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MedKoo Cat#: 330154 | Name: Light Green SF Yellowish
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Light green SF yellowish (C.I. 42095) is a dye that is used for the preparation of a staining solution which is is widely used as a counterstain. for e.g. trichrome Masson-Goldner staining in histological sections or for Papanicolaou’s polychromatic staining in cytology of samples human origin

Chemical Structure

Light Green SF Yellowish
Light Green SF Yellowish
CAS#5141-20-8

Theoretical Analysis

MedKoo Cat#: 330154

Name: Light Green SF Yellowish

CAS#: 5141-20-8

Chemical Formula: C37H34N2Na2O9S3

Exact Mass:

Molecular Weight: 792.84

Elemental Analysis: C, 56.05; H, 4.32; N, 3.53; Na, 5.80; O, 18.16; S, 12.13

Price and Availability

Size Price Availability Quantity
5g USD 250.00 2 Weeks
25g USD 450.00 2 Weeks
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Synonym
Light Green SF Yellowish; Acid Green 5; C.I. 42095; C.I. Acid Green 5;
IUPAC/Chemical Name
sodium 3-((ethyl((1E,4E)-4-((4-(ethyl(3-sulfonatobenzyl)amino)phenyl)(4-sulfonatophenyl)methylene)cyclohexa-2,5-dien-1-ylidene)ammonio)methyl)benzenesulfonate
InChi Key
DGOBMKYRQHEFGQ-UHFFFAOYSA-L
InChi Code
InChI=1S/C37H36N2O9S3.2Na/c1-3-38(25-27-7-5-9-35(23-27)50(43,44)45)32-17-11-29(12-18-32)37(31-15-21-34(22-16-31)49(40,41)42)30-13-19-33(20-14-30)39(4-2)26-28-8-6-10-36(24-28)51(46,47)48;;/h5-24H,3-4,25-26H2,1-2H3,(H2-,40,41,42,43,44,45,46,47,48);;/q;2*+1/p-2
SMILES Code
O=S(C1=CC=CC(C/[N+](CC)=C2C=C/C(C=C\2)=C(C3=CC=C(N(CC)CC4=CC=CC(S(=O)([O-])=O)=C4)C=C3)/C5=CC=C(S(=O)([O-])=O)C=C5)=C1)([O-])=O.[Na+].[Na+]
Appearance
Dark Red to Purple Solid powder
Purity
>70% dye content (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in water (200mg/ mL)
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
3204 19 00
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Light green SF yellowish (Acid Green 5) is a triarylmethane dye.
In vitro activity:
The remaining two potential inhibitors were light green SF yellowish (45% decrease) and β-cyclodextrin (β-CD, 75% decrease). Dose-response analyses confirmed both of these excipients as modest inhibitors of digoxin flux, with IC50 estimates of 168 μM (95% CI, 118–251 μM, Figure 3D) and 204 μM (95% CI, 5.9–1745 μM, Figure 3E) for β-CD and light green SF yellowish, respectively (Figures 3D and 3E). Reference: AAPS J. 2021 Sep 15;23(5):106. https://pubmed.ncbi.nlm.nih.gov/34528148/
In vivo activity:
Four dyes in different solutions (light green SF yellowish [LGSF]: 2%; copper(II) phthalocyanine-tetrasulfonic acid [E68]: 2% and 0.5%; bromophenol blue [BPB]: 2%, 1%, and 0.2%; and Chicago blue [CB]: 2% and 0.5%) were included in this investigation. After triamcinolone-assisted vitrectomy on 10 porcine eyes in vivo, the dyes were first injected into the air-filled vitreous cavity. No staining of the retinal surface but of the vitreous was seen after application of LGSF 2%. Reference: Retina. 2006 Jul-Aug;26(6):673-8. https://pubmed.ncbi.nlm.nih.gov/16829811/

Preparing Stock Solutions

The following data is based on the product molecular weight 792.84 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Bajaj R, Chong LB, Zou L, Tsakalozou E, Ni Z, Giacomini KM, Kroetz DL. Interaction of Commonly Used Oral Molecular Excipients with P-glycoprotein. AAPS J. 2021 Sep 15;23(5):106. doi: 10.1208/s12248-021-00631-8. PMID: 34528148; PMCID: PMC9148196. 2. Haddad A, Merouani S, Hannachi C, Hamdaoui O, Hamrouni B. Intensification of light green SF yellowish (LGSFY) photodegradion in water by iodate ions: Iodine radicals implication in the degradation process and impacts of water matrix components. Sci Total Environ. 2019 Feb 20;652:1219-1227. doi: 10.1016/j.scitotenv.2018.10.183. Epub 2018 Oct 15. PMID: 30586808. 3. Haritoglou C, Tadayoni R, May CA, Gass CA, Freyer W, Priglinger SG, Kampik A. Short-term in vivo evaluation of novel vital dyes for intraocular surgery. Retina. 2006 Jul-Aug;26(6):673-8. doi: 10.1097/01.iae.0000236505.42892.54. PMID: 16829811.
In vitro protocol:
1. Bajaj R, Chong LB, Zou L, Tsakalozou E, Ni Z, Giacomini KM, Kroetz DL. Interaction of Commonly Used Oral Molecular Excipients with P-glycoprotein. AAPS J. 2021 Sep 15;23(5):106. doi: 10.1208/s12248-021-00631-8. PMID: 34528148; PMCID: PMC9148196. 2. Haddad A, Merouani S, Hannachi C, Hamdaoui O, Hamrouni B. Intensification of light green SF yellowish (LGSFY) photodegradion in water by iodate ions: Iodine radicals implication in the degradation process and impacts of water matrix components. Sci Total Environ. 2019 Feb 20;652:1219-1227. doi: 10.1016/j.scitotenv.2018.10.183. Epub 2018 Oct 15. PMID: 30586808.
In vivo protocol:
1. Haritoglou C, Tadayoni R, May CA, Gass CA, Freyer W, Priglinger SG, Kampik A. Short-term in vivo evaluation of novel vital dyes for intraocular surgery. Retina. 2006 Jul-Aug;26(6):673-8. doi: 10.1097/01.iae.0000236505.42892.54. PMID: 16829811.