CADD522 is a potent inhibitor of runt-related transcription factor-2 (RUNX2)-DNA binding with an IC50 of 10 nM. CADD522 treatment resulted in significant growth inhibition, clonogenic survival, tumorsphere formation, and invasion of breast cancer cells. CADD522 negatively regulated transcription of RUNX2 target genes such as matrix metalloproteinase-13, vascular endothelial growth factor and glucose transporter-1, but upregulated RUNX2 expression by increasing RUNX2 stability. CADD522 reduced RUNX2-mediated increases in glucose uptake and decreased the level of CBF-β and RUNX2 phosphorylation at the S451 residue.
MedKoo Cat#: 408095
Name: CADD522
CAS#: 199735-88-1
Chemical Formula: C15H13Cl2NO3
Exact Mass: 325.0272
Molecular Weight: 326.17
Elemental Analysis: C, 55.24; H, 4.02; Cl, 21.74; N, 4.29; O, 14.72
| Solvent | mg/mL | mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 157.5 | 482.87 | |
| Ethanol | 65.0 | 199.28 |
The following data is based on the product molecular weight 326.17 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
