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MedKoo Cat#: 464325 | Name: HEPES
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

HEPES is a zwitterionic sulfonic acid buffering agent; one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture.

Chemical Structure

HEPES
HEPES
CAS#7365-45-9

Theoretical Analysis

MedKoo Cat#: 464325

Name: HEPES

CAS#: 7365-45-9

Chemical Formula: C8H18N2O4S

Exact Mass: 238.0987

Molecular Weight: 238.30

Elemental Analysis: C, 40.32; H, 7.61; N, 11.76; O, 26.85; S, 13.45

Price and Availability

Size Price Availability Quantity
5g USD 250.00 2 Weeks
25g USD 450.00 2 Weeks
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Related CAS #
No Data
Synonym
HEPES; NSC 166663; NSC166663; NSC-166663;
IUPAC/Chemical Name
2-(4-(2-hydroxyethyl)piperazin-1-yl)ethane-1-sulfonic acid
InChi Key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
InChi Code
InChI=1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
SMILES Code
OCCN1CCN(CC1)CCS(=O)(O)=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
To be determined
Shelf Life
>2 years if stored properly
Drug Formulation
To be determined
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
HEPES, a nonvolatile zwitterionic chemical buffering agent, is broadly applied in cell culture. HEPES is effective at pH 6.8 to 8.2. HEPES is also a potent inducer of lysosome biogenesis.
In vitro activity:
HEPES-buffered medium markedly influences processing of GCase, its lysosomal degradation, and the total cellular enzyme level. HEPES-containing medium was also found to reduce maturation of other lysosomal enzymes (α-glucosidase and β-glucuronidase) in cells. The presence of HEPES in bicarbonate containing medium increases GCase activity in GD-patient derived fibroblasts, illustrating how the supplementation of HEPES complicates the use of cultured cells for diagnosing GD. Reference: J Cell Biochem. 2022 May;123(5):893-905. https://pubmed.ncbi.nlm.nih.gov/35312102/
In vivo activity:
Among stored groups, improved pH regulation (by adding HEPES) and increased metabolism (by adding pyruvate) appeared to correlate with cellular survival. Lungs stored in Perfadex with bicarbonate buffer alone had significantly greater cell death than all other groups. Improved buffer capacity (Perfadex/HEPES Group) or the addition of pyruvate (Pyruvate Group) improved lung preservation to a similar degree compared to Perfadex alone (p<.05). The least cell death among stored lungs was encountered when allografts were stored in pyruvate and HEPES supplemented preservation solution. Reference: Transplant Proc. 2010 Sep;42(7):2771-6. https://pubmed.ncbi.nlm.nih.gov/20832585/
Solvent mg/mL mM
Solubility
Water 178.8 750.17
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 238.30 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Wang X, Wang Y, Yin L, Zhang Q, Wang S. Surfactant-free synthesis of fluorescent platinum nanoclusters using HEPES buffer for hypochlorous acid sensing and imaging. RSC Adv. 2022 Apr 4;12(17):10395-10400. doi: 10.1039/d1ra09064j. PMID: 35424968; PMCID: PMC8978884. 2. van der Lienden MJC, Aten J, Boot RG, van Eijk M, Aerts JMFG, Kuo CL. HEPES-buffering of bicarbonate-containing culture medium perturbs lysosomal glucocerebrosidase activity. J Cell Biochem. 2022 May;123(5):893-905. doi: 10.1002/jcb.30234. Epub 2022 Mar 21. PMID: 35312102; PMCID: PMC9314694. 3. Peltz M, Milchgrub S, Jessen ME, Meyer DM. Effect of pyruvate and HEPES on rat lung allograft acidosis and cell death after long-term hypothermic storage. Transplant Proc. 2010 Sep;42(7):2771-6. doi: 10.1016/j.transproceed.2010.06.004. PMID: 20832585; PMCID: PMC2954414. 4. Desaphy JF, Joffre M. Inhibitory effect of internal sodium and Hepes on the voltage-dependent potassium conductance of rat Leydig cells. Biochim Biophys Acta. 1996 Nov 13;1285(1):9-13. doi: 10.1016/s0005-2736(96)00133-2. PMID: 8948469.
In vitro protocol:
1. Wang X, Wang Y, Yin L, Zhang Q, Wang S. Surfactant-free synthesis of fluorescent platinum nanoclusters using HEPES buffer for hypochlorous acid sensing and imaging. RSC Adv. 2022 Apr 4;12(17):10395-10400. doi: 10.1039/d1ra09064j. PMID: 35424968; PMCID: PMC8978884. 2. van der Lienden MJC, Aten J, Boot RG, van Eijk M, Aerts JMFG, Kuo CL. HEPES-buffering of bicarbonate-containing culture medium perturbs lysosomal glucocerebrosidase activity. J Cell Biochem. 2022 May;123(5):893-905. doi: 10.1002/jcb.30234. Epub 2022 Mar 21. PMID: 35312102; PMCID: PMC9314694.
In vivo protocol:
1. Peltz M, Milchgrub S, Jessen ME, Meyer DM. Effect of pyruvate and HEPES on rat lung allograft acidosis and cell death after long-term hypothermic storage. Transplant Proc. 2010 Sep;42(7):2771-6. doi: 10.1016/j.transproceed.2010.06.004. PMID: 20832585; PMCID: PMC2954414. 2. Desaphy JF, Joffre M. Inhibitory effect of internal sodium and Hepes on the voltage-dependent potassium conductance of rat Leydig cells. Biochim Biophys Acta. 1996 Nov 13;1285(1):9-13. doi: 10.1016/s0005-2736(96)00133-2. PMID: 8948469.
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