Copper probe CF4 or CF4 probe, is a fluorescent copper probe. CF4 is a Cu+-specific fluorescent sensor based on a rhodol dye scaffold that displays a tenfold increase in fluorescence in response to copper; is stable in a physiologically relevant pH regime between 6 and 8; has high copper selectivity, particularly over zinc and iron, as well as abundant cellular alkali and alkaline earth metals ; and has a 1:1 Cu+:probe binding stoichiometry and an apparent Kd of 2.9 × 10−13 M. This product is being supplied as solution at 100 mg/mL.
MedKoo Cat#: 564632
Name: Copper probe CF4
CAS#: 2365532-64-3
Chemical Formula: C38H47F3N2O2S4
Exact Mass: 748.2472
Molecular Weight: 749.04
Elemental Analysis: C, 60.93; H, 6.32; F, 7.61; N, 3.74; O, 4.27; S, 17.12
| Solvent | mg/mL | mM | comments | |
|---|---|---|---|---|
| Solubility | ||||
| DMSO | 125.0 | 166.88 |
The following data is based on the product molecular weight 749.04 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
