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MedKoo Cat#: 563159 | Name: o-3M3FBS
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

o-3M3FBS is a negative control for m-3M3FBS, an activator of phospholipase C (PLC).

Chemical Structure

o-3M3FBS
o-3M3FBS
CAS#313981-55-4

Theoretical Analysis

MedKoo Cat#: 563159

Name: o-3M3FBS

CAS#: 313981-55-4

Chemical Formula: C16H16F3NO2S

Exact Mass: 343.0854

Molecular Weight: 343.36

Elemental Analysis: C, 55.97; H, 4.70; F, 16.60; N, 4.08; O, 9.32; S, 9.34

Price and Availability

Size Price Availability Quantity
5mg USD 250.00
10mg USD 390.00
50mg USD 830.00
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No Data
Synonym
o-3M3FBS; o 3M3FBS; o3M3FBS;
IUPAC/Chemical Name
2,4,6-Trimethyl-N-[2-(trifluoromethyl)phenyl]benzenesulfonamide
InChi Key
SKJJIFRWCCSXGL-UHFFFAOYSA-N
InChi Code
InChI=1S/C16H16F3NO2S/c1-10-8-11(2)15(12(3)9-10)23(21,22)20-14-7-5-4-6-13(14)16(17,18)19/h4-9,20H,1-3H3
SMILES Code
O=S(C1=C(C)C=C(C)C=C1C)(NC2=CC=CC=C2C(F)(F)F)=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
o-3M3FBS is the negative control of m-3M3FBS. o-3M3FBS inhibits inward and outward currents via mechanisms independent of PLC acting in an antagonistic manner.
In vitro activity:
The active compound 2,4,6-trimethyl-N-(meta-3-trifluoromethyl-phenyl)-benzenesulfonamide (m-3M3FBS) stimulated a transient intracellular calcium concentration ([Ca(2+)](i)) increase in neutrophils. Moreover, m-3M3FBS stimulated the formation of inositol phosphates in U937 cells, indicating that it stimulates PLC activity. This study also observed that m-3M3FBS stimulated PLC activity in vitro. Reference: Mol Pharmacol. 2003 May;63(5):1043-50. https://pubmed.ncbi.nlm.nih.gov/12695532/
In vivo activity:
This study examined the effects of m-3M3FBS and 2, 4, 6-trimethyl-N-(ortho-3-trifluoromethyl-phenyl)-benzenesulfonamide (o-3M3FBS), on murine colonic smooth muscle tissue and cells by performing conventional microelectrode recordings, isometric force measurements and patch clamp experiments. Application of m-3M3FBS decreased spontaneous contractility in murine colonic smooth muscle without affecting the resting membrane potential. Patch clamp studies revealed that delayed rectifier K(+) channels were reversibly inhibited by m-3M3FBS and o-3M3FBS. Reference: Eur J Pharmacol. 2010 Feb 25;628(1-3):187-94. https://pubmed.ncbi.nlm.nih.gov/19931239/
Solvent mg/mL mM
Solubility
DMF 30.0 87.37
DMSO 51.5 149.83
Ethanol 34.3 100.00
Ethanol:PBS (pH 7.2) (1:3) 0.3 0.73
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 343.36 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Chen WC, Chou CT, Liou WC, Liu SI, Lin KL, Lu T, Lu YC, Hsu SS, Tsai JY, Liao WC, Liang WZ, Jan CR. Rise of [Ca²⁺]i and apoptosis induced by M-3M3FBS in SCM1 human gastric cancer cells. Chin J Physiol. 2014 Feb 28;57(1):31-40. doi: 10.4077/CJP.2014.BAB141. PMID: 24621336. 2. Bae YS, Lee TG, Park JC, Hur JH, Kim Y, Heo K, Kwak JY, Suh PG, Ryu SH. Identification of a compound that directly stimulates phospholipase C activity. Mol Pharmacol. 2003 May;63(5):1043-50. doi: 10.1124/mol.63.5.1043. PMID: 12695532. 3. Kim SD, Kim HJ, Shim JW, Lee HY, Lee SK, Kwon S, Jung YS, Baek SH, Park JS, Zabel BA, Bae YS. Phospholipase C activator m-3M3FBS protects against morbidity and mortality associated with sepsis. J Immunol. 2012 Aug 15;189(4):2000-5. doi: 10.4049/jimmunol.1200635. Epub 2012 Jul 13. PMID: 22798676. 4. Dwyer L, Kim HJ, Koh BH, Koh SD. Phospholipase C-independent effects of 3M3FBS in murine colon. Eur J Pharmacol. 2010 Feb 25;628(1-3):187-94. doi: 10.1016/j.ejphar.2009.11.029. Epub 2009 Nov 18. PMID: 19931239; PMCID: PMC3152466.
In vitro protocol:
1. Chen WC, Chou CT, Liou WC, Liu SI, Lin KL, Lu T, Lu YC, Hsu SS, Tsai JY, Liao WC, Liang WZ, Jan CR. Rise of [Ca²⁺]i and apoptosis induced by M-3M3FBS in SCM1 human gastric cancer cells. Chin J Physiol. 2014 Feb 28;57(1):31-40. doi: 10.4077/CJP.2014.BAB141. PMID: 24621336. 2. Bae YS, Lee TG, Park JC, Hur JH, Kim Y, Heo K, Kwak JY, Suh PG, Ryu SH. Identification of a compound that directly stimulates phospholipase C activity. Mol Pharmacol. 2003 May;63(5):1043-50. doi: 10.1124/mol.63.5.1043. PMID: 12695532.
In vivo protocol:
1. Kim SD, Kim HJ, Shim JW, Lee HY, Lee SK, Kwon S, Jung YS, Baek SH, Park JS, Zabel BA, Bae YS. Phospholipase C activator m-3M3FBS protects against morbidity and mortality associated with sepsis. J Immunol. 2012 Aug 15;189(4):2000-5. doi: 10.4049/jimmunol.1200635. Epub 2012 Jul 13. PMID: 22798676. 2. Dwyer L, Kim HJ, Koh BH, Koh SD. Phospholipase C-independent effects of 3M3FBS in murine colon. Eur J Pharmacol. 2010 Feb 25;628(1-3):187-94. doi: 10.1016/j.ejphar.2009.11.029. Epub 2009 Nov 18. PMID: 19931239; PMCID: PMC3152466.
1: Szebenyi SA, Ogura T, Sathyanesan A, AlMatrouk AK, Chang J, Lin W. Increases in intracellular calcium via activation of potentially multiple phospholipase C isozymes in mouse olfactory neurons. Front Cell Neurosci. 2014 Oct 21;8:336. doi: 10.3389/fncel.2014.00336. eCollection 2014. PubMed PMID: 25374507; PubMed Central PMCID: PMC4204526. 2: Dwyer L, Kim HJ, Koh BH, Koh SD. Phospholipase C-independent effects of 3M3FBS in murine colon. Eur J Pharmacol. 2010 Feb 25;628(1-3):187-94. doi: 10.1016/j.ejphar.2009.11.029. Epub 2009 Nov 18. PubMed PMID: 19931239; PubMed Central PMCID: PMC3152466.

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