Synonym
FiVe1; FiVe 1; FiVe-1
IUPAC/Chemical Name
8-Chloro-4-(4-(3-chlorophenyl)piperazin-1-yl)cinnoline
InChi Key
DZGXRHIQXADSDH-UHFFFAOYSA-N
InChi Code
InChI=1S/C18H16Cl2N4/c19-13-3-1-4-14(11-13)23-7-9-24(10-8-23)17-12-21-22-18-15(17)5-2-6-16(18)20/h1-6,11-12H,7-10H2
SMILES Code
ClC1=CC(N2CCN(C3=C4C=CC=C(Cl)C4=NN=C3)CC2)=CC=C1
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
More Info
FiVe1 targets the intermediate filament and mesenchymal marker vimentin (VIM) in a mode which promotes VIM disorganization and phosphorylation during metaphase, ultimately leading to mitotic catastrophe, multinucleation, and the loss of stemness.
Preparing Stock Solutions
The following data is based on the
product
molecular weight
359.25
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1: Bollong MJ, Pietilä M, Pearson AD, Sarkar TR, Ahmad I, Soundararajan R, Lyssiotis CA, Mani SA, Schultz PG, Lairson LL. A vimentin binding small molecule leads to mitotic disruption in mesenchymal cancers. Proc Natl Acad Sci U S A. 2017 Nov 14;114(46):E9903-E9912. doi: 10.1073/pnas.1716009114. Epub 2017 Oct 30. PubMed PMID: 29087350; PubMed Central PMCID: PMC5699095.
2: Li M, Zhang YX, Zhang Z, Zhou XY, Zuo XL, Cong Y, Li YQ. Endomicroscopy Will Track Injected Mesenchymal Stem Cells in Rat Colitis Models. Inflamm Bowel Dis. 2015 Sep;21(9):2068-77. doi: 10.1097/MIB.0000000000000458. PubMed PMID: 25993690.
3: Kovács T, Varga G, Erces D, Tőkés T, Tiszlavicz L, Ghyczy M, Vécsei L, Boros M, Kaszaki J. [Comparative study of novel therapeutic possibilities in animal experimental model of inflammatory bowel disease]. Magy Seb. 2012 Aug;65(4):191-7. doi: 10.1556/MaSeb.65.2012.4.4. Hungarian. PubMed PMID: 22940387.
4: Goetz M, Ansems JV, Galle PR, Schuchmann M, Kiesslich R. In vivo real-time imaging of the liver with confocal endomicroscopy permits visualization of the temporospatial patterns of hepatocyte apoptosis. Am J Physiol Gastrointest Liver Physiol. 2011 Nov;301(5):G764-72. doi: 10.1152/ajpgi.00175.2011. Epub 2011 Jul 21. PubMed PMID: 21778462.
5: Goetz M, Toermer T, Vieth M, Dunbar K, Hoffman A, Galle PR, Neurath MF, Delaney P, Kiesslich R. Simultaneous confocal laser endomicroscopy and chromoendoscopy with topical cresyl violet. Gastrointest Endosc. 2009 Nov;70(5):959-68. doi: 10.1016/j.gie.2009.04.016. PubMed PMID: 19595315.
