MedKoo Cat#: 558986 | Name: Cyanine3.5 NHS ester
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Cyanine3.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. Cyanine3.5 NHS ester can replace NHS esters of Cy3.5, Alexa Fluor 594, DyLight 594.

Chemical Structure

Cyanine3.5 NHS ester
Cyanine3.5 NHS ester
CAS#2231670-85-0

Theoretical Analysis

MedKoo Cat#: 558986

Name: Cyanine3.5 NHS ester

CAS#: 2231670-85-0

Chemical Formula: C42H44BF4N3O4

Exact Mass: 0.0000

Molecular Weight: 741.63

Elemental Analysis: C, 68.02; H, 5.98; B, 1.46; F, 10.25; N, 5.67; O, 8.63

Price and Availability

Size Price Availability Quantity
5mg USD 550.00 2 Weeks
25mg USD 1,250.00 2 Weeks
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Synonym
Cyanine3.5 NHS ester; Cyanine 3.5 NHS ester; Cyanine-3.5-NHS ester
IUPAC/Chemical Name
3-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-1,1-dimethyl-2-((1E,3E)-3-(1,1,3-trimethyl-1,3-dihydro-2H-benzo[e]indol-2-ylidene)prop-1-en-1-yl)-1H-benzo[e]indol-3-ium tetrafluoroborate
InChi Key
VVRSBJZTKKPGEM-UHFFFAOYSA-N
InChi Code
InChI=1S/C42H44N3O4.BF4/c1-41(2)34(43(5)32-23-21-28-14-8-10-16-30(28)39(32)41)18-13-19-35-42(3,4)40-31-17-11-9-15-29(31)22-24-33(40)44(35)27-12-6-7-20-38(48)49-45-36(46)25-26-37(45)47;2-1(3,4)5/h8-11,13-19,21-24H,6-7,12,20,25-27H2,1-5H3;/q+1;-1
SMILES Code
CN1/C(C(C)(C)C2=C1C=CC3=CC=CC=C23)=C/C=C/C4=[N+](CCCCCC(ON5C(CCC5=O)=O)=O)C6=C(C7=CC=CC=C7C=C6)C4(C)C.F[B-](F)(F)F
Appearance
Solid powder
Purity
>95% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 741.63 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1. Lee, S.A.; Biteen, J.S. Spectral Reshaping of Single Dye Molecules Coupled to Single Plasmonic Nanoparticles. Journal of Physical Chemistry Letters, 2019, 10, 5764–5769. doi: 10.1021/acs.jpclett.9b02480 2. Kravchuk, O.I.; Lyupina, Y.V.; Erokhov, P.A.; Finoshin, A.D.; Adameyko, K.I.; Mishyna, M.Y.; Moiseenko, A.V.; Sokolova, O.S.; Orlova, O.V.; Beljelarskaya, S.N.; Serebryakova, M.V.; Indeykina, M.I.; Bugrova, A.E.; Kononikhin, A.S.; Mikhailov, V.S. Characterization of the 20S proteasome of the lepidopteran, Spodoptera frugiperda. Biochimica et Biophysica Acta - Proteins and Proteomics, 2019, 1867(9), 840–853. doi: 10.1016/j.bbapap.2019.06.010 3. Wang, C.; Niederstrasser, H.; Douglas, P.M.; Lin, R.; Jaramillo, J.; Li, Y.; Olswald, N.W.; Zhou, A.; McMillan, E.A.; Mendiratta, S.; Wang, Z.; Zhao, T.; Lin, Z.; Luo, M.; Huang, G.; Brekken, R.A.; Posner, B.A.; MacMillan, J.B.; Gao, J.; White, M.A. Small-molecule TFEB pathway agonists that ameliorate metabolic syndrome in mice and extend C. elegans lifespan. Nature Communications, 2017, 8, 2270. doi: 10.1038/s41467-017-02332-3 4. Massey, M.; Kim, H.; Conroy, E.M.; Algar, W.R. Expanded Quantum Dot-Based Concentric Förster Resonance Energy Transfer: Adding and Characterizing Energy-Transfer Pathways for Triply Multiplexed Biosensing. The Journal of Physical Chemistry C, 2017, 121(24), 13345–13356. doi: 10.1021/acs.jpcc.7b02739 5.Wang, C.; Wang, Y.; Li, Y.; Bodemann, B.; Zhao, T.; Ma, X.; Huang, G.; Hu, Z.; DeBerardinis, R.J.; White, M.A.; Gao, J. A nanobuffer reporter library for fine-scale imaging and perturbation of endocytic organelles. Nature Communications, 2015, 6, 8524. doi: 10.1038/ncomms9524 6. Fu, B.; Flynn, J.D.; Isaacoff, B.P.; Rowland, D.J.; Biteen, J.S. Super-Resolving the Distance-Dependent Plasmon-Enhanced Fluorescence of Single Dye and Fluorescent Protein Molecules. The Journal of Physical Chemistry C, 2015, 119(33), 19350–19358. doi: 10.1021/acs.jpcc.5b05154