gao Y, Zhao S, Lv X, Zhang X, Li L, Zhai L, Zhou F, Chen B. Reduced O-GlcNAcylation of SNAP-23 promotes cisplatin resistance by inducing exosome secretion in ovarian cancer. Cell Death Discov. 2021 May 18;7(1):112. doi: 10.1038/s41420-021-00489-x. PMID: 34001861; PMCID: PMC8128872. 16: Pulukool SK, Bhagavatham SKS, Kannan V, Sukumar P, Dandamudi RB, Ghaisas S, Kunchala H, Saieesh D, Naik AA, Pargaonkar A, Sharma A, Sivaramakrishnan V. Elevated dimethylarginine, ATP, cytokines, metabolic remodeling involving tryptophan metabolism and potential microglial inflammation characterize primary open angle glaucoma. Sci Rep. 2021 May 7;11(1):9766. doi: 10.1038/s41598-021-89137-z. PMID: 33963197; PMCID: PMC8105335. 17: Young KZ, Cartee NMP, Lee SJ, Keep SG, Ivanova MI, Wang MM. Electrophilic and Drug-Induced Stimulation of NOTCH3 N-terminal Fragment Oligomerization in Cerebrovascular Pathology. Transl Stroke Res. 2021 May 3. doi: 10.1007/s12975-021-00908-2. Epub ahead of print. PMID: 33939102. 18: Ivanova D, Dobson KL, Gajbhiye A, Davenport EC, Hacker D, Ultanir SK, Trost M, Cousin MA. Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes. Sci Adv. 2021 Apr 30;7(18):eabf3873. doi: 10.1126/sciadv.abf3873. PMID: 33931449; PMCID: PMC8087399. 19: Edelmann MR, Muser T. Tritium O-Methylation of N-Alkoxy Maleimide Derivatives as Labeling Reagents for Biomolecules. Bioconjug Chem. 2021 May 19;32(5):1027-1033. doi: 10.1021/acs.bioconjchem.1c00202. Epub 2021 Apr 28. PMID: 33909418. 20: Liu F, Li JP, Li LS, Liu Q, Li SW, Song ML, Li S, Zhang Y. The canonical α-SNAP is essential for gametophytic development in Arabidopsis. PLoS Genet. 2021 Apr 22;17(4):e1009505. doi: 10.1371/journal.pgen.1009505. PMID: 33886546; PMCID: PMC8096068.