MedKoo Biosciences, Inc.
Tel:   +1-919-636-5577    Fax:   +1-919-980-4831    Email:   sales@medkoo.com
Search
Login Register
Search
MedKoo Cat#: 598628 | Name: Kemptide
Featured

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Kemptide is a synthetic heptapeptide with the sequence LRRASLG, commonly used as a specific substrate for protein kinase A (PKA) in biochemical assays. Its bioactivity lies in its ability to be efficiently phosphorylated by PKA at the serine residue, allowing researchers to measure PKA activity in vitro. The mechanism of action involves mimicking natural PKA substrates, enabling quantitative assessment of kinase function in signal transduction studies.

Chemical Structure

Kemptide
Kemptide
CAS#65189-71-1

Theoretical Analysis

MedKoo Cat#: 598628

Name: Kemptide

CAS#: 65189-71-1

Chemical Formula: C32H61N13O9

Exact Mass: 771.4715

Molecular Weight: 771.92

Elemental Analysis: C, 49.79; H, 7.97; N, 23.59; O, 18.65

Price and Availability

Size Price Availability Quantity
5mg USD 335.00 2 Weeks
10mg USD 550.00 2 Weeks
25mg USD 950.00 2 Weeks
Bulk Inquiry
Buy Now
Add to Cart
Related CAS #
No Data
Synonym
Kemptide; Leu-arg-arg-ala-ser-leu-gly;
IUPAC/Chemical Name
L-leucyl-L-arginyl-L-arginyl-L-alanyl-L-seryl-L-leucylglycine
InChi Key
WIGDGIGALMYEBW-LLINQDLYSA-N
InChi Code
InChI=1S/C32H61N13O9/c1-16(2)12-19(33)26(50)42-21(9-7-11-39-32(36)37)29(53)43-20(8-6-10-38-31(34)35)28(52)41-18(5)25(49)45-23(15-46)30(54)44-22(13-17(3)4)27(51)40-14-24(47)48/h16-23,46H,6-15,33H2,1-5H3,(H,40,51)(H,41,52)(H,42,50)(H,43,53)(H,44,54)(H,45,49)(H,47,48)(H4,34,35,38)(H4,36,37,39)/t18-,19-,20-,21-,22-,23-/m0/s1
SMILES Code
O=C(O)CNC([C@H](CC(C)C)NC([C@H](CO)NC([C@H](C)NC([C@H](CCCNC(N)=N)NC([C@H](CCCNC(N)=N)NC([C@H](CC(C)C)N)=O)=O)=O)=O)=O)=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA).
In vitro activity:
Different types of intact cells catalyze the phosphorylation of Kemptide in the presence of extracellular ATP and cAMP with Km values of 3-4 microM for Kemptide. Kemptide phosphorylation was influenced by PKI, the inhibitory protein specific for cAMP-PK. Reference: J Biol Chem. 1989 Aug 25;264(24):14549-55. https://pubmed.ncbi.nlm.nih.gov/2547801/
In vivo activity:
The specific activities of soluble A-kinase isolated from guinea-pig lung parenchyma and from bovine tracheal smooth muscle were significantly (12-16-fold) greater when Kemptide was used as substrate when compared to histone IIa. Reference: Biochem Pharmacol. 1990 Jan 15;39(2):271-83. https://pubmed.ncbi.nlm.nih.gov/2154228/
Solvent mg/mL mM
Solubility
DMF 30.0 38.86
DMSO 25.0 32.39
PBS (pH 7.2) 10.0 0.13
Water 50.0 0.03
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 771.92 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Kübler D, Pyerin W, Bill O, Hotz A, Sonka J, Kinzel V. Evidence for ecto-protein kinase activity that phosphorylates Kemptide in a cyclic AMP-dependent mode. J Biol Chem. 1989 Aug 25;264(24):14549-55. PMID: 2547801. 2. Giembycz MA, Diamond J. Evaluation of kemptide, a synthetic serine-containing heptapeptide, as a phosphate acceptor for the estimation of cyclic AMP-dependent protein kinase activity in respiratory tissues. Biochem Pharmacol. 1990 Jan 15;39(2):271-83. doi: 10.1016/0006-2952(90)90026-h. PMID: 2154228.
In vitro protocol:
1. Kübler D, Pyerin W, Bill O, Hotz A, Sonka J, Kinzel V. Evidence for ecto-protein kinase activity that phosphorylates Kemptide in a cyclic AMP-dependent mode. J Biol Chem. 1989 Aug 25;264(24):14549-55. PMID: 2547801.
In vivo protocol:
1. Giembycz MA, Diamond J. Evaluation of kemptide, a synthetic serine-containing heptapeptide, as a phosphate acceptor for the estimation of cyclic AMP-dependent protein kinase activity in respiratory tissues. Biochem Pharmacol. 1990 Jan 15;39(2):271-83. doi: 10.1016/0006-2952(90)90026-h. PMID: 2154228.
1: Guo J, Yue L, Ning L, Han A, Wang J. Phosphopeptide-bridged NH2-TiO2-mediated carbon dots self-enhancing and electrochemiluminescence microsensors for label-free protein kinase A detection. Mikrochim Acta. 2024 Sep 25;191(10):622. doi: 10.1007/s00604-024-06711-8. PMID: 39320530. 2: Olivieri C, Wang Y, Walker C, Subrahmanian MV, Ha KN, Bernlohr D, Gao J, Camilloni C, Vendruscolo M, Taylor SS, Veglia G. The αC-β4 loop controls the allosteric cooperativity between nucleotide and substrate in the catalytic subunit of protein kinase A. Elife. 2024 Jun 24;12:RP91506. doi: 10.7554/eLife.91506. PMID: 38913408; PMCID: PMC11196109. 3: Novero AG, Curcio C, Steeman TJ, Binolfi A, Krapf D, Buffone MG, Krapf D, Stival C. A versatile kinase mobility shift assay (KiMSA) for PKA analysis and cyclic AMP detection in sperm physiology (and beyond). Front Cell Dev Biol. 2024 Feb 20;12:1356566. doi: 10.3389/fcell.2024.1356566. PMID: 38444827; PMCID: PMC10912184. 4: Kwantwi-Barima P, Garimella SVB, Attah IK, Ibrahim YM. Evaluating Ion Accumulation and Storage in Traveling Wave Based Structures for Lossless Ion Manipulations. J Am Soc Mass Spectrom. 2023 Dec 6;34(12):2849-2856. doi: 10.1021/jasms.3c00348. Epub 2023 Nov 20. PMID: 37985653; PMCID: PMC10832571. 5: Delva-Wiley J, Ekhator ES, Adams LL, Patwardhan S, Dong M, Newman RH. Redox Modification of PKA-Cα Differentially Affects Its Substrate Selection. Life (Basel). 2023 Aug 26;13(9):1811. doi: 10.3390/life13091811. PMID: 37763215; PMCID: PMC10532679. 6: Zhang Y, Meng X, Liu K. The modulation of cAMP/PKA pathway by asiaticoside ameliorates high glucose-induced inflammation and apoptosis of retinal pigment epithelial cells. J Bioenerg Biomembr. 2022 Feb;54(1):9-16. doi: 10.1007/s10863-021-09929-w. Epub 2022 Jan 17. PMID: 35038080. 7: Guevara A, Lugo C, Montilla AJ, Calabokis M, Ferreira J, Martínez JC, Bubis J. Cloning, expression, solubilization, and purification of a functionally active recombinant cAMP-dependent protein kinase catalytic subunit-like protein PKAC1 from Trypanosoma equiperdum. Protein Expr Purif. 2022 Apr;192:106041. doi: 10.1016/j.pep.2021.106041. Epub 2021 Dec 23. PMID: 34953978. 8: Kim AK, Wu HD, Inoue T. Synthetic design of farnesyl-electrostatic peptides for development of a protein kinase A membrane translocation switch. Sci Rep. 2021 Aug 12;11(1):16421. doi: 10.1038/s41598-021-95840-8. PMID: 34385501; PMCID: PMC8361095. 9: Cho H, Lee CS, Kim TH. Label-Free Assay of Protein Kinase A Activity and Inhibition Using a Peptide-Based Electrochemical Sensor. Biomedicines. 2021 Apr 13;9(4):423. doi: 10.3390/biomedicines9040423. PMID: 33924719; PMCID: PMC8069798. 10: Escalona JL, Bubis J. Reversible phosphorylation of a protein from Trypanosoma equiperdum that exhibits homology with the regulatory subunits of mammalian cAMP-dependent protein kinases. Biochimie. 2021 Feb;181:204-213. doi: 10.1016/j.biochi.2020.12.018. Epub 2020 Dec 31. PMID: 33388361. 11: Wang J, Liu X, Wang C, Liu D, Li F, Wang L, Liu S. An Integral Recognition and Signaling for Electrochemical Assay of Protein Kinase Activity and Inhibitor by Reduced Graphene Oxide-Polydopamine-Silver Nanoparticle-Ti4+ Nanocomposite. Front Bioeng Biotechnol. 2020 Nov 13;8:603083. doi: 10.3389/fbioe.2020.603083. PMID: 33282854; PMCID: PMC7691532. 12: Sun Y, Zhang Y, Zhang H, Liu M, Liu Y. Integrating Highly Efficient Recognition and Signal Transition of g-C3N4 Embellished Ti3C2 MXene Hybrid Nanosheets for Electrogenerated Chemiluminescence Analysis of Protein Kinase Activity. Anal Chem. 2020 Aug 4;92(15):10668-10676. doi: 10.1021/acs.analchem.0c01776. Epub 2020 Jul 14. PMID: 32600031. 13: Lee CS, Oh DE, Kim TH. Label-free assay of protein kinase A activity and inhibition in cancer cell using electrochemically-prepared AuNP/rGO nanohybrid electrode modified with C-Kemptide. Talanta. 2020 Aug 1;215:120899. doi: 10.1016/j.talanta.2020.120899. Epub 2020 Mar 4. PMID: 32312444. 14: Mountain GA, Keating CD. Formation of Multiphase Complex Coacervates and Partitioning of Biomolecules within them. Biomacromolecules. 2020 Feb 10;21(2):630-640. doi: 10.1021/acs.biomac.9b01354. Epub 2019 Nov 27. PMID: 31743027. 15: Araujo NA, Rincón M, Vonasek E, Calabokis M, Bubis J. Biochemical characterization of the cAMP-dependent protein kinase regulatory subunit-like protein from Trypanosoma equiperdum, detection of its inhibitory activity, and identification of potential interacting proteins. Biochimie. 2020 Jan;168:110-123. doi: 10.1016/j.biochi.2019.10.020. Epub 2019 Nov 5. PMID: 31704351. 16: Tian T , Yao Y , Yang B , Zhang K , Liu B . Ultrasensitive amplification- free detection of protein kinase based on catalyzed assembly and enumeration of gold nanoparticles. Chem Commun (Camb). 2019 Feb 21;55(17):2505-2508. doi: 10.1039/c9cc00131j. PMID: 30741307. 17: Chen Z, Liu Y, Hao L, Zhu Z, Li F, Liu S. Reduced Graphene Oxide-Zirconium Dioxide-Thionine Nanocomposite Integrating Recognition, Amplification, and Signaling for an Electrochemical Assay of Protein Kinase Activity and Inhibitor Screening. ACS Appl Bio Mater. 2018 Nov 19;1(5):1557-1565. doi: 10.1021/acsabm.8b00451. Epub 2018 Oct 22. PMID: 34996206. 18: Guevara A, Lugo C, Montilla AJ, Araujo NA, Calabokis M, Bubis J. Glucose deprivation activates a cAMP-independent protein kinase from Trypanosoma equiperdum. Parasitology. 2019 Apr;146(5):643-652. doi: 10.1017/S0031182018001920. Epub 2018 Nov 13. PMID: 30419978. 19: He S, Kyaw YME, Tan EKM, Bekale L, Kang MWC, Kim SS, Tan I, Lam KP, Kah JCY. Quantitative and Label-Free Detection of Protein Kinase A Activity Based on Surface-Enhanced Raman Spectroscopy with Gold Nanostars. Anal Chem. 2018 May 15;90(10):6071-6080. doi: 10.1021/acs.analchem.7b05417. Epub 2018 Apr 26. PMID: 29697974. 20: Luzi NM, Lyons CE, Peterson DL, Ellis KC. Kinetics and inhibition studies of the L205R mutant of cAMP-dependent protein kinase involved in Cushing's syndrome. FEBS Open Bio. 2018 Mar 11;8(4):606-613. doi: 10.1002/2211-5463.12396. PMID: 29632813; PMCID: PMC5881547.

View History

Hydrobentizide

Hydrobentizide