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MedKoo Cat#: 585074 | Name: HODHBt
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

HODHBt is a STAT5-SUMO protein-protein interaction inhibitor.

Chemical Structure

HODHBt
HODHBt
CAS#28230-32-2

Theoretical Analysis

MedKoo Cat#: 585074

Name: HODHBt

CAS#: 28230-32-2

Chemical Formula: C7H5N3O2

Exact Mass: 163.0382

Molecular Weight: 163.14

Elemental Analysis: C, 51.54; H, 3.09; N, 25.76; O, 19.61

Price and Availability

Size Price Availability Quantity
100mg USD 250.00 2 Weeks
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Related CAS #
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Synonym
HODHBt
IUPAC/Chemical Name
3-Hydroxy-1,2,3-benzotriazin-4(3H)-one
InChi Key
HJBLUNHMOKFZQX-UHFFFAOYSA-N
InChi Code
InChI=1S/C7H5N3O2/c11-7-5-3-1-2-4-6(5)8-9-10(7)12/h1-4,12H
SMILES Code
O=C1N(O)N=NC2=CC=CC=C12
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
HODHBt is a STAT5-SUMO protein-protein interaction inhibitor.
In vitro activity:
As expected, HODHBt caused a significant increase in p24 positive cells over IL-2-treated cells, albeit at a lower level than cells induced with αCD3/αCD28 (Figure 2B). These results demonstrate that HODHBt-induced HIV-1 reactivation is capable of reducing the latent reservoir in vitro. Reference: Cell Rep. 2017 Jan 31;18(5):1324-1334. https://pubmed.ncbi.nlm.nih.gov/28147284/
In vivo activity:
TBD
Solvent mg/mL mM
Solubility
DMSO 16.3 100.00
Ethanol 8.2 50.00
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 163.14 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Macedo AB, Levinger C, Nguyen BN, Richard J, Gupta M, Cruz CRY, Finzi A, Chiappinelli KB, Crandall KA, Bosque A. The HIV Latency Reversal Agent HODHBt Enhances NK Cell Effector and Memory-Like Functions by Increasing Interleukin-15-Mediated STAT Activation. J Virol. 2022 Aug 10;96(15):e0037222. doi: 10.1128/jvi.00372-22. Epub 2022 Jul 14. PMID: 35867565; PMCID: PMC9364794. 2. Bosque A, Nilson KA, Macedo AB, Spivak AM, Archin NM, Van Wagoner RM, Martins LJ, Novis CL, Szaniawski MA, Ireland CM, Margolis DM, Price DH, Planelles V. Benzotriazoles Reactivate Latent HIV-1 through Inactivation of STAT5 SUMOylation. Cell Rep. 2017 Jan 31;18(5):1324-1334. doi: 10.1016/j.celrep.2017.01.022. PMID: 28147284; PMCID: PMC5461578.
In vitro protocol:
1. Macedo AB, Levinger C, Nguyen BN, Richard J, Gupta M, Cruz CRY, Finzi A, Chiappinelli KB, Crandall KA, Bosque A. The HIV Latency Reversal Agent HODHBt Enhances NK Cell Effector and Memory-Like Functions by Increasing Interleukin-15-Mediated STAT Activation. J Virol. 2022 Aug 10;96(15):e0037222. doi: 10.1128/jvi.00372-22. Epub 2022 Jul 14. PMID: 35867565; PMCID: PMC9364794. 2. Bosque A, Nilson KA, Macedo AB, Spivak AM, Archin NM, Van Wagoner RM, Martins LJ, Novis CL, Szaniawski MA, Ireland CM, Margolis DM, Price DH, Planelles V. Benzotriazoles Reactivate Latent HIV-1 through Inactivation of STAT5 SUMOylation. Cell Rep. 2017 Jan 31;18(5):1324-1334. doi: 10.1016/j.celrep.2017.01.022. PMID: 28147284; PMCID: PMC5461578.
In vivo protocol:
TBD
Bosque et al (2017) Benzotriazoles reactivate latent HIV-1 through inactivation of STAT5 SUMOylation. Cell Rep. 18 1324 PMID: 28147284