BTZO-1 | CAS# 99420-15-2 | ARE Activator

MedKoo Cat#: 564294 | Name: BTZO-1

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

BTZO-1 is an activator of the glutathione S-transferase Ya subunit (GST Ya) gene ARE by interacting with MIF.

Chemical Structure

BTZO-1

CAS#99420-15-2

Theoretical Analysis

MedKoo Cat#: 564294

Name: BTZO-1

CAS#: 99420-15-2

Chemical Formula: C13H8N2OS

Exact Mass: 240.0357

Molecular Weight: 240.28

Elemental Analysis: C, 64.98; H, 3.36; N, 11.66; O, 6.66; S, 13.34

Price and Availability

Size	Price	Availability
10mg	USD 250.00	2 weeks
25mg	USD 450.00	2 weeks
50mg	USD 800.00	2 Weeks

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Related CAS #

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Synonym

BTZO1; BTZO 1; BTZO-1

IUPAC/Chemical Name

2-Pyridin-2-yl-1,3-benzothiazin-4-one

InChi Key

GBAKVEWPYUIGHN-UHFFFAOYSA-N

InChi Code

InChI=1S/C13H8N2OS/c16-12-9-5-1-2-7-11(9)17-13(15-12)10-6-3-4-8-14-10/h1-8H

SMILES Code

O=C1N=C(C2=NC=CC=C2)SC3=CC=CC=C13

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.03.00

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

BTZO-1 binds to Macrophage migration inhibitory factor (MIF) with a Kd value of 68.6 nM, and its binding requires the N-terminal Pro1.

In vitro activity:

BTZO-1 binds to MIF with a K(d) of 68.6 nM, and its binding required the intact N-terminal Pro1. MIF, in the presence of BTZO-1, activated the glutathione S-transferase Ya subunit (GST Ya) gene ARE, whereas reduction of cellular MIF protein levels by siRNA suppressed BTZO-1-induced GST Ya expression. These results suggest that BTZO-1 activates the GST Ya gene ARE by interacting with MIF. Reference: Chem Biol. 2010 Dec 22;17(12):1282-94. https://pubmed.ncbi.nlm.nih.gov/21168764/

In vivo activity:

Addition of BTZO-1 during IVM of bovine oocytes improved their developmental competence in the term of improvement of blastocyst rates. Reference: Reprod Domest Anim. 2020 Aug;55(8):1021-1024. https://pubmed.ncbi.nlm.nih.gov/32473610/

	Solvent	mg/mL	mM
Solubility
	DMSO	7.7	32.09
	Ethanol	4.0	16.65

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 240.28 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Kimura H, Sato Y, Tajima Y, Suzuki H, Yukitake H, Imaeda T, Kajino M, Oki H, Takizawa M, Tanida S. BTZO-1, a cardioprotective agent, reveals that macrophage migration inhibitory factor regulates ARE-mediated gene expression. Chem Biol. 2010 Dec 22;17(12):1282-94. doi: 10.1016/j.chembiol.2010.10.011. PMID: 21168764. 2. Aboelenain M, El Domany WB, Eldoumani H, Khalil WA. Antioxidant response element activator, BTZO-1, improves the developmental competence of bovine oocytes. Reprod Domest Anim. 2020 Aug;55(8):1021-1024. doi: 10.1111/rda.13733. Epub 2020 Jun 12. PMID: 32473610.

In vitro protocol:

In vivo protocol:

1. Aboelenain M, El Domany WB, Eldoumani H, Khalil WA. Antioxidant response element activator, BTZO-1, improves the developmental competence of bovine oocytes. Reprod Domest Anim. 2020 Aug;55(8):1021-1024. doi: 10.1111/rda.13733. Epub 2020 Jun 12. PMID: 32473610.

1: Kimura H, Sato Y, Tajima Y, Suzuki H, Yukitake H, Imaeda T, Kajino M, Oki H, Takizawa M, Tanida S. BTZO-1, a cardioprotective agent, reveals that macrophage migration inhibitory factor regulates ARE-mediated gene expression. Chem Biol. 2010 Dec 22;17(12):1282-94. doi: 10.1016/j.chembiol.2010.10.011. PubMed PMID: 21168764. 2: Yukitake H, Takizawa M, Kimura H. Macrophage Migration Inhibitory Factor as an Emerging Drug Target to Regulate Antioxidant Response Element System. Oxid Med Cell Longev. 2017;2017:8584930. doi: 10.1155/2017/8584930. Epub 2017 Jan 16. Review. PubMed PMID: 28191280; PubMed Central PMCID: PMC5278225.