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MedKoo Cat#: 584954 | Name: LNT 1
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

LNT 1, also known as FEN1-IN-1, is a potent flap endonuclease 1 (FEN1) inhibitor that induces DNA damage response.

Chemical Structure

LNT 1
LNT 1
CAS#824983-91-7

Theoretical Analysis

MedKoo Cat#: 584954

Name: LNT 1

CAS#: 824983-91-7

Chemical Formula: C15H12N2O5S

Exact Mass: 332.0467

Molecular Weight: 332.33

Elemental Analysis: C, 54.21; H, 3.64; N, 8.43; O, 24.07; S, 9.65

Price and Availability

Size Price Availability Quantity
50mg USD 350.00 2 weeks
100mg USD 550.00 2 Weeks
250mg USD 950.00 2 Weeks
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Related CAS #
No Data
Synonym
LNT 1; LNT1; LNT-1; FEN1-IN-1; FEN1- N-1; FEN1-IN1;
IUPAC/Chemical Name
1-[(2,3-Dihydro-1,4-benzodioxin-2-yl)methyl]-3-hydroxythieno[3,2-d]pyrimidine-2,4(1H,3H)-dione
InChi Key
MXQGCMQXTPTJJT-UHFFFAOYSA-N
InChi Code
InChI=1S/C15H12N2O5S/c18-14-13-10(5-6-23-13)16(15(19)17(14)20)7-9-8-21-11-3-1-2-4-12(11)22-9/h1-6,9,20H,7-8H2
SMILES Code
O=C1N(CC2OC3=CC=CC=C3OC2)C(C=CS4)=C4C(N1O)=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
FEN1-IN-1 (compound 1) is a flap endonuclease 1 (FEN1) inhibitor.
In vitro activity:
High concentrations of compound 1 (FEN1-IN-1) proved cytotoxic towards SW620 cells with an EC50 of 11 μM (Figure 5d), but HeLa cells stably expressing hFEN1-shRNA were 70% viable at 20 μM 1 (Figure 5e; purple curve). When treated with sub-lethal doses of 1, SW620 cells showed evidence of an induced DNA damage response (Figure 5h and Supplementary Figure 18) at concentrations consistent with the EC50 for target engagement observed by CETSA. Reference: Nat Chem Biol. 2016 Oct;12(10):815-21. https://pubmed.ncbi.nlm.nih.gov/27526030/
In vivo activity:
TBD
Solvent mg/mL mM
Solubility
DMF 15.0 45.14
DMSO 92.2 277.46
Ethanol 4.0 12.04
PBS (pH 7.2) 0.1 0.33
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 332.33 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Exell JC, Thompson MJ, Finger LD, Shaw SJ, Debreczeni J, Ward TA, McWhirter C, Siöberg CL, Martinez Molina D, Abbott WM, Jones CD, Nissink JW, Durant ST, Grasby JA. Cellularly active N-hydroxyurea FEN1 inhibitors block substrate entry to the active site. Nat Chem Biol. 2016 Oct;12(10):815-21. doi: 10.1038/nchembio.2148. Epub 2016 Aug 15. PMID: 27526030; PMCID: PMC5348030.
In vitro protocol:
1. Exell JC, Thompson MJ, Finger LD, Shaw SJ, Debreczeni J, Ward TA, McWhirter C, Siöberg CL, Martinez Molina D, Abbott WM, Jones CD, Nissink JW, Durant ST, Grasby JA. Cellularly active N-hydroxyurea FEN1 inhibitors block substrate entry to the active site. Nat Chem Biol. 2016 Oct;12(10):815-21. doi: 10.1038/nchembio.2148. Epub 2016 Aug 15. PMID: 27526030; PMCID: PMC5348030.
In vivo protocol:
TBD
1. Exell et al (2016) Cellularly active N-hydroxyurea FEN1 inhibitors block substrate entry to the active site. Nat.Chem.Bio. 12 815 PMID: 27526030 2. Ward et al (2017) Small molecule inhibitors uncover synthetic genetic interactions of human flap endonuclease 1 (FEN1) with DNA damage response genes. PLoS One 12 e0179278 PMID: 28628639