MedKoo Cat#: 575570 | Name: Fluo-3
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Fluo-3 is a fluorescent Ca(2+) indicator that permits continuous monitoring of Ca without interference with use of UV-sensitive caged compounds.

Chemical Structure

Fluo-3
Fluo-3
CAS#123632-39-3

Theoretical Analysis

MedKoo Cat#: 575570

Name: Fluo-3

CAS#: 123632-39-3

Chemical Formula: C36H30Cl2N2O13

Exact Mass: 768.1125

Molecular Weight: 769.54

Elemental Analysis: C, 56.19; H, 3.93; Cl, 9.21; N, 3.64; O, 27.03

Price and Availability

Size Price Availability Quantity
1mg USD 850.00 2 Weeks
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Related CAS #
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Synonym
Fluo 3; Fluo3; Fluo-3; Fluo-3-AM
IUPAC/Chemical Name
Glycine, N-(2-(2-(2-(bis(carboxymethyl)amino)-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthene-9-yl)phenoxy)ethoxy)-4-methylphenyl)-N-(carboxymethyl)-
InChi Key
OZLGRUXZXMRXGP-UHFFFAOYSA-N
InChi Code
InChI=1S/C36H30Cl2N2O13/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50)
SMILES Code
ClC(C=C1C(C2=CC(OCCOC3=C(C=CC(C)=C3)N(CC(O)=O)CC(O)=O)=C(C=C2)N(CC(O)=O)CC(O)=O)=C4C=C5Cl)=C(O)C=C1OC4=CC5=O
Appearance
Solid powder
Purity
>70% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 769.54 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1. Zhang S, Li C, Gao J, Qiu X, Cui Z. [Application of the Ca2+ indicator fluo-3 and fluo-4 in the process of H2O2 induced apoptosis of A549 cell]. Zhongguo Fei Ai Za Zhi. 2014 Mar;17(3):197-202. Chinese. doi: 10.3779/j.issn.1009-3419.2014.03.03. PMID: 24667255; PMCID: PMC6019359. 2. Timmers AC, Reiss HD, Schel JH. Digitonin-aided loading of Fluo-3 into embryogenic plant cells. Cell Calcium. 1991 Jul;12(7):515-21. doi: 10.1016/0143-4160(91)90033-b. PMID: 1934038. 3. Merritt JE, McCarthy SA, Davies MP, Moores KE. Use of fluo-3 to measure cytosolic Ca2+ in platelets and neutrophils. Loading cells with the dye, calibration of traces, measurements in the presence of plasma, and buffering of cytosolic Ca2+. Biochem J. 1990 Jul 15;269(2):513-9. doi: 10.1042/bj2690513. PMID: 2117443; PMCID: PMC1131607.