Synonym
Aminobenzyl-edta; Aminobenzyl-EDTA;
IUPAC/Chemical Name
2,2',2'',2'''-((3-(4-aminophenyl)propane-1,2-diyl)bis(azanetriyl))tetraacetic acid
InChi Key
RPBJIOJIMOUOIN-UHFFFAOYSA-N
InChi Code
InChI=1S/C17H23N3O8/c18-12-3-1-11(2-4-12)5-13(20(9-16(25)26)10-17(27)28)6-19(7-14(21)22)8-15(23)24/h1-4,13H,5-10,18H2,(H,21,22)(H,23,24)(H,25,26)(H,27,28)
SMILES Code
Nc1ccc(CC(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=O)cc1
Purity
>90% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Preparing Stock Solutions
The following data is based on the
product
molecular weight
397.38
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1: Kong T, Hao XQ, Li XB, Liu GW, Zhang ZG, Yang ZJ, Wang Z, Tang J, Yang W, Sun J. Preparation of novel monoclonal antibodies against chelated cadmium ions. Biol Trace Elem Res. 2013 Apr;152(1):117-24. doi: 10.1007/s12011-013-9599-6. Epub 2013 Feb 2. PMID: 23377609.
2: Zhang Y, Li X, Liu G, Wang Z, Kong T, Tang J, Zhag P, Yang W, Li D, Liu L, Xie G, Wang J. Development of ELISA for detection of mercury based on specific monoclonal antibodies against mercury-chelate. Biol Trace Elem Res. 2011 Dec;144(1-3):854-64. doi: 10.1007/s12011-011-9031-z. Epub 2011 Mar 25. PMID: 21437665.
3: Zhao H, Xue C, Nan T, Tan G, Li Z, Li QX, Zhang Q, Wang B. Detection of copper ions using microcantilever immunosensors and enzyme-linked immunosorbent assay. Anal Chim Acta. 2010 Aug 31;676(1-2):81-6. doi: 10.1016/j.aca.2010.07.041. Epub 2010 Aug 3. PMID: 20800746.
4: Delehanty JB, Jones RM, Bishop TC, Blake DA. Identification of important residues in metal-chelate recognition by monoclonal antibodies. Biochemistry. 2003 Dec 9;42(48):14173-83. doi: 10.1021/bi034839d. PMID: 14640685.
5: Chmura AJ, Orton MS, Meares CF. Antibodies with infinite affinity. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8480-4. doi: 10.1073/pnas.151260298. Epub 2001 Jul 10. PMID: 11447282; PMCID: PMC37461.
6: Huq I, Rana TM. Probing the proximity of the core domain of an HIV-1 Tat fragment in a Tat-TAR complex by affinity cleaving. Biochemistry. 1997 Oct 14;36(41):12592-9. doi: 10.1021/bi971011g. PMID: 9376365.
7: Song AI, Rana TM. Synthesis of an amino acid analogue to incorporate p-aminobenzyl-EDTA in peptides. Bioconjug Chem. 1997 Mar-Apr;8(2):249-52. doi: 10.1021/bc960084u. PMID: 9095368.
8: Studer M, Kroger LA, DeNardo SJ, Kukis DL, Meares CF. Influence of a peptide linker on biodistribution and metabolism of antibody-conjugated benzyl-EDTA. Comparison of enzymatic digestion in vitro and in vivo. Bioconjug Chem. 1992 Sep-Oct;3(5):424-9. doi: 10.1021/bc00017a012. PMID: 1420442.
9: Studer M, Meares CF. A convenient and flexible approach for introducing linkers on bifunctional chelating agents. Bioconjug Chem. 1992 Sep- Oct;3(5):420-3. doi: 10.1021/bc00017a011. PMID: 1420441.
10: Thakur ML, Richard MD, White FW 3rd. Monoclonal antibodies as agents for selective radiolabeling of human neutrophils. J Nucl Med. 1988 Nov;29(11):1817-25. PMID: 3141596.
