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MedKoo Cat#: 462196 | Name: Nucleic Acid Dye Green I

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Nucleic Acid Dye Green I is a high affinity double-stranded DNA (dsDNA) probe that is used in RT-LAMP and RT-PCR.

Chemical Structure

Nucleic Acid Dye Green I
Nucleic Acid Dye Green I
CAS#2225748-05-8

Theoretical Analysis

MedKoo Cat#: 462196

Name: Nucleic Acid Dye Green I

CAS#: 2225748-05-8

Chemical Formula: C33H38ClN3S

Exact Mass: 543.2475

Molecular Weight: 544.20

Elemental Analysis: C, 72.83; H, 7.04; Cl, 6.51; N, 7.72; S, 5.89

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.
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Synonym
Nucleic Acid Dye Green I
IUPAC/Chemical Name
2-((2-((3-(Dimethylamino)propyl)(propyl)amino)-1-phenylquinolin-4(1H)-ylidene)methyl)-3-methylbenzo[d]thiazol-3-ium chloride
InChi Key
NNBNPKJXKFINFH-UHFFFAOYSA-M
InChi Code
InChI=1S/C33H38N3S.ClH/c1-5-14-25(15-13-22-34(2)3)31-23-26(24-33-35(4)30-20-11-12-21-32(30)37-33)28-18-9-10-19-29(28)36(31)27-16-7-6-8-17-27;/h6-12,16-21,23-25H,5,13-15,22H2,1-4H3;1H/q+1;/p-1
SMILES Code
CN(C)CCCC(CCC)C(N1C2=CC=CC=C2)=C/C(C3=C1C=CC=C3)=C/C4=[N+](C)C5=CC=CC=C5S4.[Cl-]
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 544.20 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Karlsen F, Steen HB, Nesland JM. SYBR green I DNA staining increases the detection sensitivity of viruses by polymerase chain reaction. J Virol Methods. 1995;55(1):153-156. doi:10.1016/0166-0934(95)00053-w 2: Bookout AL, Cummins CL, Mangelsdorf DJ, Pesola JM, Kramer MF. High-throughput real-time quantitative reverse transcription PCR. Curr Protoc Mol Biol. 2006;Chapter 15:. doi:10.1002/0471142727.mb1508s73. 3:Lamb LE, Bartolone SN, Tree MO, et al. Rapid Detection of Zika Virus in Urine Samples and Infected Mosquitos by Reverse Transcription-Loop-Mediated Isothermal Amplification. Sci Rep. 2018;8(1):3803. Published 2018 Feb 28. doi:10.1038/s41598-018-22102-5.

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