Methylthialazole Tetrazolium, also known as MTT or Tetrazolium Bromide, is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells.1 It is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD(P)H-oxidoreductases. It is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.
MedKoo Cat#: 330214
Name: Methylthialazole Tetrazolium
CAS#: 298-93-1
Chemical Formula: C18H16BrN5S
Exact Mass: 0.0000
Molecular Weight: 414.33
Elemental Analysis: C, 52.18; H, 3.89; Br, 19.29; N, 16.90; S, 7.74
The following data is based on the product molecular weight 414.33 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
