MedKoo Biosciences, Inc.
Tel:   +1-919-636-5577    Fax:   +1-919-980-4831    Email:   sales@medkoo.com
Search
Login Register
Search
MedKoo Cat#: 464216 | Name: NAI-N3
Featured New

Description:

WARNING: This product is for research use only, not for human or veterinary use.

NAI-N3 is a probe for icSHAPE (in vivo click selective 2'-hydroxyl acylation and profiling experiment). It acylates the 2'-OH in ssRNA and enables RNA structure profiling in vivo and ex vivo for all four bases. NAI-N3 can be used for site-specific RNA acylation using the RAIL (RNA Acylation at Induced Loops) method or in Apta-Seq to discover new aptamers in selected pools.

Chemical Structure

NAI-N3
NAI-N3
CAS#1612756-29-2

Theoretical Analysis

MedKoo Cat#: 464216

Name: NAI-N3

CAS#: 1612756-29-2

Chemical Formula: C10H8N6O

Exact Mass: 228.0760

Molecular Weight: 228.22

Elemental Analysis: C, 52.63; H, 3.53; N, 36.83; O, 7.01

Price and Availability

Size Price Availability Quantity
5mg USD 150.00 Ready to ship
10mg USD 250.00 Ready to ship
25mg USD 450.00 Ready to ship
50mg USD 750.00 Ready to ship
100mg USD 1,250.00 Ready to ship
200mg USD 2,150.00 Ready to ship
Show More
Bulk Inquiry
Buy Now
Add to Cart
Related CAS #
No Data
Synonym
NAI-N3; NAIN3; NAI N3;
IUPAC/Chemical Name
(2-(azidomethyl)pyridin-3-yl)(1H-imidazol-1-yl)methanone
InChi Key
QPSQVTFTPHUCEH-UHFFFAOYSA-N
InChi Code
InChI=1S/C10H8N6O/c11-15-14-6-9-8(2-1-3-13-9)10(17)16-5-4-12-7-16/h1-5,7H,6H2
SMILES Code
[N-]=[N+]=NCC1=C(C=CC=N1)C(N2C=CN=C2)=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
To be determined
Shelf Life
>2 years if stored properly
Drug Formulation
to be determined
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Biological target:
NAI-N3 is a RNA acylation reagent that enables RNA purification. NAI-N3 is a dual-function SHAPE (selective 2-hydroxyl acylation and profiling experiment) probe (RNA structure probe and enrichment).
In vitro activity:
In brief, the bifunctional NAI-N3 SHAPE reagent is used to label all RNAs inside living cells (or in vitro refolded RNA) to generate covalent modifications on targeted RNAs at single-nucleotide resolution. Reference: Nat Protoc. 2016 Feb;11(2):273-90. https://pubmed.ncbi.nlm.nih.gov/26766114/
In vivo activity:
This study has tested the NAI-N3 reagent in bacterial (Gram-negative Escherichia coli), yeast, insect and mammalian cells for SHAPE experiments. All of these systems proved to be amenable for icSHAPE modification and RNA structure determination. This study has successfully generated icSHAPE data from experiments in which the in vivo modification occurs at temperatures between 30 and 37 °C, to accommodate the specific culture temperatures. Thus, the procedure can be easily adapted to a wide variety of culture systems to allow transcriptome-wide RNA structure determination in most model systems. Reference: Nat Protoc. 2016 Feb;11(2):273-90. https://pubmed.ncbi.nlm.nih.gov/26766114/
Solvent mg/mL mM
Solubility
DMSO 250.0 1,095.46
Ethanol 16.7 73.05
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 228.22 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Flynn RA, Zhang QC, Spitale RC, Lee B, Mumbach MR, Chang HY. Transcriptome-wide interrogation of RNA secondary structure in living cells with icSHAPE. Nat Protoc. 2016 Feb;11(2):273-90. doi: 10.1038/nprot.2016.011. Epub 2016 Jan 14. PMID: 26766114; PMCID: PMC4896316.
In vitro protocol:
1. Flynn RA, Zhang QC, Spitale RC, Lee B, Mumbach MR, Chang HY. Transcriptome-wide interrogation of RNA secondary structure in living cells with icSHAPE. Nat Protoc. 2016 Feb;11(2):273-90. doi: 10.1038/nprot.2016.011. Epub 2016 Jan 14. PMID: 26766114; PMCID: PMC4896316.
In vivo protocol:
1. Flynn RA, Zhang QC, Spitale RC, Lee B, Mumbach MR, Chang HY. Transcriptome-wide interrogation of RNA secondary structure in living cells with icSHAPE. Nat Protoc. 2016 Feb;11(2):273-90. doi: 10.1038/nprot.2016.011. Epub 2016 Jan 14. PMID: 26766114; PMCID: PMC4896316.
1: Flynn RA, Zhang QC, Spitale RC, Lee B, Mumbach MR, Chang HY. Transcriptome- wide interrogation of RNA secondary structure in living cells with icSHAPE. Nat Protoc. 2016 Feb;11(2):273-90. doi: 10.1038/nprot.2016.011. Epub 2016 Jan 14. PMID: 26766114; PMCID: PMC4896316.

View History

Naluzotan hydrochloride

Naluzotan hydrochloride

LXN33404

LXN33404

Gostatin

Gostatin