NP19 | CAS#2377916-66-8 | PD-1/PD-L1 Interaction inhibitor

MedKoo Cat#: 535178 | Name: NP19

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

NP19 is an inhibitor of the protein-protein interaction between programmed cell death 1 (PD-1) and its ligand PD-L1 (IC50 = 12.5 nM in a homogenous time-resolved fluorescence (HTRF) assay). It increases IFN-γ production by isolated human peripheral blood mononuclear cells (PBMCs) in co-culture with anti-CD3 single-chain variable fragment- and PD-L1-expressing Hep3B hepatoma cells (EC50 = ~3 µM). NP19 (25, 50, and 100 mg/kg) reduces tumor growth in a B16/F10 murine melanoma model and an H22 murine hepatocellular carcinoma model.

Chemical Structure

NP19

CAS#2377916-66-8

Theoretical Analysis

MedKoo Cat#: 535178

Name: NP19

CAS#: 2377916-66-8

Chemical Formula: C33H31ClN2O4

Exact Mass: 554.1972

Molecular Weight: 555.07

Elemental Analysis: C, 71.41; H, 5.63; Cl, 6.39; N, 5.05; O, 11.53

Price and Availability

Size	Price	Availability	Quantity
5mg	USD 750.00	2 Weeks
10mg	USD 1,250.00	2 Weeks

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Related CAS #

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Synonym

NP19; NP-19; NP 19;

IUPAC/Chemical Name

2-((5-Chloro-2-((3-cyanobenzyl)oxy)-4-((2-methyl-[1,1'-biphenyl]-3-yl)methoxy)benzyl)amino)-2-methylpropanoic acid

InChi Key

YZPKZNFXWGTUGO-UHFFFAOYSA-N

InChi Code

InChI=1S/C33H31ClN2O4/c1-22-26(13-8-14-28(22)25-11-5-4-6-12-25)21-40-31-17-30(39-20-24-10-7-9-23(15-24)18-35)27(16-29(31)34)19-36-33(2,3)32(37)38/h4-17,36H,19-21H2,1-3H3,(H,37,38)

SMILES Code

CC(C)(NCC1=CC(Cl)=C(OCC2=C(C)C(C3=CC=CC=C3)=CC=C2)C=C1OCC4=CC=CC(C#N)=C4)C(O)=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

PD-1/PD-L1-IN-NP19 is a PD-1/PD-L1 inhibitor, with an IC50 of 12.5 nM for human PD-1/PD-L1 interaction.

In vitro activity:

Compound NP19 inhibited the human PD-1/PD-L1 interaction with IC50 values of 12.5 nM in homogeneous time-resolved fluorescence (HTRF) binding assays. In addition, NP19 dose-dependently elevated IFN-γ production in a coculture model of Hep3B/OS-8/hPD-L1 and CD3 T cells. Reference: J Med Chem. 2020 Aug 13;63(15):8338-8358. https://pubmed.ncbi.nlm.nih.gov/32667799/

In vivo activity:

Furthermore, NP19 displayed significant in vivo antitumor efficacy in two different mouse models of cancer (a melanoma B16-F10 tumor model and an H22 hepatoma tumor model). Moreover, H&E staining and flow cytometry data suggested that NP19 activated the immune microenvironment in the tumor, which may contribute to its antitumor effects. Reference: J Med Chem. 2020 Aug 13;63(15):8338-8358. https://pubmed.ncbi.nlm.nih.gov/32667799/

	Solvent	mg/mL	mM
Solubility
	DMF	30.0	54.05
	DMSO	16.0	28.83

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 555.07 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

Cheng B, Ren Y, Niu X, Wang W, Wang S, Tu Y, Liu S, Wang J, Yang D, Liao G, Chen J. Discovery of Novel Resorcinol Dibenzyl Ethers Targeting the Programmed Cell Death-1/Programmed Cell Death-Ligand 1 Interaction as Potential Anticancer Agents. J Med Chem. 2020 Aug 13;63(15):8338-8358. doi: 10.1021/acs.jmedchem.0c00574. Epub 2020 Jul 30. PMID: 32667799.

In vitro protocol:

In vivo protocol:

1: Cheng B, Ren Y, Niu X, Wang W, Wang S, Tu Y, Liu S, Wang J, Yang D, Liao G, Chen J. Discovery of Novel Resorcinol Dibenzyl Ethers Targeting the Programmed Cell Death-1/Programmed Cell Death-Ligand 1 Interaction as Potential Anticancer Agents. J Med Chem. 2020 Aug 13;63(15):8338-8358. doi: 10.1021/acs.jmedchem.0c00574. Epub 2020 Jul 30. PMID: 32667799.