NSC 5302 is a synthetic coumarin with diverse biological activities. It scavenges alkylperoxy radicals and hypochlorous acid (HOCl; IC50 = 2.85 µM) in cell-free assays, as well as inhibits lipid peroxidation in rat liver microsomes, A23187-induced thromboxane B2 (TXB2) generation in isolated rat peritoneal leukocytes, and myeloperoxidase (MPO) activity in a cell-free assay (IC50s = 12, 1, and 1.06 µM, respectively). NSC 5302 also inhibits rat lens aldose reductase (IC50 = 17 µM) and human carbonic anhydrase I (CAI), CAIX, and CAXII with Ki values of 8.4, 0.19, and 6.4 µM, respectively. It inhibits platelet aggregation induced by arachidonic acid with an IC50 value of 45 µM. NSC 5302 inhibits hepatitis C virus (HCV) non-structural protein 5B (NS5B) polymerase (IC50 = 47.2 µM). It is active against H. pylori with an MIC value of 10 µg/ml.
MedKoo Cat#: 464501
Name: NSC 5302
CAS#: 2107-76-8
Chemical Formula: C10H8O4
Exact Mass: 192.0423
Molecular Weight: 192.17
Elemental Analysis: C, 62.50; H, 4.20; O, 33.30
| Solvent | mg/mL | mM | |
|---|---|---|---|
| Solubility | |||
| DMF | 30.0 | 156.00 | |
| DMSO | 30.0 | 156.00 | |
| DMSO:PBS (pH 7.2) (1:3) | 0.3 | 1.30 | |
| Ethanol | 20.0 | 104.00 |
The following data is based on the product molecular weight 192.17 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
