6RK73 is a potent, selective, covalent, and irreversible inhibitor of ubiquitin C-terminal hydrolase L1 (UCHL1), an enzyme implicated in neurodegenerative diseases and cancer. In biochemical assays, 6RK73 inhibits recombinant UCHL1 with an IC₅₀ of 26 nM by covalently modifying the catalytic cysteine residue. It shows high selectivity over other deubiquitinases (DUBs), including UCHL3 and UCHL5, with >100-fold selectivity reported. In cell-based assays, 6RK73 effectively blocks UCHL1 activity at low micromolar concentrations, leading to accumulation of ubiquitinated proteins and perturbation of cellular ubiquitin homeostasis. Its irreversible covalent mechanism ensures sustained target engagement, making 6RK73 a valuable chemical probe for studying UCHL1’s role in protein degradation and neurobiology.
MedKoo Cat#: 462342
Name: 6RK73
CAS#: 1895050-66-4
Chemical Formula: C13H17N5O2S
Exact Mass: 307.1103
Molecular Weight: 307.37
Elemental Analysis: C, 50.80; H, 5.58; N, 22.79; O, 10.41; S, 10.43
The following data is based on the product molecular weight 307.37 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
