Synonym
Biebrich scarlet; NSC 79710; NSC-79710; NSC79710
IUPAC/Chemical Name
Benzenesulfonic acid, 2-((2-hydroxy-1-naphthalenyl)azo)-5-((4-sulfophenyl)azo)-, disodium salt
InChi Key
VVAVKBBTPWYADW-RVTJCSDESA-L
InChi Code
InChI=1S/C22H16N4O7S2.2Na/c27-20-12-5-14-3-1-2-4-18(14)22(20)26-25-19-11-8-16(13-21(19)35(31,32)33)24-23-15-6-9-17(10-7-15)34(28,29)30;;/h1-13,27H,(H,28,29,30)(H,31,32,33);;/q;2*+1/p-2/b24-23+,26-25+;;
SMILES Code
O=S(C1=CC(/N=N/C2=CC=C(S(=O)([O-])=O)C=C2)=CC=C1/N=N/C3=C4C=CC=CC4=CC=C3O)([O-])=O.[Na+].[Na+]
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
Preparing Stock Solutions
The following data is based on the
product
molecular weight
556.47
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
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2: Mlynek ML. Comparative investigations on the specificity of Adams' reaction and the Biebrich scarlet stain for the demonstration of eosinophilic granules. Klin Wochenschr. 1985 Jul 15;63(14):646-7. PubMed PMID: 2411996.
3: Hague DN, Henshaw JS, John VA, Pooley MJ, Chock PB. Dimerization of Biebrich scarlet and the monomer-alha-chymorypsin interaction. Nature. 1971 Jan 15;229(5281):190-1. PubMed PMID: 4923262.
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19: Garvey W, Fathi A, Bigelow F, Carpenter B, Jimenez C. A combined elastic, fibrin and collagen stain. Stain Technol. 1987 Nov;62(6):365-8. PubMed PMID: 2448920.
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