JOE is a xanthene dye, fluorescein derivative possessing two chloro and two methoxy substituents. This fluorophore is a useful label for oligonucleotides. Its absorption and emission spectra are located between FAM and TAMRA. The fluorophore can be introduced into oligonucleotide using this phosphoramidite. It tolerates standard ammonium deblock conditions. This product contains pure isomer of 5-JOE dye.
MedKoo Cat#: 558948
Name: JOE phosphoramidite, 5-isomer
CAS#: N/A
Chemical Formula: C48H60Cl2N3O12P
Exact Mass: 971.3292
Molecular Weight: 972.89
Elemental Analysis: C, 59.26; H, 6.22; Cl, 7.29; N, 4.32; O, 19.73; P, 3.18
The following data is based on the product molecular weight 972.89 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
