MedKoo Cat#: 563271 | Name: Phox-I1
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Phox-I1 is an inhibitor of NOX2 targeting the interactive site of p67phox with Rac GTPase.

Chemical Structure

Phox-I1
Phox-I1
CAS#1388151-90-3

Theoretical Analysis

MedKoo Cat#: 563271

Name: Phox-I1

CAS#: 1388151-90-3

Chemical Formula: C23H19N3O4

Exact Mass: 401.1376

Molecular Weight: 401.42

Elemental Analysis: C, 68.82; H, 4.77; N, 10.47; O, 15.94

Price and Availability

Size Price Availability Quantity
5mg USD 305.00 2 Weeks
10mg USD 505.00 2 Weeks
25mg USD 900.00 2 Weeks
50mg USD 1,375.00 2 Weeks
100mg USD 2,350.00 2 Weeks
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Related CAS #
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Synonym
Phox-I1; Phox I1; PhoxI1;
IUPAC/Chemical Name
9-methoxy-5-(3-nitrophenyl)-2-phenyl-1,10b-dihydro-5H-benzo[e]pyrazolo[1,5-c][1,3]oxazine
InChi Key
VTKZDTUTWZFSRQ-UHFFFAOYSA-N
InChi Code
InChI=1S/C23H19N3O4/c1-29-18-10-11-22-19(13-18)21-14-20(15-6-3-2-4-7-15)24-25(21)23(30-22)16-8-5-9-17(12-16)26(27)28/h2-13,21,23H,14H2,1H3
SMILES Code
O=[N+](C1=CC(C2OC3=CC=C(OC)C=C3C4N2N=C(C5=CC=CC=C5)C4)=CC=C1)[O-]
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
More Info
Product Data
Biological target:
Phox-I1 is an inhibitor of NOX2 targeting the interactive site of p67phox with Rac GTPase.
In vitro activity:
Here, this study has performed rational design and in silico screen to identify a small molecule inhibitor, Phox-I1, targeting the interactive site of p67(phox) with Rac GTPase, which is a necessary step of the signaling leading to NOX2 activation. Phox-I1 binds to p67(phox) with a submicromolar affinity and abrogates Rac1 binding and is effective in inhibiting NOX2-mediated superoxide production dose-dependently in human and murine neutrophils without detectable toxicity. Reference: Chem Biol. 2012 Feb 24;19(2):228-42. https://pubmed.ncbi.nlm.nih.gov/22365606/
In vivo activity:
TBD

Preparing Stock Solutions

The following data is based on the product molecular weight 401.42 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
Bosco EE, Kumar S, Marchioni F, Biesiada J, Kordos M, Szczur K, Meller J, Seibel W, Mizrahi A, Pick E, Filippi MD, Zheng Y. Rational design of small molecule inhibitors targeting the Rac GTPase-p67(phox) signaling axis in inflammation. Chem Biol. 2012 Feb 24;19(2):228-42. doi: 10.1016/j.chembiol.2011.12.017. PMID: 22365606; PMCID: PMC3292765.
In vitro protocol:
Bosco EE, Kumar S, Marchioni F, Biesiada J, Kordos M, Szczur K, Meller J, Seibel W, Mizrahi A, Pick E, Filippi MD, Zheng Y. Rational design of small molecule inhibitors targeting the Rac GTPase-p67(phox) signaling axis in inflammation. Chem Biol. 2012 Feb 24;19(2):228-42. doi: 10.1016/j.chembiol.2011.12.017. PMID: 22365606; PMCID: PMC3292765.
In vivo protocol:
TBD
1: Bosco EE, Kumar S, Marchioni F, Biesiada J, Kordos M, Szczur K, Meller J, Seibel W, Mizrahi A, Pick E, Filippi MD, Zheng Y. Rational design of small molecule inhibitors targeting the Rac GTPase-p67(phox) signaling axis in inflammation. Chem Biol. 2012 Feb 24;19(2):228-42. doi: 10.1016/j.chembiol.2011.12.017. PubMed PMID: 22365606; PubMed Central PMCID: PMC3292765. 2: Jo H, Luo HR. Exploiting effectors of Rac GTPase. Chem Biol. 2012 Feb 24;19(2):169-71. doi: 10.1016/j.chembiol.2012.02.001. PubMed PMID: 22365599; PubMed Central PMCID: PMC3292760. Chocry M, Leloup L. The NADPH Oxidase Family and Its Inhibitors. Antioxid Redox Signal. 2020 Aug 10;33(5):332-353. doi: 10.1089/ars.2019.7915. Epub 2020 Jan 23. PMID: 31826639.