Synonym
Mefox; (6RS)-Mefox;
IUPAC/Chemical Name
(4-(((2-amino-8-methyl-4,9-dioxo-6,7,8,9-tetrahydro-4H-pyrazino[1,2-a][1,3,5]triazin-7-yl)methyl)amino)benzoyl)-L-glutamic acid
InChi Key
AFIMZPFBSYESNQ-ABLWVSNPSA-N
InChi Code
InChI=1S/C20H23N7O7/c1-26-12(9-27-15(17(26)31)24-19(21)25-20(27)34)8-22-11-4-2-10(3-5-11)16(30)23-13(18(32)33)6-7-14(28)29/h2-5,12-13,22H,6-9H2,1H3,(H,23,30)(H,28,29)(H,32,33)(H2,21,25,34)/t12?,13-/m0/s1
SMILES Code
O=C(O)CC[C@@H](C(O)=O)NC(C1=CC=C(NCC2N(C)C(C(N3C2)=NC(N)=NC3=O)=O)C=C1)=O
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
Preparing Stock Solutions
The following data is based on the
product
molecular weight
473.44
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
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5: Fazili Z, Sternberg MR, Paladugula N, Whitehead RD Jr, Chen H, Pfeiffer CM. The loss of 5-methyltetrahydrofolate in human serum under suboptimal preanalytical conditions can only partially be recovered by an oxidation product. J Nutr. 2014 Nov;144(11):1873-9. doi: 10.3945/jn.114.198358. Epub 2014 Sep 17. PubMed PMID: 25332487; PubMed Central PMCID: PMC5332148.
6: Fazili Z, Whitehead RD Jr, Paladugula N, Pfeiffer CM. A high-throughput LC-MS/MS method suitable for population biomonitoring measures five serum folate vitamers and one oxidation product. Anal Bioanal Chem. 2013 May;405(13):4549-60. doi: 10.1007/s00216-013-6854-9. Epub 2013 Mar 6. PubMed PMID: 23462981; PubMed Central PMCID: PMC5326548.
7: Fazili Z, Pfeiffer CM. Accounting for an isobaric interference allows correct determination of folate vitamers in serum by isotope dilution-liquid chromatography-tandem MS. J Nutr. 2013 Jan;143(1):108-13. doi: 10.3945/jn.112.166769. Epub 2012 Nov 21. PubMed PMID: 23173171; PubMed Central PMCID: PMC5362735.
