RS-09 | CAS#1449566-36-2 | TLR4 agonist

MedKoo Cat#: 574392 | Name: RS-09

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

RS-09 is a TLR4 agonist that promotes NF-κB nuclear translocation and induces inflammatory cytokine secretion in RAW264.7 macrophages in vitro. It acts as an adjuvant in vivo and enhances X-15 specific antibody serum concentrations.

Chemical Structure

RS-09

CAS#1449566-36-2

Theoretical Analysis

MedKoo Cat#: 574392

Name: RS-09

CAS#: 1449566-36-2

Chemical Formula: C31H49N9O9

Exact Mass: 691.3653

Molecular Weight: 691.79

Elemental Analysis: C, 53.82; H, 7.14; N, 18.22; O, 20.81

Price and Availability

Size	Price	Availability	Quantity
1mg	USD 450.00	2 Weeks

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Synonym

RS-09, RS09, RS 09

IUPAC/Chemical Name

L-alanyl-L-prolyl-L-prolyl-L-histidyl-L-alanyl-L-leucyl-L-serine

InChi Key

SHYNPSTXGFDOBD-HRALPVODSA-N

InChi Code

InChI=1S/C31H49N9O9/c1-16(2)11-20(27(44)38-22(14-41)31(48)49)36-25(42)18(4)35-26(43)21(12-19-13-33-15-34-19)37-28(45)23-7-5-9-39(23)30(47)24-8-6-10-40(24)29(46)17(3)32/h13,15-18,20-24,41H,5-12,14,32H2,1-4H3,(H,33,34)(H,35,43)(H,36,42)(H,37,45)(H,38,44)(H,48,49)/t17-,18-,20-,21-,22-,23-,24-/m0/s1

SMILES Code

[H]N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

RS09 increases antibody production in a vaccine setting.

In vitro activity:

This study reports the identification and activity of several peptides which functionally mimicked LPS (one beinh RS-09). The activity of the LPS-TLR-4 interaction was assessed by NF-κB nuclear translocation analyses in HEK-BLUE™-4 cells, a cell culture model that expresses only TLR-4, and the murine macrophage cell line, RAW264.7. Reference: PLoS One. 2012;7(2):e30839. https://pubmed.ncbi.nlm.nih.gov/22363498/

In vivo activity:

Both chemically linked (DP-RS09) and physically mixed RS09 (DP/RS09) could further enhance the mucosal immunity of rAd5 vaccine via TLR-4 pathway. This RS09 adjuvanted DEG-PEI polymer represents a potential intranasal vaccine delivery system and may have a wider application for other viral vectors. Reference: Mol Pharm. 2016 Mar 7;13(3):885-94. https://pubmed.ncbi.nlm.nih.gov/26824411/

Preparing Stock Solutions

The following data is based on the product molecular weight 691.79 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Shanmugam A, Rajoria S, George AL, Mittelman A, Suriano R, Tiwari RK. Synthetic Toll like receptor-4 (TLR-4) agonist peptides as a novel class of adjuvants. PLoS One. 2012;7(2):e30839. doi: 10.1371/journal.pone.0030839. Epub 2012 Feb 20. PMID: 22363498; PMCID: PMC3282694. 2. Li M, Jiang Y, Gong T, Zhang Z, Sun X. Intranasal Vaccination against HIV-1 with Adenoviral Vector-Based Nanocomplex Using Synthetic TLR-4 Agonist Peptide as Adjuvant. Mol Pharm. 2016 Mar 7;13(3):885-94. doi: 10.1021/acs.molpharmaceut.5b00802. Epub 2016 Feb 15. PMID: 26824411.

In vitro protocol:

In vivo protocol:

1. Li M, Jiang Y, Gong T, Zhang Z, Sun X. Intranasal Vaccination against HIV-1 with Adenoviral Vector-Based Nanocomplex Using Synthetic TLR-4 Agonist Peptide as Adjuvant. Mol Pharm. 2016 Mar 7;13(3):885-94. doi: 10.1021/acs.molpharmaceut.5b00802. Epub 2016 Feb 15. PMID: 26824411.

1: Dhanushkumar T, Selvam PK, M E S, Vasudevan K, C GPD, Zayed H, Kamaraj B. Rational design of a multivalent vaccine targeting arthropod-borne viruses using reverse vaccinology strategies. Int J Biol Macromol. 2024 Feb;258(Pt 1):128753. doi: 10.1016/j.ijbiomac.2023.128753. Epub 2023 Dec 15. PMID: 38104690. 2: Basmenj ER, Arastonejad M, Mamizadeh M, Alem M, KhalatbariLimaki M, Ghiabi S, Khamesipour A, Majidiani H, Shams M, Irannejad H. Engineering and design of promising T-cell-based multi-epitope vaccine candidates against leishmaniasis. Sci Rep. 2023 Nov 8;13(1):19421. doi: 10.1038/s41598-023-46408-1. PMID: 37940672; PMCID: PMC10632461. 3: Chen Y, Song Z, Chang H, Guo Y, Wei Z, Sun Y, Gong L, Zheng Z, Zhang G. Dihydromyricetin inhibits African swine fever virus replication by downregulating toll-like receptor 4-dependent pyroptosis in vitro. Vet Res. 2023 Jul 12;54(1):58. doi: 10.1186/s13567-023-01184-8. PMID: 37438783; PMCID: PMC10337113. 4: Shams M, Maleki B, Kordi B, Majidiani H, Nazari N, Irannejad H, Asghari A. Towards the First Multiepitope Vaccine Candidate against Neospora caninum in Mouse Model: Immunoinformatic Standpoint. Biomed Res Int. 2022 Jun 9;2022:2644667. doi: 10.1155/2022/2644667. PMID: 35722460; PMCID: PMC9204498. 5: Frontiers Production Office. Erratum: Lactobacillus plantarum RS-09 Induces M1-Type Macrophage Immunity Against Salmonella typhimurium Challenge via the TLR2/NF-κB Signalling Pathway. Front Pharmacol. 2022 Jun 3;13:937618. doi: 10.3389/fphar.2022.937618. Erratum for: Front Pharmacol. 2022 Mar 07;13:832245. doi: 10.3389/fphar.2022.832245. PMID: 35721138; PMCID: PMC9204476. 6: Zhao X, Zhao C, Yang L, Jiang L, Zhang J, Yu X, Chen G, Zhu H, Tang W, Li Y, Wei M, Zhang X, Jia H. Spatial and Temporal Persistence of Fluorescent Lactiplantibacillus plantarum RS-09 in Intestinal Tract. Front Microbiol. 2022 Mar 30;13:843650. doi: 10.3389/fmicb.2022.843650. PMID: 35432246; PMCID: PMC9006167. 7: Zhao C, Chen H, Liang H, Zhao X, Tang W, Wei M, Li Y, Zhang J, Yu X, Chen G, Zhu H, Jiang L, Zhang X. Lactobacillus plantarum RS-09 Induces M1-Type Macrophage Immunity Against Salmonella Typhimurium Challenge via the TLR2/NF-κB Signalling Pathway. Front Pharmacol. 2022 Mar 7;13:832245. doi: 10.3389/fphar.2022.832245. Erratum in: Front Pharmacol. 2022 Jun 03;13:937618. doi: 10.3389/fphar.2022.937618. PMID: 35355723; PMCID: PMC8959098. 8: Li D, Guo YY, Cen XF, Qiu HL, Chen S, Zeng XF, Zeng Q, Xu M, Tang QZ. Lupeol protects against cardiac hypertrophy via TLR4-PI3K-Akt-NF-κB pathways. Acta Pharmacol Sin. 2022 Aug;43(8):1989-2002. doi: 10.1038/s41401-021-00820-3. Epub 2021 Dec 16. PMID: 34916609; PMCID: PMC9343642. 9: Shams M, Nourmohammadi H, Majidiani H, Shariatzadeh SA, Asghari A, Fatollahzadeh M, Irannejad H. Engineering a multi-epitope vaccine candidate against Leishmania infantum using comprehensive Immunoinformatics methods. Biologia (Bratisl). 2022;77(1):277-289. doi: 10.1007/s11756-021-00934-3. Epub 2021 Nov 29. PMID: 34866641; PMCID: PMC8628819. 10: Shawcross P, Lyons M, Filingeri V. The relationship between readiness to change pain-related exercise participation and perceived work ability: a cross- sectional study of factory workers. BMC Musculoskelet Disord. 2021 Sep 6;22(1):762. doi: 10.1186/s12891-021-04642-6. PMID: 34488707; PMCID: PMC8419917. 11: Rehman A, Ahmad S, Shahid F, Albutti A, Alwashmi ASS, Aljasir MA, Alhumeed N, Qasim M, Ashfaq UA, Tahir Ul Qamar M. Integrated Core Proteomics, Subtractive Proteomics, and Immunoinformatics Investigation to Unveil a Potential Multi- Epitope Vaccine against Schistosomiasis. Vaccines (Basel). 2021 Jun 16;9(6):658. doi: 10.3390/vaccines9060658. PMID: 34208663; PMCID: PMC8235758. 12: Asghari A, Shamsinia S, Nourmohammadi H, Majidiani H, Fatollahzadeh M, Nemati T, Irannejad H, Nouri HR, Ghasemi E, Shams M. Development of a chimeric vaccine candidate based on Toxoplasma gondii major surface antigen 1 and apicoplast proteins using comprehensive immunoinformatics approaches. Eur J Pharm Sci. 2021 Jul 1;162:105837. doi: 10.1016/j.ejps.2021.105837. Epub 2021 Apr 6. PMID: 33836177. 13: Vani MF, Sabiston CM, Petrella A, Adams SC, Eaton G, Chalifour K, Garland SN. Body image concerns of young adult cancer survivors: A brief report. J Psychosoc Oncol. 2021;39(5):673-679. doi: 10.1080/07347332.2020.1815926. Epub 2020 Sep 9. PMID: 32902366. 14: du Pont A, Rhee SH, Corley RP, Hewitt JK, Friedman NP. Rumination and executive functions: Understanding cognitive vulnerability for psychopathology. J Affect Disord. 2019 Sep 1;256:550-559. doi: 10.1016/j.jad.2019.06.026. Epub 2019 Jun 22. PMID: 31280080; PMCID: PMC6751021. 15: Wilson KM, Millner AJ, Auerbach RP, Glenn CR, Kearns JC, Kirtley OJ, Najmi S, O'Connor RC, Stewart JG, Cha CB. Investigating the psychometric properties of the Suicide Stroop Task. Psychol Assess. 2019 Aug;31(8):1052-1061. doi: 10.1037/pas0000723. Epub 2019 May 9. PMID: 31070448; PMCID: PMC7011179. 16: Bergsma-Vlami M, van de Bilt JLJ, Tjou-Tam-Sin NNA, Westenberg M, Meekes ETM, Teunissen HAS, Van Vaerenbergh J. Phylogenetic Assignment of Ralstonia pseudosolanacearum (Ralstonia solanacearum Phylotype I) Isolated from Rosa spp. Plant Dis. 2018 Nov;102(11):2258-2267. doi: 10.1094/PDIS-09-17-1345-RE. Epub 2018 Sep 4. PMID: 30192708. 17: Asolkar T, Ramesh R. Development of T3SS Mutants (hrpB- and hrcV-) of Ralstonia solanacearum, Evaluation of Virulence Attenuation in Brinjal and Tomato-A Pre-requisite to Validate T3Es of R. solanacearum. Indian J Microbiol. 2018 Sep;58(3):372-380. doi: 10.1007/s12088-018-0736-y. Epub 2018 May 2. PMID: 30013282; PMCID: PMC6023813. 18: Asolkar T, Ramesh R. Identification of virulence factors and type III effectors of phylotype I, Indian Ralstonia solanacearum strains Rs-09-161 and Rs-10-244. J Genet. 2018 Mar;97(1):55-66. PMID: 29666325. 19: Le BM, Impett EA, Lemay EP, Muise A, Tskhay KO. Communal motivation and well-being in interpersonal relationships: An integrative review and meta- analysis. Psychol Bull. 2018 Jan;144(1):1-25. doi: 10.1037/bul0000133. Epub 2017 Nov 20. PMID: 29154556. 20: Ahmad AA, Stulberg MJ, Mershon JP, Mollov DS, Huang Q. Molecular and biological characterization of ϕRs551, a filamentous bacteriophage isolated from a race 3 biovar 2 strain of Ralstonia solanacearum. PLoS One. 2017 Sep 21;12(9):e0185034. doi: 10.1371/journal.pone.0185034. PMID: 28934297; PMCID: PMC5608472.