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MedKoo Cat#: 563389 | Name: MDL-12330A
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

MDL-12330A is an inhibitor of adenylyl cyclase. It acts by inhibiting cAMP and cGMP phosphodiesterases and blocking slow extracellular and store-operated Ca2+ entry into cells.

Chemical Structure

MDL-12330A
MDL-12330A
CAS#40297-09-4

Theoretical Analysis

MedKoo Cat#: 563389

Name: MDL-12330A

CAS#: 40297-09-4

Chemical Formula: C23H37ClN2

Exact Mass: 0.0000

Molecular Weight: 377.01

Elemental Analysis: C, 73.27; H, 9.89; Cl, 9.40; N, 7.43

Price and Availability

Size Price Availability Quantity
10mg USD 320.00 2 Weeks
25mg USD 600.00 2 Weeks
50mg USD 950.00 2 Weeks
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Synonym
MDL-12330A; MDL 12330A; MDL12330A; MDL-12330-A; MDL 12330 A;
IUPAC/Chemical Name
(Z)-N-((1S,2S)-2-phenylcyclopentyl)azacyclotridec-1-en-2-amine hydrochloride
InChi Key
CKOPQUCSDBVAQG-VROPFNGYSA-N
InChi Code
InChI=1S/C23H36N2.ClH/c1-2-4-6-11-18-23(24-19-12-7-5-3-1)25-22-17-13-16-21(22)20-14-9-8-10-15-20;/h8-10,14-15,21-22H,1-7,11-13,16-19H2,(H,24,25);1H/t21-,22-;/m0./s1
SMILES Code
[H]Cl.C1(/N[C@@H]2[C@H](C3=CC=CC=C3)CCC2)=N/CCCCCCCCCCC1
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
MDL 12330A hydrochloride is an inhibitor of adenylyl cyclase.
In vitro activity:
MDL-12,330A (10 µmol/l) potentiated insulin secretion to 1.7 times of control in the presence of 8.3 mmol/l glucose, while SQ 22536 did not show significant effect on insulin secretion. MDL-12,330A prolonged AP durations (APDs) by inhibiting voltage-dependent K(+) (KV) channels, leading to an increase in [Ca(2+)]i levels. Reference: PLoS One. 2013 Oct 29;8(10):e77934. https://pubmed.ncbi.nlm.nih.gov/24205033/
In vivo activity:
N-(cis-2-phenylcyclopentyl)azacyclotridecan-2-imine hydrochloride (RMI 12330A) inhibited cyclic AMP and cyclic GMP phosphodiesterase activities in kidney preparations from rat and mouse. RMI 12330A inhibited cyclic AMP phosphodiesterase activities in intact rat renal tubules. Reference: Biochim Biophys Acta. 1980 Jun 13;613(2):499-506. https://pubmed.ncbi.nlm.nih.gov/6256001/
Solvent mg/mL mM
Solubility
DMF 1.0 2.65
Ethanol 1.0 2.65
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 377.01 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Li X, Guo Q, Gao J, Yang J, Zhang W, Liang Y, Wu D, Liu Y, Weng J, Li Q, Zhang Y. The adenylyl cyclase inhibitor MDL-12,330A potentiates insulin secretion via blockade of voltage-dependent K(+) channels in pancreatic beta cells. PLoS One. 2013 Oct 29;8(10):e77934. doi: 10.1371/journal.pone.0077934. PMID: 24205033; PMCID: PMC3812155. 2. van Rossum DB, Patterson RL, Ma HT, Gill DL. Ca2+ entry mediated by store depletion, S-nitrosylation, and TRP3 channels. Comparison of coupling and function. J Biol Chem. 2000 Sep 15;275(37):28562-8. doi: 10.1074/jbc.M003147200. PMID: 10878007. 3. Hunt NH, Evans T. RMI 12330A, an inhibitor of cyclic nucleotide phosphodiesterases and adenylate cyclase in kidney preparations. Biochim Biophys Acta. 1980 Jun 13;613(2):499-506. doi: 10.1016/0005-2744(80)90105-9. PMID: 6256001. 4. Guellaen G, Mahu JL, Mavier P, Berthelot P, Hanoune J. RMI 12330 A, an inhibitor of adenylate cyclase in rat liver. Biochim Biophys Acta. 1977 Oct 13;484(2):465-75. doi: 10.1016/0005-2744(77)90102-4. PMID: 911855.
In vitro protocol:
1. Li X, Guo Q, Gao J, Yang J, Zhang W, Liang Y, Wu D, Liu Y, Weng J, Li Q, Zhang Y. The adenylyl cyclase inhibitor MDL-12,330A potentiates insulin secretion via blockade of voltage-dependent K(+) channels in pancreatic beta cells. PLoS One. 2013 Oct 29;8(10):e77934. doi: 10.1371/journal.pone.0077934. PMID: 24205033; PMCID: PMC3812155. 2. van Rossum DB, Patterson RL, Ma HT, Gill DL. Ca2+ entry mediated by store depletion, S-nitrosylation, and TRP3 channels. Comparison of coupling and function. J Biol Chem. 2000 Sep 15;275(37):28562-8. doi: 10.1074/jbc.M003147200. PMID: 10878007.
In vivo protocol:
1. Hunt NH, Evans T. RMI 12330A, an inhibitor of cyclic nucleotide phosphodiesterases and adenylate cyclase in kidney preparations. Biochim Biophys Acta. 1980 Jun 13;613(2):499-506. doi: 10.1016/0005-2744(80)90105-9. PMID: 6256001. 2. Guellaen G, Mahu JL, Mavier P, Berthelot P, Hanoune J. RMI 12330 A, an inhibitor of adenylate cyclase in rat liver. Biochim Biophys Acta. 1977 Oct 13;484(2):465-75. doi: 10.1016/0005-2744(77)90102-4. PMID: 911855.
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