MedKoo Biosciences, Inc.
Tel:   +1-919-636-5577    Fax:   +1-919-980-4831    Email:   sales@medkoo.com
Search
Login Register
Search
MedKoo Cat#: 464222 | Name: Methylene Green

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Methylene green is an organic chloride salt having 3,7-bis(dimethylamino)-4-nitrophenothiazin-5-ium as the counterion. It stains nuclei green and is sometimes used as a counterstain to red or purple primary stains. It has a role as a fluorochrome and a histological dye. It contains a 3,7-bis(dimethylamino)-4-nitrophenothiazin-5-ium.

Chemical Structure

Methylene Green
Methylene Green
CAS#2679-01-8

Theoretical Analysis

MedKoo Cat#: 464222

Name: Methylene Green

CAS#: 2679-01-8

Chemical Formula: C16H17ClN4O2S

Exact Mass: 364.0761

Molecular Weight: 364.85

Elemental Analysis: C, 52.67; H, 4.70; Cl, 9.72; N, 15.36; O, 8.77; S, 8.79

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.
Bulk Inquiry
Related CAS #
No Data
Synonym
Methylene Green; NSC 367083; NSC367083; NSC-367083; NSC 9403; NSC9403; NSC-9403; C.I. Basic Green 5; Basic Green 5;
IUPAC/Chemical Name
3,7-bis(dimethylamino)-4-nitrophenothiazin-5-ium chloride
InChi Key
YYGBVRCTHASBKD-UHFFFAOYSA-M
InChi Code
InChI=1S/C16H17N4O2S.ClH/c1-18(2)10-5-6-11-14(9-10)23-16-12(17-11)7-8-13(19(3)4)15(16)20(21)22;/h5-9H,1-4H3;1H/q+1;/p-1
SMILES Code
CN(c(cc1)cc2c1nc(ccc(N(C)C)c3[N+]([O-])=O)c3[s+]2)C.[Cl-]
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
To be determined
Shelf Life
>2 years if stored properly
Drug Formulation
To be determined
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Solvent mg/mL mM comments
Solubility
To be determined 0.0 0.00
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 364.85 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Pinyou P, Conzuelo F, Sliozberg K, Vivekananthan J, Contin A, Pöller S, Plumeré N, Schuhmann W. Coupling of an enzymatic biofuel cell to an electrochemical cell for self-powered glucose sensing with optical readout. Bioelectrochemistry. 2015 Dec;106(Pt A):22-7. doi: 10.1016/j.bioelechem.2015.04.003. Epub 2015 Apr 9. PMID: 25892686. 2: Evtugyn GA, Stepanova VB, Porfireva AV, Zamaleeva AI, Fakhrullin RR. Electrochemical DNA sensors based on nanostructured organic dyes/DNA/polyelectrolyte complexes. J Nanosci Nanotechnol. 2014 Sep;14(9):6738-47. doi: 10.1166/jnn.2014.9345. PMID: 25924325. 3: Regeta K, Bannwarth C, Grimme S, Allan M. Free electrons and ionic liquids: study of excited states by means of electron-energy loss spectroscopy and the density functional theory multireference configuration interaction method. Phys Chem Chem Phys. 2015 Jun 28;17(24):15771-80. doi: 10.1039/c5cp01417d. Epub 2015 May 28. PMID: 26018044. 4: Tran HN, You SJ, Chao HP. Fast and efficient adsorption of methylene green 5 on activated carbon prepared from new chemical activation method. J Environ Manage. 2017 Mar 1;188:322-336. doi: 10.1016/j.jenvman.2016.12.003. Epub 2016 Dec 19. PMID: 28006742. 5: Jeon SJ, Lim SJ. Purification and Characterization of the Laccase Involved in Dye Decolorization by the White-Rot Fungus Marasmius scorodonius. J Microbiol Biotechnol. 2017 Jun 28;27(6):1120-1127. doi: 10.4014/jmb.1701.01004. PMID: 28376610. 6: Tran HN, Lee CK, Nguyen TV, Chao HP. Saccharide-derived microporous spherical biochar prepared from hydrothermal carbonization and different pyrolysis temperatures: synthesis, characterization, and application in water treatment. Environ Technol. 2018 Nov;39(21):2747-2760. doi: 10.1080/09593330.2017.1365941. Epub 2017 Aug 24. PMID: 28791934. 7: Lee D, Lee S, Rho J, Jang W, Han SH, Chung TD. 3D interdigitated electrode array in the microchannel free of reference and counter electrodes. Biosens Bioelectron. 2018 Mar 15;101:317-321. doi: 10.1016/j.bios.2017.09.050. Epub 2017 Sep 29. PMID: 29033058. 8: Tran HN, Wen YC, Wang YF, You SJ. Highly efficient removal of hazardous aromatic pollutants by micro-nano spherical carbons synthesized from different chemical activation methods: a comparison study. Environ Technol. 2019 Apr;40(11):1376-1391. doi: 10.1080/09593330.2017.1422551. Epub 2018 Jan 16. PMID: 29283319. 9: Kausar S, Khan W. Comparative Efficacy of Diethylcarbamazine, Nitazoxanide and Nanocomposite of Nitazoxanide and Silver Nanoparticles on the Dehydrogenases of TCA Cycle in Setaria cervi, in Vitro. Iran J Parasitol. 2018 Jul- Sep;13(3):399-405. PMID: 30483331; PMCID: PMC6243168. 10: Kazakov DV , Nazyrov TI , Safarov FE , Yaremenko IA , Terent'ev AO . Chemiluminescence in the reaction of 1,2,4,5-tetraoxanes with ferrous ions in the presence of xanthene dyes: fundamentals and perspectives of analytical applications. Photochem Photobiol Sci. 2019 May 15;18(5):1130-1137. doi: 10.1039/c8pp00472b. PMID: 30758385. 11: John CW, Proshlyakov DA. Fourier Transform Infrared Spectrovoltammetry and Quantitative Modeling of Analytes in Kinetically Constrained Redox Mixtures. Anal Chem. 2019 Aug 6;91(15):9563-9570. doi: 10.1021/acs.analchem.9b00859. Epub 2019 Jul 12. PMID: 31257856; PMCID: PMC7050292. 12: Jeong MK, Soh HY, Suh HL. Three new species of Heteromastus (Annelida, Capitellidae) from Korean waters, with genetic evidence based on two gene markers. Zookeys. 2019 Aug 5;869:1-18. doi: 10.3897/zookeys.869.34380. PMID: 31413658; PMCID: PMC6690876. 13: Sun X, Zhang H, Hao S, Zhai J, Dong S. A Self-Powered Biosensor with a Flake Electrochromic Display for Electrochemical and Colorimetric Formaldehyde Detection. ACS Sens. 2019 Oct 25;4(10):2631-2637. doi: 10.1021/acssensors.9b00917. Epub 2019 Sep 18. PMID: 31441298. 14: Guo S, Yu P, Li W, Yi Y, Wu F, Mao L. Electron Hopping by Interfacing Semiconducting Graphdiyne Nanosheets and Redox Molecules for Selective Electrocatalysis. J Am Chem Soc. 2020 Jan 29;142(4):2074-2082. doi: 10.1021/jacs.9b13678. Epub 2020 Jan 16. PMID: 31909992. 15: Ramanathan S, Radhika N, Padmanabhan D, Durairaj A, Paul Selvin S, Lydia S, Kavitha S, Vasanthkumar S. Eco-friendly Synthesis of CRGO and CRGO/SnO2 Nanocomposite for Photocatalytic Degradation of Methylene Green Dye. ACS Omega. 2019 Dec 24;5(1):158-169. doi: 10.1021/acsomega.9b02281. PMID: 31956762; PMCID: PMC6963968. 16: Tsuruoka N, Soto SS, Tahar AB, Zebda A, Tsujimura S. Mediated electrochemical oxidation of glucose via poly(methylene green) grafted on the carbon surface catalyzed by flavin adenine dinucleotide-dependent glucose dehydrogenase. Colloids Surf B Biointerfaces. 2020 Apr 21;192:111065. doi: 10.1016/j.colsurfb.2020.111065. Epub ahead of print. PMID: 32361503. 17: Gerstenbrand R, Fülöp D, Samu F, Tholt G. Simplified and rapid staining of leafhopper salivary sheaths in plant tissues for electrical penetration graph waveform correlations. Micron. 2021 Jan;140:102959. doi: 10.1016/j.micron.2020.102959. Epub 2020 Oct 10. PMID: 33120163. 18: Sun X, Zhang H, Huang L, Hao S, Zhai J, Dong S. A naked-eye readout self- powered electrochemical biosensor toward indoor formaldehyde: On-site detection and exposure risk warning. Biosens Bioelectron. 2021 Apr 1;177:112975. doi: 10.1016/j.bios.2021.112975. Epub 2021 Jan 6. PMID: 33444898. 19: Liang H, Wang S, Lu Y, Ren P, Li G, Yang F, Chen Y. Highly efficient and cheap treatment of dye by graphene-doped TiO2 microspheres. Water Sci Technol. 2021 Jan;83(1):223-232. doi: 10.2166/wst.2020.545. PMID: 33460420.