Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides). Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling. Labeling with Cyanine3 maleimide is selective, and efficient. We recommend using water-soluble Sulfo-Cyanine3 maleimide for the labeling of antibodies and other sensitive proteins.
MedKoo Cat#: 558829
Name: Cyanine3 maleimide
CAS#: 1593644-29-1 (without anion)
Chemical Formula: C36H43BF4N4O3
Exact Mass: 0.0000
Molecular Weight: 666.57
Elemental Analysis: C, 64.87; H, 6.50; B, 1.62; F, 11.40; N, 8.41; O, 7.20
The following data is based on the product molecular weight 666.57 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
