INH14 | CAS# 200134-22-1 | TLR Inflammatory Response Inhibitor

MedKoo Cat#: 565331 | Name: INH14

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

INH14 is an inhibitor of the IKKα/β-dependent TLR inflammatory response.

Chemical Structure

INH14

CAS#200134-22-1

Theoretical Analysis

MedKoo Cat#: 565331

Name: INH14

CAS#: 200134-22-1

Chemical Formula: C15H16N2O

Exact Mass: 240.1263

Molecular Weight: 240.31

Elemental Analysis: C, 74.97; H, 6.71; N, 11.66; O, 6.66

Price and Availability

Size	Price	Availability	Quantity
5mg	USD 250.00	2 Weeks
10mg	USD 400.00	2 Weeks

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Synonym

INH14; INH 14; INH-14

IUPAC/Chemical Name

N-(4-Ethylphenyl)-N'-phenylurea

InChi Key

CPCZNJSFVOOZOG-UHFFFAOYSA-N

InChi Code

InChI=1S/C15H16N2O/c1-2-12-8-10-14(11-9-12)17-15(18)16-13-6-4-3-5-7-13/h3-11H,2H2,1H3,(H2,16,17,18)

SMILES Code

O=C(NC1=CC=CC=C1)NC2=CC=C(CC)C=C2

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

INH14 is a cell permeable inhibitor of IKKα/IKKβ, with IC50s of 8.97 and 3.59 μM, respectively. INH14 inhibits the IKKα/β-dependent TLR inflammatory response. INH14 also inhibits downstream of TAK1/TAB1 and NF-kB pathways. Anti-inflammatory and anti-cancer activity.

In vitro activity:

The reduction in TNFα production by other MyD88‐dependent TLRs after treatment with INH14 has also been confirmed (i.e., TLR7/8, TLR5; Figure S1 in the Supporting Information). Additionally, this study found a decrease in the production of TNFα by human monocytes after INH14 treatment if they were stimulated with P3 (reduced to 60.43 %), LPS (72.62 %), or IL‐1 (73.30 %; Figure 2 B). Reference: Chembiochem. 2019 Mar 1;20(5):710-717. https://pubmed.ncbi.nlm.nih.gov/30447158/

In vivo activity:

Mice treated with INH14 had a decreased level of TNFα, in comparison with the control group (from (231.1±21.3) to (115.8±30.61) pg mL−1). Thus, the inhibition of IKKα/β by INH14 in vivo leads to a decrease in TNFα production after lipopeptide injection. During these experiments, this study did not observe pathological effects in animals treated with the compound (e.g., weight loss, abnormal movements, dyspnoea). Reference: Chembiochem. 2019 Mar 1;20(5):710-717. https://pubmed.ncbi.nlm.nih.gov/30447158/

	Solvent	mg/mL	mM
Solubility
	DMSO	86.5	359.96
	Ethanol	18.0	74.90

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 240.31 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Drexel M, Kirchmair J, Santos-Sierra S. INH14, a Small-Molecule Urea Derivative, Inhibits the IKKα/β-Dependent TLR Inflammatory Response. Chembiochem. 2019 Mar 1;20(5):710-717. doi: 10.1002/cbic.201800647. Epub 2019 Jan 29. PMID: 30447158; PMCID: PMC6680106.

In vitro protocol:

In vivo protocol:

1: Drexel M, Kirchmair J, Santos-Sierra S. INH14, a Small-Molecule Urea Derivative, Inhibits the IKKα/β-Dependent TLR Inflammatory Response. Chembiochem. 2019 Mar 1;20(5):710-717. doi: 10.1002/cbic.201800647. Epub 2019 Jan 29. PubMed PMID: 30447158.