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MedKoo Cat#: 462220 | Name: Ro 32-0432 HCl
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Ro 32-0432 HCl is a potent, selective, ATP-competitive and orally active PKC inhibitor.

Chemical Structure

Ro 32-0432 HCl
Ro 32-0432 HCl
CAS#1781828-85-0

Theoretical Analysis

MedKoo Cat#: 462220

Name: Ro 32-0432 HCl

CAS#: 1781828-85-0

Chemical Formula: C28H29ClN4O2

Exact Mass:

Molecular Weight: 489.02

Elemental Analysis: C, 68.77; H, 5.98; Cl, 7.25; N, 11.46; O, 6.54

Price and Availability

Size Price Availability Quantity
1mg USD 395.00 2 Weeks
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Related CAS #
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Synonym
Ro 32-0432 hydrochloride, Ro 32-0432 HCl, Ro 32 0432 HCl, Ro 320432 HCl, Ro32-0432 HCl
IUPAC/Chemical Name
(S)-3-(8-((dimethylamino)methyl)-6,7,8,9-tetrahydropyrido[1,2-a]indol-10-yl)-4-(1-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione hydrochloride
InChi Key
HSPRASOZRZDELU-LMOVPXPDSA-N
InChi Code
InChI=1S/C28H28N4O2.ClH/c1-30(2)15-17-12-13-32-22-11-7-5-9-19(22)24(23(32)14-17)26-25(27(33)29-28(26)34)20-16-31(3)21-10-6-4-8-18(20)21;/h4-11,16-17H,12-15H2,1-3H3,(H,29,33,34);1H/t17-;/m0./s1
SMILES Code
O=C1NC(C(C2=C(C[C@@H](CN(C)C)CC3)N3C4=C2C=CC=C4)=C1C5=CN(C)C6=C5C=CC=C6)=O.[H]Cl
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Ro 32-0432 HCl is a potent, selective, ATP-competitive and orally active PKC inhibitor. The IC50 values of Ro 32-0432 HCl for PKCα, PKCβI, PKCβII, PKCγ and PKCε are 9.3 nM, 28 nM, 30 nM, 36.5 nM and 108.3 nM, respectively. Ro 32-0432 HCl prevents T-cell activation.
In vitro activity:
NECA-mediated IL-6 release was inhibited by 1-[6-((17beta-3-methoxyestra-1,3,5(10)-tiene-17-yl)amino)hexyl]-1H-pyrrole-2,5-dione, wortmannin, and Ro-32-0432. This study investigated the action of adenosine A2 receptor agonists on IL-6 and VEGF secretion in two murine FS cell lines (TtT/GF and Tpit/F1), and demonstrated a rank order of potency. Reference: Br J Pharmacol. 2003 Oct;140(4):764-72. https://pubmed.ncbi.nlm.nih.gov/14504137/
In vivo activity:
In vivo injection with chelerythrine chloride or Ro-32-0432 into the ventral tegmental area (VTA) significantly suppressed the place preference and increased levels of dopamine in the nucleus accumbens induced by intra-VTA injection of orexins. Reference: Eur J Neurosci. 2007 Mar;25(5):1537-45. https://pubmed.ncbi.nlm.nih.gov/17425580/
Solvent mg/mL mM
Solubility
DMSO 4.9 10.00
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 489.02 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Rees DA, Lewis BM, Lewis MD, Francis K, Scanlon MF, Ham J. Adenosine-induced IL-6 expression in pituitary folliculostellate cells is mediated via A2b adenosine receptors coupled to PKC and p38 MAPK. Br J Pharmacol. 2003 Oct;140(4):764-72. doi: 10.1038/sj.bjp.0705488. Epub 2003 Sep 22. PMID: 14504137; PMCID: PMC1574075. 2. Narita M, Nagumo Y, Miyatake M, Ikegami D, Kurahashi K, Suzuki T. Implication of protein kinase C in the orexin-induced elevation of extracellular dopamine levels and its rewarding effect. Eur J Neurosci. 2007 Mar;25(5):1537-45. doi: 10.1111/j.1460-9568.2007.05403.x. PMID: 17425580.
In vitro protocol:
1. Rees DA, Lewis BM, Lewis MD, Francis K, Scanlon MF, Ham J. Adenosine-induced IL-6 expression in pituitary folliculostellate cells is mediated via A2b adenosine receptors coupled to PKC and p38 MAPK. Br J Pharmacol. 2003 Oct;140(4):764-72. doi: 10.1038/sj.bjp.0705488. Epub 2003 Sep 22. PMID: 14504137; PMCID: PMC1574075.
In vivo protocol:
1. Narita M, Nagumo Y, Miyatake M, Ikegami D, Kurahashi K, Suzuki T. Implication of protein kinase C in the orexin-induced elevation of extracellular dopamine levels and its rewarding effect. Eur J Neurosci. 2007 Mar;25(5):1537-45. doi: 10.1111/j.1460-9568.2007.05403.x. PMID: 17425580.
1: Birchall AM, Bishop J, Bradshaw D, et al. Ro 32-0432, a selective and orally active inhibitor of protein kinase C prevents T-cell activation. J Pharmacol Exp Ther. 1994;268(2):922-929. 2: Singh TG, Rehni AK, Arora S. Ro 32-0432 attenuates mecamylamine-precipitated nicotine withdrawal syndrome in mice. Naunyn Schmiedebergs Arch Pharmacol. 2013;386(3):197-204. doi:10.1007/s00210-012-0825-0