MedKoo Cat#: 598649 | Name: Leucogentian violet
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Leucogentian violet is a cationic triarylmethane dye. LCV is a reduced form of gentian violet (crystal violet) and thus, it is colorless. It has an affinity for both cellulosic and proteinaceous materials. LCV and hydrogen peroxide react with the hemoglobin in blood and turn the blood impression to purple/violet color. Therefore, the dye is widely used to stain blood residue on both porous and non-porous materials.

Chemical Structure

Leucogentian violet
Leucogentian violet
CAS#603-48-5

Theoretical Analysis

MedKoo Cat#: 598649

Name: Leucogentian violet

CAS#: 603-48-5

Chemical Formula: C25H31N3

Exact Mass: 373.2518

Molecular Weight: 373.54

Elemental Analysis: C, 80.39; H, 8.37; N, 11.25

Price and Availability

Size Price Availability Quantity
25g USD 370.00
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Related CAS #
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Synonym
Leucogentian violet; AI3-19978; C.I. Basic Violet 3, leuco; NSC 7338; NSC-7338; NSC7338;
IUPAC/Chemical Name
4,4',4''-methanetriyltris(N,N-dimethylaniline)
InChi Key
OAZWDJGLIYNYMU-UHFFFAOYSA-N
InChi Code
InChI=1S/C25H31N3/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6/h7-18,25H,1-6H3
SMILES Code
CN(C)C1=CC=C(C(C2=CC=C(C=C2)N(C)C)C3=CC=C(C=C3)N(C)C)C=C1
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
More Info
Product Data
Biological target:
Leucocrystal violet is a triphenylmethane dye which can be used to detect antimony in environmental and biological samples using spectrophotometric techniques.
In vitro activity:
TBD
In vivo activity:
TBD
Solvent mg/mL mM
Solubility
DMSO 12.5 33.46
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 373.54 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
TBD
In vitro protocol:
TBD
In vivo protocol:
TBD
1: McDonald JJ, Cerniglia CE. Biotransformation of gentian violet to leucogentian violet by human, rat, and chicken intestinal microflora. Drug Metab Dispos. 1984 May-Jun;12(3):330-6. PubMed PMID: 6145560. 2: Zhao J, Wei D, Yang Y. Magnetic solid-phase extraction for determination of the total malachite green, gentian violet and leucomalachite green, leucogentian violet in aquaculture water by high-performance liquid chromatography with fluorescence detection. J Sep Sci. 2016 Jun;39(12):2347-55. doi: 10.1002/jssc.201501363. Epub 2016 May 31. PubMed PMID: 27120413. 3: Doerge DR, Churchwell MI, Rushing LG, Bajic S. Confirmation of gentian violet and its metabolite leucogentian violet in catfish muscle using liquid chromatography combined with atmospheric pressure ionization mass spectrometry. Rapid Commun Mass Spectrom. 1996;10(12):1479-84. PubMed PMID: 8885419. 4: Rushing LG, Webb SF, Thompson HC Jr. Determination of leucogentian violet and gentian violet in catfish tissue by high-performance liquid chromatography with visible detection. J Chromatogr B Biomed Appl. 1995 Dec 1;674(1):125-31. PubMed PMID: 8749260. 5: Roybal JE, Munns RK, Hurlbut JA, Shimoda W. Determination of gentian violet, its demethylated metabolites, and leucogentian violet in chicken tissue by liquid chromatography with electrochemical detection. J Assoc Off Anal Chem. 1990 Nov-Dec;73(6):940-6. PubMed PMID: 2289926. 6: Schermerhorn PG, Munns RK. Determination of leucogentian violet in chicken fat by liquid chromatography with electrochemical and ultraviolet detection: interlaboratory study. J AOAC Int. 1994 Nov-Dec;77(6):1454-60. PubMed PMID: 7819753. 7: Roybal JE, Munns RK, Hurlbut JA, Shimoda W. High-performance liquid chromatography of gentian violet, its demethylated metabolites, leucogentian violet and methylene blue with electrochemical detection. J Chromatogr. 1989 Apr 21;467(1):259-66. PubMed PMID: 2753937. 8: Thompson HC Jr, Rushing LG, Gehring T, Lochmann R. Persistence of gentian violet and leucogentian violet in channel catfish (ictalurus punctatus) muscle after water-borne exposure. J Chromatogr B Biomed Sci Appl. 1999 Feb 19;723(1-2):287-91. PubMed PMID: 10080657. 9: Wilson RT, Wong J, Johnston J, Epstein R, Heller DN. Confirmation of leucogentian violet in chicken fat by gas chromatography/mass spectrometry. J AOAC Int. 1994 Sep-Oct;77(5):1137-42. PubMed PMID: 7950415. 10: Rushing LG, Thompson HC Jr. Simultaneous determination of malachite green, gentian violet and their leuco metabolites in catfish or trout tissue by high-performance liquid chromatography with visible detection. J Chromatogr B Biomed Sci Appl. 1997 Jan 24;688(2):325-30. PubMed PMID: 9061471. 11: Munns RK, Roybal JE, Hurlbut JA, Shimoda W. Rapid method for determination of leucogentian violet in chicken fat by liquid chromatography with electrochemical detection. J Assoc Off Anal Chem. 1990 Sep-Oct;73(5):705-8. PubMed PMID: 2272994. 12: Docampo R, Moreno SN. The metabolism and mode of action of gentian violet. Drug Metab Rev. 1990;22(2-3):161-78. Review. PubMed PMID: 2272286. 13: Long C, Mai Z, Yang Y, Zhu B, Xu X, Lu L, Zou X. Determination of multi-residue for malachite green, gentian violet and their metabolites in aquatic products by high-performance liquid chromatography coupled with molecularly imprinted solid-phase extraction. J Chromatogr A. 2009 Mar 20;1216(12):2275-81. doi: 10.1016/j.chroma.2009.01.047. Epub 2009 Jan 21. PubMed PMID: 19211106. 14: Wu X, Zhang G, Wu Y, Hou X, Yuan Z. Simultaneous determination of malachite green, gentian violet and their leuco-metabolites in aquatic products by high-performance liquid chromatography-linear ion trap mass spectrometry. J Chromatogr A. 2007 Nov 23;1172(2):121-6. Epub 2007 Aug 7. PubMed PMID: 17950300. 15: Li H, Kijak PJ. Development of a quantitative multiclass/multiresidue method for 21 veterinary drugs in shrimp. J AOAC Int. 2011 Mar-Apr;94(2):394-406. PubMed PMID: 21563672. 16: Turnipseed SB, Roybal JE, Rupp HS, Hurlbut JA, Long AR. Particle beam liquid chromatography-mass spectrometry of triphenylmethane dyes: application to confirmation of malachite green in incurred catfish tissue. J Chromatogr B Biomed Appl. 1995 Aug 4;670(1):55-62. PubMed PMID: 7493085.