MedKoo Cat#: 574080 | Name: 6-TAMRA
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

6-TAMRA is a fluorescent dye that has commonly been used for the covalent labeling of oligonucleotides for DNA analysis. It displays excitation/emission maxima of 543/572 nm, respectively. 6-Carboxytetramethylrhodamine has been used in various DNA-protein binding studies, DNA FRET experiments, and as a standard reporter or quencher dye in RT-PCR.

Chemical Structure

6-TAMRA
6-TAMRA
CAS#91809-67-5

Theoretical Analysis

MedKoo Cat#: 574080

Name: 6-TAMRA

CAS#: 91809-67-5

Chemical Formula: C25H22N2O5

Exact Mass: 430.1529

Molecular Weight: 430.46

Elemental Analysis: C, 69.76; H, 5.15; N, 6.51; O, 18.58

Price and Availability

Size Price Availability Quantity
50mg USD 250.00 2 weeks
100mg USD 450.00 2 weeks
250mg USD 850.00 2 weeks
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Related CAS #
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Synonym
6-Carboxytetramethylrhodamine; 6-TAMRA; 6TAMRA; 6 TAMRA
IUPAC/Chemical Name
9-(2,5-dicarboxyphenyl)-3,6-bis(dimethylamino)-xanthylium, inner salt
InChi Key
COCMHKNAGZHBDZ-UHFFFAOYSA-N
InChi Code
InChI=1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)20-11-14(24(28)29)5-8-17(20)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
SMILES Code
[O-]C(C(C=CC(C(O)=O)=C1)=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)=O
Appearance
Very Dark Green to Black Solid
Purity
>90% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Acetonitrile (Slightly), DMSO (Slightly), Methanol (Very Slightly, Heated)
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Emission: 572 nm Excitation: 543 nm λmax: 253, 353, 546 nm

Preparing Stock Solutions

The following data is based on the product molecular weight 430.46 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1. Kvach, M.V., Stepanova, I.A., Prokhorenko, I.A., et al. Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes. Bioconjug. Chem. 20(8), 1673-1682 (2009). 2. Han, C., Chen, T., and Zu, L. Terbium chelate as donor label in time-resolved fluorescence energy transfer study of DNA hybridization. Chem. Phys. Lett. 500(4-6), 323-326 (2010).