Homovanillic acid is a dopamine metabolite formed via deamination of dopamine by monoamine oxidase (MAO) to produce DOPAC followed by DOPAC metabolism by catechol-O-methyltransferase (COMT). HVA undergoes hydrogen peroxide-dependent oxidation in the presence of horseradish peroxidase to form a fluorescent dimer that displays excitation/emission maxima of 312/420 nm, respectively. It has been used to quantify hydrogen peroxide production in macrophages and neutrophils.
MedKoo Cat#: 574163
Name: Homovanillic acid
CAS#: 306-08-1
Chemical Formula: C9H10O4
Exact Mass: 182.0579
Molecular Weight: 182.17
Elemental Analysis: C, 59.34; H, 5.53; O, 35.13
The following data is based on the product molecular weight 182.17 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |