JF 526 is a fluorogenic yellow fluorescent dye. It is supplied as an NHS ester for coupling to primary amine groups. NHS esters can be converted to relevant substrates for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. It is suitable for confocal fluorescent imaging and super-resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. NHS esters can be multiplexed for two color imaging with Janelia Fluor® 635 SE. It is cell permeable and has an excitation maximum = 526 nm, emission maximum = 550 nm, quantum yield = 0.87, and extinction coefficient = 118,000 M-1cm-1.
MedKoo Cat#: 464513
Name: JF 526
CAS#: 2376841-30-2
Chemical Formula: C31H19F6N3O7
Exact Mass: 659.1127
Molecular Weight: 659.50
Elemental Analysis: C, 56.46; H, 2.90; F, 17.28; N, 6.37; O, 16.98
| Solvent | mg/mL | mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 13.2 | 20.00 |
The following data is based on the product molecular weight 659.50 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
