NNMTi | CAS#42464-96-0 | inhibitor

MedKoo Cat#: 585400 | Name: NNMTi

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

NNMTi is a nicotinamide N-methyltransferase (NNMT) inhibitor that promotes myoblast differentiation.

Chemical Structure

NNMTi

CAS#42464-96-0

Theoretical Analysis

MedKoo Cat#: 585400

Name: NNMTi

CAS#: 42464-96-0

Chemical Formula: C10H11IN2

Exact Mass: 285.9967

Molecular Weight: 286.12

Elemental Analysis: C, 41.98; H, 3.88; I, 44.35; N, 9.79

Price and Availability

Size	Price	Availability	Quantity
10mg	USD 280.00
50mg	USD 710.00

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Synonym

NNMTi

IUPAC/Chemical Name

5-Amino-1-methylquinolinium iodide

InChi Key

JPEZFBFIRRAFNR-UHFFFAOYSA-N

InChi Code

InChI=1S/C10H10N2.HI/c1-12-7-3-4-8-9(11)5-2-6-10(8)12;/h2-7,11H,1H3;1H

SMILES Code

C[N+]1=C2C=CC=C(N)C2=CC=C1.[I-]

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Certificate of Analysis

View CoA: current batch, Lot# T20O09R23

Safety Data Sheet (SDS)

View Safety Data Sheet (SDS)

Instruction

View handling instruction

Biological target:

NNMTi is a potent nicotinamide N-methyltransferase (NNMT) inhibitor (IC50=1.2 μM).

In vitro activity:

Importantly, improved muSC activity translated not only to larger myofibers after injury but also to greater contractile function, with the peak torque of the TA increased by ∼70% in NNMTi-treated mice compared to controls. Similar results were recapitulated in vitro with C2C12 myoblasts, where NNMTi treatment promoted and enhanced myoblast differentiation with supporting changes in the cellular NAD+/NADH redox states. Reference: Biochem Pharmacol. 2019 May;163:481-492. https://pubmed.ncbi.nlm.nih.gov/30753815/

In vivo activity:

The present study investigated metabolic and physiological benefits of nicotinamide N-methyltransferase inhibitor (NNMTi) treatment combined with a lean diet substitution in diet-induced obese mice. Importantly, combined lean diet and NNMTi treatment normalized body composition and liver adiposity parameters to levels observed in age-matched lean diet control mice. NNMTi treatment produced a unique metabolomic signature in adipose tissue, with predominant increases in ketogenic amino acid abundance and alterations to metabolites linked to energy metabolic pathways. Reference: Sci Rep. 2021 Mar 11;11(1):5637. https://pubmed.ncbi.nlm.nih.gov/33707534/

	Solvent	mg/mL	mM
Solubility
	DMSO	56.3	196.82
	Ethanol	3.0	10.49
	Water	3.6	12.70

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 286.12 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Neelakantan H, Brightwell CR, Graber TG, Maroto R, Wang HL, McHardy SF, Papaconstantinou J, Fry CS, Watowich SJ. Small molecule nicotinamide N-methyltransferase inhibitor activates senescent muscle stem cells and improves regenerative capacity of aged skeletal muscle. Biochem Pharmacol. 2019 May;163:481-492. doi: 10.1016/j.bcp.2019.02.008. Epub 2019 Feb 10. PMID: 30753815; PMCID: PMC6469996. 2. Dimet-Wiley A, Wu Q, Wiley JT, Eswar A, Neelakantan H, Savidge T, Watowich S. Reduced calorie diet combined with NNMT inhibition establishes a distinct microbiome in DIO mice. Sci Rep. 2022 Jan 10;12(1):484. doi: 10.1038/s41598-021-03670-5. PMID: 35013352; PMCID: PMC8748953. 3. Sampson CM, Dimet AL, Neelakantan H, Ogunseye KO, Stevenson HL, Hommel JD, Watowich SJ. Combined nicotinamide N-methyltransferase inhibition and reduced-calorie diet normalizes body composition and enhances metabolic benefits in obese mice. Sci Rep. 2021 Mar 11;11(1):5637. doi: 10.1038/s41598-021-85051-6. PMID: 33707534; PMCID: PMC7952898.

In vitro protocol:

In vivo protocol:

1. Dimet-Wiley A, Wu Q, Wiley JT, Eswar A, Neelakantan H, Savidge T, Watowich S. Reduced calorie diet combined with NNMT inhibition establishes a distinct microbiome in DIO mice. Sci Rep. 2022 Jan 10;12(1):484. doi: 10.1038/s41598-021-03670-5. PMID: 35013352; PMCID: PMC8748953. 2. Sampson CM, Dimet AL, Neelakantan H, Ogunseye KO, Stevenson HL, Hommel JD, Watowich SJ. Combined nicotinamide N-methyltransferase inhibition and reduced-calorie diet normalizes body composition and enhances metabolic benefits in obese mice. Sci Rep. 2021 Mar 11;11(1):5637. doi: 10.1038/s41598-021-85051-6. PMID: 33707534; PMCID: PMC7952898.

Neelakantan et al (2019) Small molecule nicotinamide N-methyltransferase inhibitor activates senescent muscle stem cells and improves regenerative capacity of aged skeletal muscle. Biochem. Pharmacol. 163 481 PMID: 30753815 Neelakantan et al (2017) Structure-activity relationship for small molecule inhibitors of nicotinamide N-methyltransferase. J.Med.Chem. 60 5015 PMID: 28548833