ABTS is a radical cation and a peroxidase substrate. It is used to assess antioxidant capacity in the Trolox equivalent antioxidant capacity (TEAC) assay. It is blue in the presence of sodium persulfate or metmyoglobin but decolorizes upon incubation with antioxidants, and the antioxidant capacity can be determined spectrophotometrically. ABTS has also been used as an enzyme substrate in ELISAs. Upon incubation with a peroxidase, it produces a soluble green product that can be quantified by colorimetric detection at 405 nm.
MedKoo Cat#: 574167
Name: ABTS
CAS#: 30931-67-0
Chemical Formula: C18H24N6O6S4
Exact Mass: 548.0640
Molecular Weight: 548.67
Elemental Analysis: C, 39.40; H, 4.41; N, 15.32; O, 17.50; S, 23.37
The following data is based on the product molecular weight 548.67 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
