Nalidixate sodium anhydrous | CAS# 3374-05-8 | Inhibitor of the A subunit of bacterial DNA GYRASE

MedKoo Cat#: 413853 | Name: Nalidixate sodium anhydrous

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Nalidixate sodium anhydrous is a synthetic 1,8-naphthyridine antimicrobial agent with a limited bacteriocidal spectrum. It is an inhibitor of the A subunit of bacterial DNA GYRASE.

Chemical Structure

Nalidixate sodium anhydrous

CAS#3374-05-8

Theoretical Analysis

MedKoo Cat#: 413853

Name: Nalidixate sodium anhydrous

CAS#: 3374-05-8

Chemical Formula: C12H11N2NaO3

Exact Mass: 254.0667

Molecular Weight: 254.22

Elemental Analysis: C, 56.70; H, 4.36; N, 11.02; Na, 9.04; O, 18.88

Price and Availability

Size	Price	Availability
1g	USD 202.00	2 Weeks
5g	USD 353.00	2 Weeks
25g	USD 663.00	2 Weeks
100g	USD 1,288.00	2 Weeks

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Related CAS #

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Synonym

Nalidixate sodium anhydrous; Win18320; Win-18320; Win 18320

IUPAC/Chemical Name

1,8-Naphthyridine-3-carboxylic acid, 1-ethyl-1,4-dihydro-7-methyl-4-oxo-, sodium salt

InChi Key

ROKRAUFZFDQWLE-UHFFFAOYSA-M

InChi Code

InChI=1S/C12H12N2O3.Na/c1-3-14-6-9(12(16)17)10(15)8-5-4-7(2)13-11(8)14;/h4-6H,3H2,1-2H3,(H,16,17);/q;+1/p-1

SMILES Code

O=C(C1=CN(CC)C2=NC(C)=CC=C2C1=O)[O-].[Na+]

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

To be determined

Shelf Life

>2 years if stored properly

Drug Formulation

To be determined

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

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Product Data

Product Data

Instruction

View handling instruction

Biological target:

Nalidixic acid sodium salt, a quinolone antibiotic, is effective against both gram-positive and gram-negative bacteria.

In vitro activity:

Sodium nalidixate inhibited the cell growth and division of several respiratory competent strains of Saccharomyces cerevisiae. Reference: Mol Gen Genet. 1976 Jul 5;146(1):95-100. https://pubmed.ncbi.nlm.nih.gov/785214/

In vivo activity:

Nalidixic acid preferentially suppressed growth in soft agar of transformed Balb/3T3 mouse cells induced by methylcholanthrene. Nalidixic acid suppressed growth in soft agar of NIH/3T3 mouse cells transformed by an activated c-Ha-ras, without affecting the amount of ras p21 proteins as detected by an immunoblotting analysis using a monoclonal antibody. Reference: Br J Cancer. 1989 Dec;60(6):880-6. https://pubmed.ncbi.nlm.nih.gov/2690912/

Preparing Stock Solutions

The following data is based on the product molecular weight 254.22 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Chadha J, Khullar L. Subinhibitory concentrations of nalidixic acid alter bacterial physiology and induce anthropogenic resistance in a commensal strain of Escherichia coli in vitro. Lett Appl Microbiol. 2021 Nov;73(5):623-633. doi: 10.1111/lam.13550. Epub 2021 Aug 24. PMID: 34376018. 2. Carnevali F, Sarcoe LE, Whittaker PA. Differential effects of nalidixate on the cell growth of respiratory competent strains and cytoplasmic petite mutants of Saccharomyces cerevisiae. Mol Gen Genet. 1976 Jul 5;146(1):95-100. doi: 10.1007/BF00267988. PMID: 785214. 3. Kaneko M, Horikoshi J. Reversible suppression by nalidixic acid of anchorage-independent growth of mouse cells transformed by 3-methylcholanthrene or an activated c-Ha-ras gene. Br J Cancer. 1989 Dec;60(6):880-6. doi: 10.1038/bjc.1989.384. PMID: 2690912; PMCID: PMC2247252. 4. McQueen CA, Rosado RR, Williams GM. Effect of nalidixic acid on DNA repair in rat hepatocytes. Cell Biol Toxicol. 1989 Jun;5(2):201-6. doi: 10.1007/BF00122653. PMID: 2504447.

In vitro protocol:

In vivo protocol:

1. Kaneko M, Horikoshi J. Reversible suppression by nalidixic acid of anchorage-independent growth of mouse cells transformed by 3-methylcholanthrene or an activated c-Ha-ras gene. Br J Cancer. 1989 Dec;60(6):880-6. doi: 10.1038/bjc.1989.384. PMID: 2690912; PMCID: PMC2247252. 2. McQueen CA, Rosado RR, Williams GM. Effect of nalidixic acid on DNA repair in rat hepatocytes. Cell Biol Toxicol. 1989 Jun;5(2):201-6. doi: 10.1007/BF00122653. PMID: 2504447.

1: Callao V, Ramos A. Actividad antibacteriana del ácido nalidíxico (Win 18320) frente a diversas estirpes de gram-negativos [Antibacterial activity of nalidixic acid (WIN 18320) on various strains of Gram-negative organisms]. Microbiol Esp. 1966 Apr-Jun;19(2):173-82. Spanish. PMID: 5974262. 2: TERRERI G, FORSTER RF. ESTUDO COMPARATIVO E OBSERVA C OES CL'INICAS DO WIN-18.320 ('ACIDO NALID'IXICO) EM INFEC C OES DO TRATO URIN'ARIO [COMPARATIVE STUDY AND CLINICAL OBSERVATIONS ON WIN-18320 (NALIDIXIC ACID) IN URINARY TRACT INFECTIONS]. Folha Med. 1964 May;48:283-7. Portuguese. PMID: 14271217.