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MedKoo Cat#: 574010 | Name: Suc-YVAD-pNA
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Suc-YVAD-pNA is a colorimetric substrate for caspase-1/interleukin-1β-converting enzyme (ICE) and caspase-4. Caspase-1 and caspase-4 preferentially bind to and cleave the Tyr-Val-Ala-Asp (YVAD) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of caspase-1 and caspase-4 activity.

Chemical Structure

Suc-YVAD-pNA
Suc-YVAD-pNA
CAS#208264-84-0

Theoretical Analysis

MedKoo Cat#: 574010

Name: Suc-YVAD-pNA

CAS#: 208264-84-0

Chemical Formula: C31H38N6O12

Exact Mass: 686.2548

Molecular Weight: 686.68

Elemental Analysis: C, 54.22; H, 5.58; N, 12.24; O, 27.96

Price and Availability

Size Price Availability Quantity
1mg USD 300.00
5mg USD 450.00
10mg USD 600.00
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Related CAS #
No Data
Synonym
Caspase-1 and Caspase-4 Chromogenic Substrate, Suc-YVAD-pNA, Suc-Tyr-Val-Ala-Asp-p-nitroanilide
IUPAC/Chemical Name
N-(3-carboxy-1-oxopropyl)-L-tyrosyl-L-valyl-L-alanyl-N-(4-nitrophenyl)-L-α-asparagine
InChi Key
APPSYVHVOCTIDH-ZBBFOALVSA-N
InChi Code
InChI=1S/C31H38N6O12/c1-16(2)27(36-30(46)22(34-24(39)12-13-25(40)41)14-18-4-10-21(38)11-5-18)31(47)32-17(3)28(44)35-23(15-26(42)43)29(45)33-19-6-8-20(9-7-19)37(48)49/h4-11,16-17,22-23,27,38H,12-15H2,1-3H3,(H,32,47)(H,33,45)(H,34,39)(H,35,44)(H,36,46)(H,40,41)(H,42,43)/t17-,22-,23-,27-/m0/s1
SMILES Code
O=C([C@H](CC(O)=O)NC([C@H](C)NC([C@H](C(C)C)NC([C@@H](NC(CCC(O)=O)=O)CC1=CC=C(O)C=C1)=O)=O)=O)NC2=CC=C([N+]([O-])=O)C=C2
Appearance
Solid powder
Purity
>95% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
λmax: 223, 317 nm
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Suc-YVAD-pNA is a colorimetric substrate for caspase-1/interleukin-1β-converting enzyme (ICE) and caspase-4.
In vitro activity:
To be determined
In vivo activity:
To be determined

Preparing Stock Solutions

The following data is based on the product molecular weight 686.68 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
To be determined
In vitro protocol:
To be determined
In vivo protocol:
To be determined
Margolin, N., Raybuck, S. A., Wilson, K. P., Chen, W., Fox, T., Gu, Y., & Livingston, D. J. (1997). Substrate and inhibitor specificity of interleukin-1β-converting enzyme and related caspases. Journal of Biological Chemistry, 272(11), 7223-7228. Falkenberg, F., Rahba, J., Fischer, D., Bott, M., Bongaerts, J., & Siegert, P. (2022). Biochemical characterization of a novel oxidatively stable, halotolerant, and high‐alkaline subtilisin from Alkalihalobacillus okhensis Kh10‐101T. FEBS Open Bio, 12(10), 1729-1746. Fu, Z., Akula, S., Qiao, C., Ryu, J., Chahal, G., de Garavilla, L., ... & Hellman, L. (2021). Duodenases are a small subfamily of ruminant intestinal serine proteases that have undergone a remarkable diversification in cleavage specificity. PLoS One, 16(5), e0252624.

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RM-5038

RM-5038