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MedKoo Cat#: 597666 | Name: L-NIO HCl
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

L-NIO HCl is a non-selective inhibitor of all NOS isoforms. The Ki values for nNOS (rat), eNOS (bovine), and iNOS (mouse) are 1.7, 3.9, and 3.9 µM, respectively. L-NIO inhibits endothelial-dependent relaxation induced by acetylcholine in rat aortic rings and hypotensive responses to acetylcholine and bradykinin.

Chemical Structure

L-NIO HCl
L-NIO HCl
CAS#36889-13-1 (HCl)

Theoretical Analysis

MedKoo Cat#: 597666

Name: L-NIO HCl

CAS#: 36889-13-1 (HCl)

Chemical Formula: C7H15N3O2

Exact Mass:

Molecular Weight: 173.22

Elemental Analysis: C, 48.54; H, 8.73; N, 24.26; O, 18.47

Price and Availability

Size Price Availability Quantity
50mg USD 315.00 2 Weeks
100mg USD 550.00 2 Weeks
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Related CAS #
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Synonym
L-NIO HCl; N(G)-Iminoethylornithine; N5-(1-Iminoethyl)-L-ornithine;
IUPAC/Chemical Name
(S)-5-acetimidamido-2-aminopentanoic acid
InChi Key
UYZFAUAYFLEHRC-LURJTMIESA-N
InChi Code
InChI=1S/C7H15N3O2/c1-5(8)10-4-2-3-6(9)7(11)12/h6H,2-4,9H2,1H3,(H2,8,10)(H,11,12)/t6-/m0/s1
SMILES Code
N[C@@H](CCCNC(C)=N)C(O)=O
Appearance
Solid powder
Purity
>95% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
L-NIO HCl is a non-selective inhibitor of all NOS isoforms.
In vitro activity:
Interestingly, L-NIO, which is a NOS3 inhibitor, confers post-ischemic protection to axon function by attenuating mitochondrial fission and preserving mitochondrial motility via conserving levels of the microtubular adaptor protein Miro-2. Reference: Neuromolecular Med. 2019 Dec;21(4):484-492. https://pubmed.ncbi.nlm.nih.gov/31152363/
In vivo activity:
This study examined resting state functional connectivity (RSFC) as a surrogate after a focal lesion in the lateral corpus callosum induced by stereotaxic injection of L-NIO in mice. L-NIO induced focal lesions in the corpus callosum. Interhemispheric homotopic connectivity decreased by up to 50% 24 h after L-NIO, partially sparing the visual cortex. Reference: Cereb Cortex. 2021 Oct 1;31(11):4958-4969. https://pubmed.ncbi.nlm.nih.gov/34037216/
Solvent mg/mL mM
Solubility
DMF 20.0 115.46
DMSO 20.0 115.46
Ethanol 20.0 115.46
PBS (pH 7.2) 10.0 57.73
Water 100.0 577.31
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 173.22 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Bastian C, Day J, Politano S, Quinn J, Brunet S, Baltan S. Preserving Mitochondrial Structure and Motility Promotes Recovery of White Matter After Ischemia. Neuromolecular Med. 2019 Dec;21(4):484-492. doi: 10.1007/s12017-019-08550-w. Epub 2019 May 31. PMID: 31152363; PMCID: PMC6884671. 2. Polonchuk L, Chabria M, Badi L, Hoflack JC, Figtree G, Davies MJ, Gentile C. Cardiac spheroids as promising in vitro models to study the human heart microenvironment. Sci Rep. 2017 Aug 1;7(1):7005. doi: 10.1038/s41598-017-06385-8. PMID: 28765558; PMCID: PMC5539326. 3. Aykan SA, Xie H, Lai JH, Zheng Y, Chung DY, Kura S, Anzabi M, Sugimoto K, McAllister LM, Yaseen MA, Boas DA, Whalen MJ, Sakadzic S, Ayata C. Focal Subcortical White Matter Lesions Disrupt Resting State Cortical Interhemispheric Functional Connectivity in Mice. Cereb Cortex. 2021 Oct 1;31(11):4958-4969. doi: 10.1093/cercor/bhab134. PMID: 34037216; PMCID: PMC8491690.
In vitro protocol:
1. Bastian C, Day J, Politano S, Quinn J, Brunet S, Baltan S. Preserving Mitochondrial Structure and Motility Promotes Recovery of White Matter After Ischemia. Neuromolecular Med. 2019 Dec;21(4):484-492. doi: 10.1007/s12017-019-08550-w. Epub 2019 May 31. PMID: 31152363; PMCID: PMC6884671. 2. Polonchuk L, Chabria M, Badi L, Hoflack JC, Figtree G, Davies MJ, Gentile C. Cardiac spheroids as promising in vitro models to study the human heart microenvironment. Sci Rep. 2017 Aug 1;7(1):7005. doi: 10.1038/s41598-017-06385-8. PMID: 28765558; PMCID: PMC5539326.
In vivo protocol:
1. Aykan SA, Xie H, Lai JH, Zheng Y, Chung DY, Kura S, Anzabi M, Sugimoto K, McAllister LM, Yaseen MA, Boas DA, Whalen MJ, Sakadzic S, Ayata C. Focal Subcortical White Matter Lesions Disrupt Resting State Cortical Interhemispheric Functional Connectivity in Mice. Cereb Cortex. 2021 Oct 1;31(11):4958-4969. doi: 10.1093/cercor/bhab134. PMID: 34037216; PMCID: PMC8491690.
1: Muzaffar S, Jeremy JY, Angelini GD, Stuart-Smith K, Shukla N. Role of the endothelium and nitric oxide synthases in modulating superoxide formation induced by endotoxin and cytokines in porcine pulmonary arteries. Thorax. 2003 Jul;58(7):598-604. PubMed PMID: 12832676; PubMed Central PMCID: PMC1746752. 2: Conant K, Ahmed U, Schwartz JP, Major EO. IFN-gamma inhibits AP-1 binding activity in human brain-derived cells through a nitric oxide dependent mechanism. J Neuroimmunol. 1998 Aug 1;88(1-2):39-44. PubMed PMID: 9688322.