Synonym
TCID; UCH-L3 Inhibitor
IUPAC/Chemical Name
4,5,6,7-tetrachloro-1H-indene-1,3(2H)-dione
InChi Key
IDLAOWFFKWRNHB-UHFFFAOYSA-N
InChi Code
InChI=1S/C9H2Cl4O2/c10-6-4-2(14)1-3(15)5(4)7(11)9(13)8(6)12/h1H2
SMILES Code
O=C(CC1=O)C2=C1C(Cl)=C(Cl)C(Cl)=C2Cl
Purity
>95% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Preparing Stock Solutions
The following data is based on the
product
molecular weight
283.91
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1. Mason, D.E., Ek, J., Peters, E.C., et al. Substrate profiling of deubiquitin hydrolases with a positional scanning library and mass spectrometry. Biochemistry 43(21), 6535-6544 (2004).
2. Setsuie, R., Sakurai, M., Sakaguchi, Y., et al. Ubiquitin dimers control the hydrolase activity of UCH-L3. Neurochemistry International 54(5-6), 314-321 (2009).
3. Wada, H., Kito, K., Caskey, L.S., et al. Cleavage of the C-terminus of NEDD8 by UCH-L3. Biochemical and Biophysical Research Communications 251(3), 668-692 (1998).
4. Iphöfer, A., Kummer, A., Nimtz, M., et al. Profiling ubiquitin linkage specificities of deubiquitinating enzymes with branched ubiquitin isopeptide probes. ChemBioChem 13(10), 1416-1420 (2012).
5. Liu, Y., Lashuel, H.A., Choi, S., et al. Discovery of inhibitors that elucidate the role of UCH-L1 activity in the H1299 lung cancer cell line. Chemistry & Biology 10(9), 837-846 (2003).
