Synonym
Sulfo-Cy5-acid; Sulfo-Cy5 acid; Sulfo Cy5 acid; Sulfo-Cyanine5; Sulfo-Cyanine 5; Sulfo-Cyanine-5; CY-5; CY5; CY 5
IUPAC/Chemical Name
2-((1E,3E)-5-((E)-1-(5-carboxypentyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
InChi Key
HPICMEGAGMPYID-UHFFFAOYSA-N
InChi Code
InChI=1S/C33H40N2O8S2/c1-6-34-27-18-16-23(44(38,39)40)21-25(27)32(2,3)29(34)13-9-7-10-14-30-33(4,5)26-22-24(45(41,42)43)17-19-28(26)35(30)20-12-8-11-15-31(36)37/h7,9-10,13-14,16-19,21-22H,6,8,11-12,15,20H2,1-5H3,(H2-,36,37,38,39,40,41,42,43)
SMILES Code
CC(C)(C1=C2C=CC(S(=O)(O)=O)=C1)/C(N2CCCCCC(O)=O)=C\C=C\C=C\C3=[N+](C4=C(C3(C)C)C=C(S(=O)([O-])=O)C=C4)CC
Purity
>95% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
Preparing Stock Solutions
The following data is based on the
product
molecular weight
656.81
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1: Fothergill JW, Hernandez AC, Knowlton WB, Yurke B, Li L. Ab Initio Studies of Exciton Interactions of Cy5 Dyes. J Phys Chem A. 2018 Nov 21;122(46):8989-8997. doi: 10.1021/acs.jpca.8b05237. Epub 2018 Nov 9. PubMed PMID: 30380862.
2: Berlepsch HV, Böttcher C. Tubular J-aggregates of a new thiacarbocyanine Cy5 dye for the far-red spectral region - a spectroscopic and cryo-transmission electron microscopy study. Phys Chem Chem Phys. 2018 Jul 18;20(28):18969-18977. doi: 10.1039/c8cp03378a. PubMed PMID: 29975386.
3: Lee KH, Nam H, Won JS, Hwang JY, Jang HW, Lee SH, Joo KM. In Vivo Spinal Distribution of Cy5.5 Fluorescent Dye after Injection via the Lateral Ventricle and Cisterna Magna in Rat Model. J Korean Neurosurg Soc. 2018 Jul;61(4):434-440. doi: 10.3340/jkns.2017.0252. Epub 2018 Apr 18. PubMed PMID: 29660974; PubMed Central PMCID: PMC6046575.
4: Zasedateleva OA, Vasiliskov VA, Surzhikov SA, Kuznetsova VE, Shershov VE, Guseinov TO, Smirnov IP, Yurasov RA, Spitsyn MA, Chudinov AV. dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase. Nucleic Acids Res. 2018 Jul 6;46(12):e73. doi: 10.1093/nar/gky247. PubMed PMID: 29648660; PubMed Central PMCID: PMC6158613.
5: Miranda P, Oliveira LM, Weber G. Mesoscopic modelling of Cy3 and Cy5 dyes attached to DNA duplexes. Biophys Chem. 2017 Nov;230:62-67. doi: 10.1016/j.bpc.2017.08.007. Epub 2017 Sep 27. PubMed PMID: 28965786.
6: Kim Y, Seo HH, Jeong MS, Lee KH, Lee IH, So KA, Kim MK, Lee YK, Kim SA, Kim TJ. Method to minimize ozone effect on Cy5 fluorescent intensity in DNA microarrays. Anal Biochem. 2017 Dec 1;538:1-4. doi: 10.1016/j.ab.2017.09.001. Epub 2017 Sep 4. PubMed PMID: 28882748.
7: Jia X, Chen Q, Yang Y, Tang Y, Wang R, Xu Y, Zhu W, Qian X. FRET-Based Mito-Specific Fluorescent Probe for Ratiometric Detection and Imaging of Endogenous Peroxynitrite: Dyad of Cy3 and Cy5. J Am Chem Soc. 2016 Aug 31;138(34):10778-81. doi: 10.1021/jacs.6b06398. Epub 2016 Aug 22. PubMed PMID: 27517310.
8: Kretschy N, Sack M, Somoza MM. Sequence-Dependent Fluorescence of Cy3- and Cy5-Labeled Double-Stranded DNA. Bioconjug Chem. 2016 Mar 16;27(3):840-8. doi: 10.1021/acs.bioconjchem.6b00053. Epub 2016 Feb 26. PubMed PMID: 26895222; PubMed Central PMCID: PMC4796863.
