1: Abuin E, Lissi E, Ahumada M, Calderón C. Effect of human serum albumin on the kinetics of N-glutaryl-L-phenylalanine p-nitroanilide hydrolysis catalyzed by α-chymotrypsin. Protein J. 2011 Feb;30(2):143-7. doi: 10.1007/s10930-011-9315-3. PMID: 21336975.
2: Malthouse JPG. Kinetic Studies of the Effect of pH on the Trypsin-Catalyzed Hydrolysis of N-α-benzyloxycarbonyl-l-lysine-p-nitroanilide: Mechanism of Trypsin Catalysis. ACS Omega. 2020 Mar 3;5(10):4915-4923. doi: 10.1021/acsomega.9b03750. PMID: 32201777; PMCID: PMC7081292.
3: Abuin E, Lissi E, Calderón C. Kinetics of N-glutaryl-L-phenylalanine p-nitroanilide hydrolysis catalyzed by alpha-chymotrypsin in aqueous solutions of alkyltrimethylammonium bromides. J Colloid Interface Sci. 2007 Apr 15;308(2):573-6. doi: 10.1016/j.jcis.2007.01.007. Epub 2007 Jan 10. PMID: 17250849.
4: Theodorsen L, Stromme JH. Gamma-Glutamyl-3-carboxy-14-nitroanilide: the substrate of choice for routine determinations of gamma-glutamyl-transferase activity in serum? Clin Chim Acta. 1976 Oct 15;72(2):205-10. doi: 10.1016/0009-8981(76)90074-7. PMID: 10105.
5: Shaw LM, London JW, Fetterolf D, Garfinkel D. Gamma-Glutamyltransferase: kinetic properties and assay conditions when gamma-glutamyl-4-nitroanilide and its 3-carboxy derivative are used as donor substrates. Clin Chem. 1977 Jan;23(1):79-85. PMID: 12882.
6: Bozić N, Vujcić Z. Detection and quantification of leucyl aminopeptidase after native electrophoresis using leucine-p-nitroanilide. Electrophoresis. 2005 Jun;26(12):2476-80. doi: 10.1002/elps.200500047. PMID: 15920779.
7: Abe K, Watanabe N, Kosaka T, Yamada M, Tokui T, Ikeda T. Hydrolysis of synthetic substrate, L-pyroglutamyl p-nitroanilide is catalyzed solely by pyroglutamyl aminopeptidase I in rat liver cytosol. Biol Pharm Bull. 2003 Nov;26(11):1528-33. doi: 10.1248/bpb.26.1528. PMID: 14600395.
8: Sharma SK, Castellino FJ. The chemical synthesis of the chromogenic substrates, H-D-Val-L-Leu-L-Lys-p-nitroanilide (S2251) and H-D-Ile-L-Pro-L-ARG- p-nitroanilide (S2288). Thromb Res. 1990 Jan 1;57(1):127-38. doi: 10.1016/0049-3848(90)90201-m. PMID: 2300919.
9: Wei D, Huang X, Liu J, Tang M, Zhan CG. Reaction pathway and free energy profile for papain-catalyzed hydrolysis of N-acetyl-Phe-Gly 4-nitroanilide. Biochemistry. 2013 Jul 30;52(30):5145-54. doi: 10.1021/bi400629r. Epub 2013 Jul 17. PMID: 23862626; PMCID: PMC3770148.
10: Tokai S, Bito T, Shimizu K, Arima J. Methionine residues lining the substrate pathway in prolyl oligopeptidase from Pleurotus eryngii play an important role in substrate recognition. Biosci Biotechnol Biochem. 2018 Jul;82(7):1107-1115. doi: 10.1080/09168451.2018.1459177. Epub 2018 Apr 6. PMID: 29623768.
11: Fanuel L, Thamm I, Kostanjevecki V, Samyn B, Joris B, Goffin C, Brannigan J, Van Beeumen J, Frère JM. Two new aminopeptidases from Ochrobactrum anthropi active on D-alanyl-p-nitroanilide. Cell Mol Life Sci. 1999 May;55(5):812-8. doi: 10.1007/s000180050334. PMID: 10379365.
12: Tanaka T, McRae BJ, Cho K, Cook R, Fraki JE, Johnson DA, Powers JC. Mammalian tissue trypsin-like enzymes. Comparative reactivities of human skin tryptase, human lung tryptase, and bovine trypsin with peptide 4-nitroanilide and thioester substrates. J Biol Chem. 1983 Nov 25;258(22):13552-7. PMID: 6358206.
13: Pelletier A, Dimicoli JL, Boudier C, Bieth JG. Nonchromogenic hydrolysis of elastase and cathepsin G p-nitroanilide substrates by Pseudomonas aeruginosa elastase. Am J Respir Cell Mol Biol. 1989 Jul;1(1):37-9. doi: 10.1165/ajrcmb/1.1.37. PMID: 2516450.
14: Shechter Y, Gertler A. Enhancement of alpha-chymotrypsin-catalyzed hydrolysis of specific p-nitroanilide substrates by 4-phenylbutylamine derivative of hen egg-white lysozyme. Biochim Biophys Acta. 1978 Nov 10;527(1):42-55. doi: 10.1016/0005-2744(78)90254-1. PMID: 718965.
15: Lottenberg R, Hall JA, Blinder M, Binder EP, Jackson CM. The action of thrombin on peptide p-nitroanilide substrates. Substrate selectivity and examination of hydrolysis under different reaction conditions. Biochim Biophys Acta. 1983 Feb 15;742(3):539-57. doi: 10.1016/0167-4838(83)90272-8. PMID: 6838888.
16: Hojo K, Maeda M, Iguchi S, Smith T, Okamoto H, Kawasaki K. Amino acids and peptides. XXXV. Facile preparation of p-nitroanilide analogs by the solid-phase method. Chem Pharm Bull (Tokyo). 2000 Nov;48(11):1740-4. doi: 10.1248/cpb.48.1740. PMID: 11086904.
17: Tanaka H, Shimazu T, Sugimoto H, Yoshioka T, Sugimoto T. A sensitive and specific assay for granulocyte elastase in inflammatory tissue fluid using L-pyroglutamyl-L-prolyl-L-valine-p-nitroanilide. Clin Chim Acta. 1990 Feb 28;187(2):173-80. doi: 10.1016/0009-8981(90)90344-r. PMID: 2317934.
18: Magalhães HP, Magalhães A, Juliano L, Nelson DL, Rogana E. Mechanism of action and determination of the best substrate for a thrombin-like enzyme from Lachesis muta muta venom by regression analysis of the kinetic parameters determined with peptidyl p-nitroanilide substrates. Toxicon. 2006 Mar 15;47(4):453-8. doi: 10.1016/j.toxicon.2006.01.001. Epub 2006 Feb 20. PMID: 16488459.
19: Sousa MO, Miranda TL, Maia CN, Bittar ER, Santoro MM, Figueiredo AF. Kinetic peculiarities of human tissue kallikrein: 1--substrate activation in the catalyzed hydrolysis of H-D-valyl-L-leucyl-L-arginine 4-nitroanilide and H-D- valyl-L-leucyl-L-lysine 4-nitroanilide; 2--substrate inhibition in the catalyzed hydrolysis of N alpha-p-tosyl-L-arginine methyl ester. Arch Biochem Biophys. 2002 Apr 1;400(1):7-14. doi: 10.1006/abbi.2002.2764. PMID: 11913965.
20: Ohlsson BG, Weström BR, Karlsson BW. Enzymoblotting: a method for localizing proteinases and their zymogens using para-nitroanilide substrates after agarose gel electrophoresis and transfer to nitrocellulose. Anal Biochem. 1986 Feb 1;152(2):239-44. doi: 10.1016/0003-2697(86)90404-5. PMID: 3516006.