MedKoo Biosciences, Inc.
Tel:   +1-919-636-5577    Fax:   +1-919-980-4831    Email:   sales@medkoo.com
Search
Login Register
Search
MedKoo Cat#: 591705 | Name: Diphenylcarbazide
Featured

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Diphenylcarbazide is used for determination of chromium.

Chemical Structure

Diphenylcarbazide
Diphenylcarbazide
CAS#140-22-7

Theoretical Analysis

MedKoo Cat#: 591705

Name: Diphenylcarbazide

CAS#: 140-22-7

Chemical Formula: C13H14N4O

Exact Mass: 242.1168

Molecular Weight: 242.28

Elemental Analysis: C, 64.45; H, 5.82; N, 23.13; O, 6.60

Price and Availability

Size Price Availability Quantity
10g USD 250.00 2 Weeks
25g USD 450.00 2 weeks
Bulk Inquiry
Buy Now
Add to Cart
Related CAS #
No Data
Synonym
Diphenylcarbazide; 1,5-Diphenylcarbazide; NSC 5058; NSC5058; NSC-5058;
IUPAC/Chemical Name
1,5-Diphenylcarbonohydrazide
InChi Key
KSPIHGBHKVISFI-UHFFFAOYSA-N
InChi Code
InChI=1S/C13H14N4O/c18-13(16-14-11-7-3-1-4-8-11)17-15-12-9-5-2-6-10-12/h1-10,14-15H,(H2,16,17,18)
SMILES Code
O=C(NNC1=CC=CC=C1)NNC2=CC=CC=C2
Appearance
Solid powder
Purity
>97% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Diphenylcarbazide is used for determination of chromium.
In vitro activity:
The developed preconcentration-speciation analysis was finished with a diphenylcarbazide (DPC) spectrophotometric procedure suitable for conventional laboratories. The resin showed excellent salt tolerance, enabling Cr analysis in seawater, and was stable over extended use. Reference: Anal Bioanal Chem. 2003 Jul;376(6):928-33. https://pubmed.ncbi.nlm.nih.gov/12819851/
In vivo activity:
TBD

Preparing Stock Solutions

The following data is based on the product molecular weight 242.28 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Maremeni LC, Modise SJ, Mtunzi FM, Klink MJ, Pakade VE. Adsorptive Removal of Hexavalent Chromium by Diphenylcarbazide-Grafted Macadamia Nutshell Powder. Bioinorg Chem Appl. 2018 Apr 19;2018:6171906. doi: 10.1155/2018/6171906. PMID: 29849541; PMCID: PMC5933043. 2. Filik H, Doğutan M, Apak R. Speciation analysis of chromium by separation on a 5-palmitoyl oxine-functionalized XAD-2 resin and spectrophotometric determination with diphenylcarbazide. Anal Bioanal Chem. 2003 Jul;376(6):928-33. doi: 10.1007/s00216-003-2006-y. Epub 2003 Jun 18. PMID: 12819851.
In vitro protocol:
1. Maremeni LC, Modise SJ, Mtunzi FM, Klink MJ, Pakade VE. Adsorptive Removal of Hexavalent Chromium by Diphenylcarbazide-Grafted Macadamia Nutshell Powder. Bioinorg Chem Appl. 2018 Apr 19;2018:6171906. doi: 10.1155/2018/6171906. PMID: 29849541; PMCID: PMC5933043. 2. Filik H, Doğutan M, Apak R. Speciation analysis of chromium by separation on a 5-palmitoyl oxine-functionalized XAD-2 resin and spectrophotometric determination with diphenylcarbazide. Anal Bioanal Chem. 2003 Jul;376(6):928-33. doi: 10.1007/s00216-003-2006-y. Epub 2003 Jun 18. PMID: 12819851.
In vivo protocol:
TBD
1: Maremeni LC, Modise SJ, Mtunzi FM, Klink MJ, Pakade VE. Adsorptive Removal of Hexavalent Chromium by Diphenylcarbazide-Grafted Macadamia Nutshell Powder. Bioinorg Chem Appl. 2018 Apr 19;2018:6171906. doi: 10.1155/2018/6171906. eCollection 2018. PubMed PMID: 29849541; PubMed Central PMCID: PMC5933043. 2: Calderilla C, Maya F, Cerdà V, Leal LO. 3D printed device for the automated preconcentration and determination of chromium (VI). Talanta. 2018 Jul 1;184:15-22. doi: 10.1016/j.talanta.2018.02.065. Epub 2018 Feb 16. PubMed PMID: 29674027. 3: Lotlikar NP, Damare SR, Meena RM, Linsy P, Mascarenhas B. Potential of Marine-Derived Fungi to Remove Hexavalent Chromium Pollutant from Culture Broth. Indian J Microbiol. 2018 Jun;58(2):182-192. doi: 10.1007/s12088-018-0719-z. Epub 2018 Mar 12. PubMed PMID: 29651177; PubMed Central PMCID: PMC5891482. 4: Faghihian H, Adibmehr Z. Comparative performance of novel magnetic ion-imprinted adsorbents employed for Cd(2+), Cu(2+) and Ni(2+) removal from aqueous solutions. Environ Sci Pollut Res Int. 2018 May;25(15):15068-15079. doi: 10.1007/s11356-018-1732-9. Epub 2018 Mar 19. PubMed PMID: 29557038. 5: Reveko V, Lampert F, Din RU, Thyssen JP, Møller P. False-positive result when a diphenylcarbazide spot test is used on trivalent chromium-passivated zinc surfaces. Contact Dermatitis. 2018 May;78(5):315-320. doi: 10.1111/cod.12955. Epub 2018 Jan 17. PubMed PMID: 29341169. 6: Hedberg YS, Erfani B, Matura M, Lidén C. Chromium(III) release from chromium-tanned leather elicits allergic contact dermatitis: a use test study. Contact Dermatitis. 2018 May;78(5):307-314. doi: 10.1111/cod.12946. Epub 2018 Jan 11. PubMed PMID: 29322530; PubMed Central PMCID: PMC5901407. 7: Asano H, Shiraishi Y. Microfluidic Paper-based Analytical Device for the Determination of Hexavalent Chromium by Photolithographic Fabrication Using a Photomask Printed with 3D Printer. Anal Sci. 2018;34(1):71-74. doi: 10.2116/analsci.34.71. PubMed PMID: 29321462. 8: Zhou Z, Kong D, Zhu H, Wang N, Wang Z, Wang Q, Liu W, Li Q, Zhang W, Ren Z. Preparation and adsorption characteristics of an ion-imprinted polymer for fast removal of Ni(II) ions from aqueous solution. J Hazard Mater. 2018 Jan 5;341:355-364. doi: 10.1016/j.jhazmat.2017.06.010. Epub 2017 Jun 8. PubMed PMID: 28802246. 9: Lu J, Xu K, Yang J, Hao Y, Cheng F. Nano iron oxide impregnated in chitosan bead as a highly efficient sorbent for Cr(VI) removal from water. Carbohydr Polym. 2017 Oct 1;173:28-36. doi: 10.1016/j.carbpol.2017.05.070. Epub 2017 May 26. PubMed PMID: 28732867. 10: Stec K. Problems of Determining the Content of Cr(VI) in Raw Materials and Materials Containing Chromite Ore. Crit Rev Anal Chem. 2017 Nov 2;47(6):469-473. doi: 10.1080/10408347.2017.1334533. Epub 2017 Jun 2. Review. PubMed PMID: 28574278. 11: Paluch J, Kozak J, Wieczorek M, Kozak M, Kochana J, Widurek K, Konieczna M, Kościelniak P. Novel approach to two-component speciation analysis. Spectrophotometric flow-based determinations of Fe(II)/Fe(III) and Cr(III)/Cr(VI). Talanta. 2017 Aug 15;171:275-282. doi: 10.1016/j.talanta.2017.05.005. Epub 2017 May 3. PubMed PMID: 28551141. 12: Miyake Y, Tokumura M, Iwazaki Y, Wang Q, Amagai T, Horii Y, Otsuka H, Tanikawa N, Kobayashi T, Oguchi M. Determination of hexavalent chromium concentration in industrial waste incinerator stack gas by using a modified ion chromatography with post-column derivatization method. J Chromatogr A. 2017 Jun 16;1502:24-29. doi: 10.1016/j.chroma.2017.04.046. Epub 2017 Apr 25. PubMed PMID: 28473202. 13: Yanykin DV, Khorobrykh AA, Terentyev VV, Klimov VV. Two pathways of photoproduction of organic hydroperoxides on the donor side of photosystem 2 in subchloroplast membrane fragments. Photosynth Res. 2017 Sep;133(1-3):129-138. doi: 10.1007/s11120-017-0373-z. Epub 2017 Mar 27. PubMed PMID: 28349346. 14: Chen L, Chen Z, Chen D, Xiong W. Removal of hexavalent chromium from contaminated waters by ultrasound-assisted aqueous solution ball milling. J Environ Sci (China). 2017 Feb;52:276-283. doi: 10.1016/j.jes.2016.04.006. Epub 2016 May 4. PubMed PMID: 28254048. 15: Ohira SI, Nakamura K, Chiba M, Dasgupta PK, Toda K. Matrix isolation with an ion transfer device for interference-free simultaneous spectrophotometric determinations of hexavalent and trivalent chromium in a flow-based system. Talanta. 2017 Mar 1;164:445-450. doi: 10.1016/j.talanta.2016.08.079. Epub 2016 Sep 6. PubMed PMID: 28107955. 16: Gor'kova T, Liu M, Proskurnin M, Franko M. Determination of Trace Cr(VI) with Diphenylcarbazide by μFIA-Thermal Lens Microscopy. Acta Chim Slov. 2016 Dec;63(4):772-780. PubMed PMID: 28004088. 17: Tiwari S, Deb MK, Sen BK. Cloud point extraction and diffuse reflectance-Fourier transform infrared spectroscopic determination of chromium(VI): A probe to adulteration in food stuffs. Food Chem. 2017 Apr 15;221:47-53. doi: 10.1016/j.foodchem.2016.10.034. Epub 2016 Oct 11. PubMed PMID: 27979229. 18: Takahashi M, Shigeto J, Sakamoto A, Morikawa H. Light-triggered selective nitration of PsbO1 in isolated Arabidopsis thylakoid membranes is inhibited by photosynthetic electron transport inhibitors. Plant Signal Behav. 2016 Dec;11(12):e1263413. doi: 10.1080/15592324.2016.1263413. PubMed PMID: 27901641; PubMed Central PMCID: PMC5225929. 19: Tahmasebi Z, Davarani SSH. Selective and sensitive speciation analysis of Cr(VI) and Cr(III), at sub-μgL(-1) levels in water samples by electrothermal atomic absorption spectrometry after electromembrane extraction. Talanta. 2016 Dec 1;161:640-646. doi: 10.1016/j.talanta.2016.09.016. Epub 2016 Sep 7. PubMed PMID: 27769459. 20: De Flora S, Camoirano A, Micale RT, La Maestra S, Savarino V, Zentilin P, Marabotto E, Suh M, Proctor DM. Reduction of hexavalent chromium by fasted and fed human gastric fluid. I. Chemical reduction and mitigation of mutagenicity. Toxicol Appl Pharmacol. 2016 Sep 1;306:113-9. doi: 10.1016/j.taap.2016.07.004. Epub 2016 Jul 9. PubMed PMID: 27404458.

View History

Monatepil free base

Monatepil free base