Z-LRGG-AMC TFA is a fluorogenic substrate for isopeptidase T. Upon enzymatic cleavage by isopeptidase T, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify isopeptidase T activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.
MedKoo Cat#: 573998
Name: Z-LRGG-AMC TFA
CAS#: 167698-68-2 (free base)
Chemical Formula: C36H45F3N8O10
Exact Mass: 0.0000
Molecular Weight: 806.80
Elemental Analysis: C, 53.59; H, 5.62; F, 7.06; N, 13.89; O, 19.83
The following data is based on the product molecular weight 806.80 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |