3-IP sodium salt | CAS#3318-43-2 | Alkaline phosphatase substrate

MedKoo Cat#: 464813 | Name: 3-IP sodium salt

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

3-Indoxyl phosphate (3-IP) is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate has been used in ELISAs.

Chemical Structure

3-IP sodium salt

CAS#3318-43-2 (sodium)

Theoretical Analysis

MedKoo Cat#: 464813

Name: 3-IP sodium salt

CAS#: 3318-43-2 (sodium)

Chemical Formula: C8H6NNa2O4P

Exact Mass: 0.0000

Molecular Weight: 257.09

Elemental Analysis: C, 37.37; H, 2.35; N, 5.45; Na, 17.88; O, 24.89; P, 12.05

Price and Availability

Size	Price	Availability	Quantity
250mg	USD 385.00	2 Weeks
1g	USD 950.00	2 Weeks

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Synonym

3-IP sodium salt; 3IP sodium salt; 3 IP sodium salt; 3-Indoxyl Phosphate sodium salt; 3 Indoxyl Phosphate sodium salt; 3Indoxyl Phosphate sodium salt;

IUPAC/Chemical Name

sodium 1H-indol-3-yl phosphate

InChi Key

WCSKWBKPKXJWEG-UHFFFAOYSA-L

InChi Code

InChI=1S/C8H8NO4P.2Na/c10-14(11,12)13-8-5-9-7-4-2-1-3-6(7)8;;/h1-5,9H,(H2,10,11,12);;/q;2*+1/p-2

SMILES Code

O=P(OC1=CNC2=C1C=CC=C2)([O-])[O-].[Na+].[Na+]

Appearance

Solid powder

Purity

>95% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

To be determined

Shelf Life

>2 years if stored properly

Drug Formulation

To be determined

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Instruction

View handling instruction

	Solvent	mg/mL	mM
Solubility
	PBS (pH 7.2)	10.0	38.90

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 257.09 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

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