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MedKoo Cat#: 563138 | Name: RO8191
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

RO8191 is an activator of JAK/STAT that induces IFN-like signals. RO8191 reverses the effects of baricitinib.

Chemical Structure

RO8191
RO8191
CAS#691868-88-9

Theoretical Analysis

MedKoo Cat#: 563138

Name: RO8191

CAS#: 691868-88-9

Chemical Formula: C14H5F6N5O

Exact Mass: 373.0398

Molecular Weight: 373.21

Elemental Analysis: C, 45.06; H, 1.35; F, 30.54; N, 18.77; O, 4.29

Price and Availability

Size Price Availability Quantity
10mg USD 260.00 2 Weeks
25mg USD 530.00 2 Weeks
50mg USD 850.00 2 Weeks
100mg USD 1,350.00 2 Weeks
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No Data
Synonym
RO8191; RO-8191; RO 8191; RO4948191; RO 4948191; RO-4948191;
IUPAC/Chemical Name
8-(1,3,4-Oxadiazol-2-yl)-2,4-bis(trifluoromethyl)-imidazo[1,2-a][1,8]naphthyridine
InChi Key
GRHYZVJEXKTJOS-UHFFFAOYSA-N
InChi Code
InChI=1S/C14H5F6N5O/c15-13(16,17)7-3-9(14(18,19)20)23-11-6(7)1-2-10-22-8(4-25(10)11)12-24-21-5-26-12/h1-5H
SMILES Code
FC(C1=C2C=CC3=NC(C4=NN=CO4)=CN3C2=NC(C(F)(F)F)=C1)(F)F
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
RO8191 is an agonist of interferon (IFN) α receptor type 2 (IFNAR2) with antiviral activity (EC50 = 200 nM). It exhibits 1:1 binding to the IFNAR2 extracellular domain. RO8191 induces expression of IFN-stimulated genes, without induction of genes encoding inflammatory cytokines and chemokines, in HCV replicon cells, several cancer cell lines, and human primary hepatocytes. RO8191 induces expression of IFN-stimulated genes in vivo without induction of inflammatory cytokine and chemokine expression in mice. It also reduces HCV titer in HCV-infected humanized liver mice following oral administration.
In vitro activity:
RO8191 inhibited Chikungunya virus (CHIKV) infection in BHK-21 and Vero-E6 cells with a selectivity index (SI) of 12.3 and 37.3, respectively. RO8191 protected cells from CHIKV infection, inhibit entry by virucidal activity, and strongly impair post-entry steps of viral replication. Reference: Virus Res. 2023 Jan 15;324:199029. https://pubmed.ncbi.nlm.nih.gov/36565816/
In vivo activity:
Infectious prions are responsible for transmissible spongiform encephalopathies including Creutzfeldt-Jakob disease. In in vivo and ex vivo prion-infected models, treatment with RO8191 inhibited prion invasion and prolonged the survival period of infected mice. Reference: Brain. 2019 Apr 1;142(4):1035-1050. https://pubmed.ncbi.nlm.nih.gov/30753318/
Solvent mg/mL mM
Solubility
DMSO 1.9 5.00
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 373.21 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Ruiz UEA, Santos IA, Grosche VR, Fernandes RS, de Godoy AS, Torres JDA, Freire MCLC, Mesquita NCMR, Guevara-Vega M, Nicolau-Junior N, Sabino-Silva R, Mineo TWP, Oliva G, Jardim ACG. Imidazonaphthyridine effects on Chikungunya virus replication: Antiviral activity by dependent and independent of interferon type 1 pathways. Virus Res. 2023 Jan 15;324:199029. doi: 10.1016/j.virusres.2022.199029. Epub 2022 Dec 22. PMID: 36565816; PMCID: PMC10194360. 2. Furutani Y, Toguchi M, Higuchi S, Yanaka K, Gailhouste L, Qin XY, Masaki T, Ochi S, Matsuura T. Establishment of a Rapid Detection System for ISG20-Dependent SARS-CoV-2 Subreplicon RNA Degradation Induced by Interferon-α. Int J Mol Sci. 2021 Oct 28;22(21):11641. doi: 10.3390/ijms222111641. PMID: 34769072; PMCID: PMC8583800. 3. Ishibashi D, Homma T, Nakagaki T, Fuse T, Sano K, Satoh K, Mori T, Atarashi R, Nishida N. Type I interferon protects neurons from prions in in vivo models. Brain. 2019 Apr 1;142(4):1035-1050. doi: 10.1093/brain/awz016. PMID: 30753318; PMCID: PMC6439327.
In vitro protocol:
1. Ruiz UEA, Santos IA, Grosche VR, Fernandes RS, de Godoy AS, Torres JDA, Freire MCLC, Mesquita NCMR, Guevara-Vega M, Nicolau-Junior N, Sabino-Silva R, Mineo TWP, Oliva G, Jardim ACG. Imidazonaphthyridine effects on Chikungunya virus replication: Antiviral activity by dependent and independent of interferon type 1 pathways. Virus Res. 2023 Jan 15;324:199029. doi: 10.1016/j.virusres.2022.199029. Epub 2022 Dec 22. PMID: 36565816; PMCID: PMC10194360. 2. Furutani Y, Toguchi M, Higuchi S, Yanaka K, Gailhouste L, Qin XY, Masaki T, Ochi S, Matsuura T. Establishment of a Rapid Detection System for ISG20-Dependent SARS-CoV-2 Subreplicon RNA Degradation Induced by Interferon-α. Int J Mol Sci. 2021 Oct 28;22(21):11641. doi: 10.3390/ijms222111641. PMID: 34769072; PMCID: PMC8583800.
In vivo protocol:
1. Ishibashi D, Homma T, Nakagaki T, Fuse T, Sano K, Satoh K, Mori T, Atarashi R, Nishida N. Type I interferon protects neurons from prions in in vivo models. Brain. 2019 Apr 1;142(4):1035-1050. doi: 10.1093/brain/awz016. PMID: 30753318; PMCID: PMC6439327.
1: Luo XL, Jiang Y, Li Q, Yu XJ, Ma T, Cao H, Ke MX, Zhang P, Tan JL, Gong YS, Wang L, Gao L, Yang HT. hESC-Derived Epicardial Cells Promote Repair of Infarcted Hearts in Mouse and Swine. Adv Sci (Weinh). 2023 Sep;10(27):e2300470. doi: 10.1002/advs.202300470. Epub 2023 Jul 28. PMID: 37505480; PMCID: PMC10520683. 2: Gao L, Liu YX, Zhou YZ, Qin XM. Baicalein Attenuates Neuroinflammation in LPS-Treated BV-2 Cells by Inhibiting Glycolysis via STAT3/c-Myc Pathway. Neurochem Res. 2023 Nov;48(11):3363-3377. doi: 10.1007/s11064-023-03961-5. Epub 2023 Jun 5. PMID: 37277556. 3: Wang X, Chen J, Shen Y, Zhang H, Xu Y, Zhang J, Cheng L. Baricitinib protects ICIs-related myocarditis by targeting JAK1/STAT3 to regulate Macrophage polarization. Cytokine. 2024 Jul;179:156620. doi: 10.1016/j.cyto.2024.156620. Epub 2024 May 2. PMID: 38701735. 4: Ruiz UEA, Santos IA, Grosche VR, Fernandes RS, de Godoy AS, Torres JDA, Freire MCLC, Mesquita NCMR, Guevara-Vega M, Nicolau-Junior N, Sabino-Silva R, Mineo TWP, Oliva G, Jardim ACG. Imidazonaphthyridine effects on Chikungunya virus replication: Antiviral activity by dependent and independent of interferon type 1 pathways. Virus Res. 2023 Jan 15;324:199029. doi: 10.1016/j.virusres.2022.199029. Epub 2022 Dec 22. PMID: 36565816; PMCID: PMC10194360. 5: Wang H, Wang S, Cheng L, Chen L, Wang Y, Qing J, Huang S, Wang Y, Lei X, Wu Y, Ma Z, Zhang L, Tang Y. Discovery of Imidazo[1,2-α][1,8]naphthyridine Derivatives as Potential HCV Entry Inhibitor. ACS Med Chem Lett. 2015 Jul 27;6(9):977-81. doi: 10.1021/acsmedchemlett.5b00159. PMID: 26396683; PMCID: PMC4569878. 6: Zhang C, Peng Q, Tang Y, Wang C, Wang S, Yu D, Hou S, Wang Y, Zhang L, Lin N. Resveratrol ameliorates glioblastoma inflammatory response by reducing NLRP3 inflammasome activation through inhibition of the JAK2/STAT3 pathway. J Cancer Res Clin Oncol. 2024 Mar 28;150(3):168. doi: 10.1007/s00432-024-05625-5. PMID: 38546908; PMCID: PMC10978631. 7: Xu Y, Tan X, Yang Q, Fang Z, Chen W. Akkermansia muciniphila outer membrane protein regulates recruitment of CD8+ T cells in lung adenocarcinoma and through JAK-STAT signalling pathway. Microb Biotechnol. 2024 Jul;17(7):e14522. doi: 10.1111/1751-7915.14522. PMID: 39016683; PMCID: PMC11253302. 8: Meng J, Zhang C, Zhu N, Zhang C, Liu M, Han Z, Li Y. EPN3 plays oncogenic role in non-small cell lung cancer by activating the JAK1/2-STAT3 pathway. Environ Toxicol. 2023 Aug;38(8):1968-1979. doi: 10.1002/tox.23822. Epub 2023 Apr 26. PMID: 37186036. 9: Wang F, Xia JJ, Shen LJ, Jiang TT, Li WL, You DL, Chang Q, Hu SY, Wang L, Wu X. Curcumin attenuates intracerebral hemorrhage-induced neuronal apoptosis and neuroinflammation by suppressing JAK1/STAT1 pathway. Biochem Cell Biol. 2022 Jun 1;100(3):236-245. doi: 10.1139/bcb-2021-0423. Epub 2022 Apr 5. PMID: 35381181. 10: Huang S, Qing J, Wang S, Wang H, Zhang L, Tang Y. Design and synthesis of imidazo[1,2-α][1,8]naphthyridine derivatives as anti-HCV agents via direct C-H arylation. Org Biomol Chem. 2014 Apr 21;12(15):2344-8. doi: 10.1039/c3ob42525h. PMID: 24595428. 11: Ishibashi D, Homma T, Nakagaki T, Fuse T, Sano K, Satoh K, Mori T, Atarashi R, Nishida N. Type I interferon protects neurons from prions in in vivo models. Brain. 2019 Apr 1;142(4):1035-1050. doi: 10.1093/brain/awz016. PMID: 30753318; PMCID: PMC6439327. 12: Jia Y, Wang W, Jiang J, Zhang X, Li H, Gong S, Li Z, Liu H, Shang C, Wang A, Jin Y, Lin P. LncRNA STAT3-AS regulates endometrial receptivity via the STAT3 signaling pathway. Theriogenology. 2024 Mar 1;216:118-126. doi: 10.1016/j.theriogenology.2023.12.032. Epub 2023 Dec 29. PMID: 38171198. 13: Xiong J, Zhou R, Deng X. PRDX6 alleviated heart failure by inhibiting doxorubicin-induced ferroptosis through the JAK2/STAT1 pathway inactivation. In Vitro Cell Dev Biol Anim. 2024 Apr;60(4):354-364. doi: 10.1007/s11626-024-00889-0. Epub 2024 Mar 26. PMID: 38530594. 14: Kitamura H, Tanigawa T, Kuzumoto T, Nadatani Y, Otani K, Fukunaga S, Hosomi S, Tanaka F, Kamata N, Nagami Y, Taira K, Uematsu S, Watanabe T, Fujiwara Y. Interferon-α exerts proinflammatory properties in experimental radiation-induced esophagitis: Possible involvement of plasmacytoid dendritic cells. Life Sci. 2022 Jan 15;289:120215. doi: 10.1016/j.lfs.2021.120215. Epub 2021 Dec 8. PMID: 34890590. 15: Lei H, Shi J, Teng Y, Song C, Zou L, Ye F, Zhang H. Baicalein modulates the radiosensitivity of cervical cancer cells in vitro via miR-183 and the JAK2/STAT3 signaling pathway. Adv Clin Exp Med. 2021 Jul;30(7):727-736. doi: 10.17219/acem/135478. PMID: 34118143. 16: Furutani Y, Toguchi M, Shiozaki-Sato Y, Qin XY, Ebisui E, Higuchi S, Sudoh M, Suzuki H, Takahashi N, Watashi K, Wakita T, Kakeya H, Kojima S. An interferon-like small chemical compound CDM-3008 suppresses hepatitis B virus through induction of interferon-stimulated genes. PLoS One. 2019 Jun 12;14(6):e0216139. doi: 10.1371/journal.pone.0216139. PMID: 31188831; PMCID: PMC6561549. 17: Ying ZH, Li HM, Yu WY, Yu CH. Iridin Prevented Against Lipopolysaccharide- Induced Inflammatory Responses of Macrophages via Inactivation of PKM2-Mediated Glycolytic Pathways. J Inflamm Res. 2021 Feb 5;14:341-354. doi: 10.2147/JIR.S292244. PMID: 33574693; PMCID: PMC7872898. 18: Furutani Y, Toguchi M, Higuchi S, Yanaka K, Gailhouste L, Qin XY, Masaki T, Ochi S, Matsuura T. Establishment of a Rapid Detection System for ISG20-Dependent SARS-CoV-2 Subreplicon RNA Degradation Induced by Interferon-α. Int J Mol Sci. 2021 Oct 28;22(21):11641. doi: 10.3390/ijms222111641. PMID: 34769072; PMCID: PMC8583800. 19: Lin L, Wang Y, Xu W, Huang C, Hu J, Chen X, Lv X, Qin Y, Zhao X, Li H. Aerobic Exercise Improves Type 2 Diabetes Mellitus-Related Cognitive Impairment by Inhibiting JAK2/STAT3 and Enhancing AMPK/SIRT1 Pathways in Mice. Dis Markers. 2022 May 5;2022:6010504. doi: 10.1155/2022/6010504. PMID: 35578689; PMCID: PMC9107038. 20: Takahashi N, Hayashi K, Nakagawa Y, Furutani Y, Toguchi M, Shiozaki-Sato Y, Sudoh M, Kojima S, Kakeya H. Development of an anti-hepatitis B virus (HBV) agent through the structure-activity relationship of the interferon-like small compound CDM-3008. Bioorg Med Chem. 2019 Feb 1;27(3):470-478. doi: 10.1016/j.bmc.2018.11.039. Epub 2018 Dec 5. PMID: 30552008.